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Effect of dietary selenium, zinc and allopurinol supplements on plasma and tissue manganese levels in rats with thiocetamide-induced liver cirrhosis

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Abstract

The effect of thiocetamide-induced liver cirrhosis on plasma and tissuemanganese levels and the protective role of selenium, zinc and allopurinolsupplements was investigated in rats. Control plasma and liver manganese(Mn) levels were found to be (mean ± SD): 8.4 ± 2.4 mg/L and5.7 ± 1.5 mg/g wet weight respectively. Plasma manganese levels weresignificantly increased (p < 0.001) whereas liver manganese levels weresignificantly reduced (p < 0.05) in the cirrhotic rats. Treatment withselenium, zinc and allopurinol reversed this trend and restored themanganese levels close to the normal values. Lung, spleen, and kidneymanganese levels under control conditions were considerably lower than thatof the liver tissue. However, these levels registered a significant increase(p < 0.05) in cirrhotic rats and this change was normalized after selenium,zinc and allopurinol treatment. There were no significant differences in thecomparative efficacy of each of these protective agents. Zinc supplementconsiderably increased the plasma zinc levels and plasma Zn/Mn ratio had agood correlation with plasma zinc concentration. This ratio wassignificantly reduced in cirrhotic rats, but returned to the control levelafter zinc, selenium and allopurinol treatment. The results of this studyindicate that the trace element, manganese, plays an important role instabilizing cell structure and that this effect is mediated possibly bypreserving the antioxidant activity of the tissues.

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Authors and Affiliations

  1. Department of Pathology, Faculty of Medicine, Kuwait University Health Sciences Center, Kuwait

    A.A. Al-Bader

  2. Shamiya Clinic, Ministry of Public Health, Kuwait

    T.A. Hussain

  3. Department of Surgery, Faculty of Medicine, Kuwait University Health Sciences Center, Kuwait

    H.M. Dashti

  4. Qadessia Clinic, Ministry of Public Health, Kuwait

    M.H. Mosawi

Authors
  1. A.A. Al-Bader
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  2. H.M. Dashti
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  3. M.H. Mosawi
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  4. T.A. Hussain
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Al-Bader, A., Dashti, H., Mosawi, M. et al. Effect of dietary selenium, zinc and allopurinol supplements on plasma and tissue manganese levels in rats with thiocetamide-induced liver cirrhosis. Mol Cell Biochem 173, 121–125 (1997). https://doi.org/10.1023/A:1006835912530

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