Abstract
A method is presented to determine both φ and ψ backbone angles in proteins simultaneously. This is achieved by measuring the effect on two-spin coherences of cross-correlation between 15 N-1HN and 13 \({}^{{\text{13}}}{\text{C}}^\alpha{\text{ - }}{}^{\text{1}}{\text{H}}^\alpha\) vectors. The cross-correlation rates are obtained by comparing two complementary three-dimensional experiments.
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Pelupessy, P., Chiarparin, E., Ghose, R. et al. Simultaneous determination of Ψ and Φ angles in proteins from measurements of cross-correlated relaxation effects. J Biomol NMR 14, 277–280 (1999). https://doi.org/10.1023/A:1008339928400
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DOI: https://doi.org/10.1023/A:1008339928400