Abstract
Bacteriophages able to propagate on Pseudomonas strains are very common and can be easily isolated from natural environments or lysogenic strains. The development of transducing systems has allowed bacterial geneticists to perform chromosome analyses and mutation mapping. Moreover, these systems have also been proved to be a successful tool for molecular microbiologists to introduce a foreign gene or a mutation into the chromosome of a bacterial cell. This chapter provides a description of the phage methodology illustrated by Adams in 1959 and applicable to strain PAO1 derivatives.
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Acknowledgements
I wish to thank Dr Áine Fox, with whom I had the pleasure of working on several transduction experiments whilst at the University of Lausanne, for critically reviewing this book chapter and Professor Dieter Haas, my Ph.D. supervisor, for introducing me to the fascinating world of phages.
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Frangipani, E. (2014). Gene Transfer: Transduction. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_1
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DOI: https://doi.org/10.1007/978-1-4939-0473-0_1
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