Abstract
We show that tetraphenylphosphonium inhibits oxidation of palmitoylcarnitine, pyruvate, malate, 2-oxoglutarate and glutamate in heart mitochondria in the range of concentration (1–5 µM) commonly used for the determination of mitochondrial membrane potential. The inhibition of 2-oxoglutarate (but not other substrate) oxidation by tetraphenylphosphonium is dependent on the concentration of 2-oxoglutarate and on extramitochondrial free calcium, and the kinetic plots are consistent with a mixed type of inhibition. Our results indicate that tetraphenylphosphonium interacts with enzymes, specifically involved in the oxidation of 2-oxoglutarate, most possibly, 2-oxoglutarate dehydrogenase.
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Mildaziene, V., Baniene, R., Marcinkeviciute, A. et al. Tetraphenylphosphonium inhibits oxidation of physiological substrates in heart mitochondria. Mol Cell Biochem 174, 67–70 (1997). https://doi.org/10.1023/A:1006812128286
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DOI: https://doi.org/10.1023/A:1006812128286