Abstract
We have previously reported that transfection of mouse interferon γ (IFN-γ) in H-2K k -positive BW vari-ants (BW-Sp3) abolishes tumorigenicity and reduces metastatic capacity. To further increase the immuno-genicity of BW-Sp3 cells, the gene for human interleukin 2 (huIL-2) was transfected in these cancer cells. Single BW-Sp3(huIL-2) and double BW-Sp3(huIL-2+IFN-γ) transfectants were generated and their behavior was investigated as compared to parental and IFN-γ-transfected BW-Sp3. Although expression of huIL-2 was equally effective as IFN-γ in preventing tumor formation and reducing experimental metastasis, it did not confer protection to spontaneous metastases and even reversed the anti-metastatic activity of IFN-γ. Inoculation of the BW variants in immunocompromised mice revealed that expression of IL-2 activates both T cells and aspecific immune effectors. However, in immunocompromised mice a clear pro-metastatic effect of IL-2 was recorded. Analysis of membrane antigens on the different variants showed a selective effect of huIL-2 on the expression of two surface antigens, i.e. L-selectin and metastatic T cell hybridoma antigen (MTH), which may contribute to metastasis. Hence upon expression of huIL-2 in T lymphoma variants, tumor outcome will depend on the balanced effects of the transfected cytokines on the immune response and the redirected effect on tumor progression. © Rapid Science Ltd.
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Geldhof, A.B., Van Ginderachter, J., Vandersteen, P. et al. Multiple effects of transfection with interleukin 2 and/or interferon γ on the behavior of mouse T lymphoma cells. Clin Exp Metastasis 16, 447–459 (1998). https://doi.org/10.1023/A:1006585525399
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DOI: https://doi.org/10.1023/A:1006585525399