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Expansion of human autologous cytotoxic T lymphocytes on fixed target tumor cells

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Abstract

Human tumor specific cytotoxic T lymphocytes (CTL) were expanded on formalin-fixed autologous target tumor cells derived from glioblastoma multiforme. Growth response of the CTL restimulated with the fixed target cells was larger than those with live target cells. The results suggest that formalin-fixed tumor cells will be stable sources of tumor antigen for efficient autologous CTL expansion and be useful for adoptive immunotherapy of tumors.

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Authors and Affiliations

  1. RIKEN Cell Bank, The Institute of Physical and Chemical Research (RIKEN) Institute of Applied Biochemistry, Japan

    Ken-ichi Horiuchi, Hideo Tsurushima, Berm Soo Kim, Shu Qin Liu, Kaoru Saijo, Nakao Nomura & Tadao Ohno

  2. Department of Neurosurgery, Institute of Clinical Medicine, University of Tsukuba, Tsukuba Science City, Japan

    Hideo Tsurushima

  3. Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan

    Yasuo Saijo & Toshihiro Nukiwa

  4. Institute of Applied Biochemistry, Japan

    Nakao Nomura & Masatoshi Matsumura

Authors
  1. Ken-ichi Horiuchi
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  2. Hideo Tsurushima
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  3. Berm Soo Kim
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  4. Shu Qin Liu
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  5. Kaoru Saijo
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  6. Yasuo Saijo
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  7. Toshihiro Nukiwa
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  8. Nakao Nomura
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  9. Masatoshi Matsumura
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  10. Tadao Ohno
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Horiuchi, Ki., Tsurushima, H., Soo Kim, B. et al. Expansion of human autologous cytotoxic T lymphocytes on fixed target tumor cells. Cytotechnology 26, 119–124 (1998). https://doi.org/10.1023/A:1007903614475

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  • DOI: https://doi.org/10.1023/A:1007903614475

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