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Thermoluminescence and fluorescence study of changes in Photosystem II photochemistry in desiccating barley leaves

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Abstract

An effect of desiccation (a decrease of relative water content from 97% to 10% within 35 h) on Photosystem II was studied in barley leaf segments (Hordeum vulgare L. cv. Akcent) using chlorophyll a fluorescence and thermoluminescence (TL). The O-J-I-P fluorescence induction curve revealed a decrease of FP and a slight shift of the J step to a shorter time with no change in its height. The analysis of the fluorescence decline after a saturating light flash revealed an increased portion of slow exponential components with increasing desiccation. The TL bands obtained after excitation by continuous light were situated at about –27°C (Zv band – recombination of P680+QA −), –14 °C (A band – S3QA −), +12 °C (B band – S2/3QB −) and +45 °C (C band – TyrD+QA −). The bands related to the S-states of oxygen evolving complex (A and B) were reduced by desiccation and shifted to higher and lower temperatures, respectively. In accordance with this, the band observed at about +27 °C (S2QB −) after excitation by 1 flash fired at –10 °C and band at about +20 °C (S2/3QB −) after 2 flashes decreased with increasing water deficit and shifted to lower temperatures. A new band around 5 °C appeared in both regimes of TL excitation for a relative water content of under 42% and was attributed to the Q band (S2QA −). It is suggested that under desiccation, an inhibition of the formation of S2- and S3-states in OEC occurred simultaneously with a lowering of electron transport on the acceptor side of PS II. The temperature down-shift of the TL bands obtained after the flash excitation was induced at the initial phases of water stress, indicating a decrease of the activation energy for the S2/3QB −recombination.

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Authors and Affiliations

  1. Department of Experimental Physics, Faculty of Sciences, Palacky University, tř. Svobody 26, 771 46, Olomouc, Czech Republic

    Jiří Skotnica

  2. Department of Experimental Physics, Faculty of Sciences, Palacky University, tř. Svobody 26, 771 46, Olomouc, Czech Republic

    Martina Matoušková, Jan Nauš & Lubomír Dvořák

  3. Photon Systems Instruments, Koláčkova 39, 621 00, Brno, Czech Republic

    Dušan Lazár

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  1. Jiří Skotnica
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  2. Martina Matoušková
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  5. Lubomír Dvořák
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Correspondence to Jiří Skotnica.

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Skotnica, J., Matoušková, M., Nauš, J. et al. Thermoluminescence and fluorescence study of changes in Photosystem II photochemistry in desiccating barley leaves. Photosynthesis Research 65, 29–40 (2000). https://doi.org/10.1023/A:1006472129684

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