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Are the duplicates of perennial kale (Brassica oleracea L. var. ramosa DC.) true duplicates as determined by RAPD analysis?

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Abstract

A collection of vegetatively propagated perennial kales (Brassica oleracea L. var. ramosa DC., 64 accessions in total), predominantly from the Netherlands, was subjected to RAPD analysis in order to confirm or reject the presence of possible duplicates as indicated by isozyme and cytological data, as well as by morphological data described here. By employing 9 decamer primers, seven RAPD patterns could be attributed to almost all of the respective accessions, with up to 22 clones exhibiting identical features for the parameters examined. By these results, a high percentage of duplication is very strongly indicated in the collection, offering possibilities for a reduction in the number of clones to be propagated. The highest level of genetic variation was found in two kales from Portugal, which differed significantly from the other accessions.

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Authors and Affiliations

  1. Department of Plant Breeding (I.v.P.), Agricultural University, P.0. Box 386 NL-6700 AJ, Wageningen, The Netherlands (e-mail: anton

    A.C. Zeven

  2. Institute of Plant Genetics and Crop Plant Research (IPK), Genebank, Corrensstrasse 3, D-06466, Gatersleben, Germany

    K.J. Dehmer, T. Gladis, K. Hammer, K. Hammer & H. Lux

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  1. A.C. Zeven
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  2. K.J. Dehmer
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  3. T. Gladis
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  4. K. Hammer
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  5. H. Lux
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Zeven, A., Dehmer, K., Gladis, T. et al. Are the duplicates of perennial kale (Brassica oleracea L. var. ramosa DC.) true duplicates as determined by RAPD analysis?. Genetic Resources and Crop Evolution 45, 105–111 (1998). https://doi.org/10.1023/A:1008670015789

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  • DOI: https://doi.org/10.1023/A:1008670015789

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