Abstract
The effects of several kinds of microbial extracellular glycolipids on neurite initiation in PC12 cells were examined. Addition of mannosylerythritol lipid-A (MEL-A), MEL-B, and sophorose lipid (SL) to PC12 cells caused significant neurite outgrowth. Other glycolipids, such as polyol lipid (PL), rhamnose lipid (RL), succinoyl trehalose lipid-A (STL-A) and STL-B caused no neurite-initiation. MEL-A increased acetylcholine esterase (AChE) activity to an extent similar to nerve growth factor (NGF). However, MEL-A induced one or two long neurites from the cell body, while NGF induced many neurites. In addition, MEL-A-induced differentiation was transient, and after 48 h, percentage of cells with neurites started to decrease in contrast to neurons induced by NGF, which occurred in a time-dependent manner. MEL-A could induce neurite outgrowth after treatment of PC12 cells with an anti-NGF receptor antibody that obstructed NGF action. These results indicate that MEL-A and NGF induce differentiation of PC12 cells through different mechanisms.
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References
Chao M (1992) Growth factors signaling: where is the specificity? Cell 68: 995–997.
Cooper DG and Paddock DA (1984) Production of a biosurfactant from Torulopsis bombicola. Appl Environ Microbiol 47: 173–176.
Dicik I, Batzer AG, Blaikie P, Obermeier A, Ullrich A, Schhlessinger J and Margolis B (1995) Shc binding to nerve growth factor receptor is mediated by the phosphotyrosine interaction domain. J Biol Chem 270: 15125–15129.
Greene LA and Rukenstein A (1981) Regulation of acetylcholinesterase activity by nerve growth factor. J Biol Chem 256: 6363–6367.
Greene LA and Tischler AS (1976) Establishment of a noradrenergic clonal cell line of rat pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci US 73: 2424–2428.
Greene LA and Tischler AS (1982) PC12 pheochromocytoma cells in neurobiology research. Adv Cell Neurobiol 3: 373–414.
Hakomori S and Igarashi Y (1992) Gangliosides and glycosphingolipids as modulators of cell growth, adhesion, and transmembrane signaling. In Advances Lipid Research 25: 147–162 Academic Press, Inc.
Hempstead BL, Rabin SJ, Kaplan L, Reid S, Parada LF and Kaplan DR (1992) Overexpression of the Trk tyrosine kinase rapidly accelerates nerve growth factor-induced differentiation. Neuron 9: 883–896.
Hisatsuka K, Nakahara T, Sano N and Yamada K (1971) Formation of rhamnolipid by Pseudomonas aeruginosa and its function in hydrocarbon fermentation. Agr Biol Chem 35: 686–692.
Isoda H, Kitamoto D, Shinmoto H, Matsumura M and Nakahara T (1997) Microbial extracellular glycolipid induction of differentiation and inhibition of the protein kinase C activity of human promyelocytic leukemia cell line HL60. Biosci Biotech Biochem 71: 609–614.
Isoda H, Shinmoto H, Kitamoto D, Matsumura M and Nakahara T (1997) Differentiation of Human Promyelocytic Leukemia Cell Line HL60 by Microbial Extracellular Glycolipids. Lipids 32: 263–271.
Isoda H and Nakahara T (1997) Mannosylerythritol lipid induced granulocytic differentiation and inhibited the tyrosine phosphorylation of human myelogenous leukemia cell line K562. Cytotechnology 25: 191–195.
Kaplan DR, Martin-Zanca D and Parada LF (1991) Tyrosine phosphorylation and tyrosine kinase activity of the trk proto-oncogene product induced by NGF. Nature 350: 158–160.
Kitamoto D, Haneishi K, Nakahara T and Tabuchi T (1990) Production of mannosylerythritol lipids by Candida antarctica from vegitable oils. Agric Biol Chem 54: 37–40.
Kim UH, Fink DJ, Kim HS, Park DJ, Contreras ML, Guroff G and Rhee SG (1991) Nerve growth factor stimulates phosphorylation of phospholipase C-γ in PC12 cells. J Biol Chem 266: 1359–1362.
Klein R, Jing S, Nanduri V, O'Rourke E and Barbacid M (1991) The trk proto-oncogene encodes a receptor for nerve growth factor. Cell 65: 189–197.
Kurosawa T, Sakai K, Nakahara T, Oshima Y and Tabuchi T (1994) Extracellular accumulation of the polyol lipids, 3,5-dihydroxydecanoyl and 5-hydroxy 2-decenoyl esters of arabitol and mannitol, by Aureobasidium sp. Biosci Biotech Biochem 58: 2057–2060.
Lang S and Wagner F (1987) Structure and properties of biosurfactants. Surfactant Sci Ser 25: 21–45.
Ledden RW (1989) Biosynthesis, metabolism, and biological effects of gangliosides. Neurobiology of Glycoconjugates: 43–83.
Lowry OH, Rosebrough NJ, Farr HG and Randall RJ (1951) Protein measurement with the Folinphenol reagent. J Biol Chem 193: 265–275.
Maher P (1988) Nerve growth factor induces protein-tyrosine phosphorylation. Proc Natl Acad Sci USA 85: 6788–6791.
Saito Y and Kawashima S (1989) The neurite-initiating effect of a tripeptide aldehyde protease inhibitor on PC12h cells. J Biochem 106: 1035–1040.
Soltoff SP, Rabin SL, Cantley LC and Kaplan DR (1992) Nerve growth factor promotes the activation of phosphatidylinositol 3-kinase and its association with the trk tyrosine kinase. J Biol Chem 267: 17472–17477.
Thomas SM, DeMarco M, D'Arcangelo G, Armstrong RC, Meyer BE, Zilberstein A, Brugge AS and Halegoua S (1992) Induction of neurite outgrowth by v-src mimics critical aspects of nerve growth factor-induced differentiation. Mol Cell Biol 11: 4739–4750.
Tsuji S, Yamashita T and Nagai Y (1988) A novel, carbohydrate signal-mediated cell surface protein phosphorylation: ganglioside GQ1b stimulates ecto-protein kinase activity on the cell surface of a human neuroblastoma cell line, GOTO. J Biochem 104: 498–503.
Uchida Y, Misawa S, Nakahara T and Tabuchi T (1989) Factors affecting the production of succinoyl trehalose lipids by Rhodococcus erythropolis grown on n-alkanes. Agric Biol Chem 53: 765–769.
Uchida Y, Tsuchiya R, Chino M, Hirano J and Tabuchi T (1989) Extracellular Accumulation of mono-and di-Succinoyl Trehalose Lipids by a Strain of Rhodococcus erythropolis SD-74 Grown on n-Alkanes. Agric Biol Chem 53: 757–763.
Vetter ML, Martin-Zanca D, Parada LF, Bishop JM and Kaplan DR (1991) Nerve growth factor rapidly stimulates tyrosine phosphorylation of phospholipase C-γ 1 by a kinase activity associated with the produt of the trk protooncogene. Proc Natl Acad Sci USA 88: 5650–5654.
Wood KW, Sarnecki C, Roberts TM and Blenis J (1992) Ras mediates nerve growth factor receptor modulation of three signal transduction protein kinases: MAP kinase, Raf-1 and RSK. Cell 68: 1041–1050.
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Isoda, H., Shinmoto, H., Matsumura, M. et al. The neurite-initiating effect of microbial extracellular glycolipids in PC12 cells. Cytotechnology 31, 165–172 (1999). https://doi.org/10.1023/A:1008020121693
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DOI: https://doi.org/10.1023/A:1008020121693