Abstract
We have measured thermoluminescence (TL) and chlorophyll fluorescence from leaves of peas grown under an intermittent light regime (IML) and followed changes in those leaves during greening. IML peas show low variable fluorescence and a certain capacity for reversible non-photochemical quenching. It has been suggested that reversible quenching may be caused by pH-dependent release of Ca2+ from Photosystem II (PS II) (Krieger and Weis (1992) Photosynthetica 27: 89–98). Under conditions in which reversible non-photochemical quenching occurs, a TL band at around 50 °C is observed, in the presence of DCMU, in IML leaves. A band in this temperature range has previously been observed in PS II depleted of Ca2+ (Ono and Inoue (1989) Biochimica et Biophysica Acta 973: 443–449). The 50 °C band disappears upon dark adaptation. In mature leaves, no significant band is seen at 50 °C. It is concluded that, in IML leaves, reversible quenching may be related to the release of Ca2+ from Photosystem II. However, it seems that in the mature system, under most conditions, such release does not contribute significantly to quenching
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Abbreviations
- Fo:
-
dark level of fluorescence
- Fm:
-
maximal fluorescence
- Fv:
-
variable fluorescence
- IML:
-
intermittent light
- TL:
-
thermoluminescence
- Tyr:
-
tyrosine
References
Argyroudi-Akoyunoglou JH and Akoyunoglou G (1970) Photoinduced changes in the chlorophyll a to chlorophyll b ratio in young bean plants. Plant Physiol 46: 247–249
Akoyunoglou G (1981) Assembly of functional components in chloroplast photosynthetic membranes. In: Akoyunoglou G (ed) Proceedings of the Fifth International Congress on Photosynthesis, Vol V, pp 353–366. Balaban Int Science Service, Philadelphia
Armond PA, Arntzen CJ, Briantais J-M and Vernotte C (1976) Differentiation of chloroplast lamellae. Arch Biochem Biophys 175: 54–63
Bilger W and Björkman O (1991) Temperature depenence of violaxanthin deepoxidation and non-photochemical fluorescence quenching in intact leaves ofGossypium hirsutum L. andMalva pariflora L. Planta 91: 542–551
Boussac A, Sétif P and Rutherford AW (1992) Inhibition of tyrosine Z photooxidation after formation of the S3-state in Ca2+-depleted and Cl−-depleted Photosystem II. Biochemistry 31: 1224–1233
Briantais JM (1994) Light harvesting chlorophyll a-b complex requirement for regulation of Photosystem II photochemistry by non-photochemical quenching. Photosynth Res 40 (3): 287–294
Briantais JM, Venotte C, Picaud M and Krause GH (1979) A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts. Biochim Biophys Acta 548: 128–138
Day DA, Ryrie IJ and Fuad N (1984) Investigations of the role of the main light-harvesting chlorophyll-protein complex in thylakoid membranes. Reconstitution of depleted membranes from intermittent-light-grown plants with the isolated complex. J Cell Biol 97: 163–172
Demmig-Adams B (1990) Carotinoids and photoprotection in plants: A role of the xanthophyll zeaxanthin. Biochim Biophys Acta 1020: 1–24
Dubertet G and Lefort-Tran M (1981) Functional and structural organization of chlorophyll in the developing photosynthetic membranes ofEuglena gracilis Z. Biochim Biophys Acta 634: 52–69
Ducruet J-M and Miranda T (1992) Graphical and numerical analysis of thermoluminescence and fluorescence Fo emission in photosynthetic material. Photosynth Res 33: 15–28
Foyer C, Furbank R, Harbinson J and Horton P (1990) The mechanisms contributing to photosynthetic control of electron transport by carbon assimilation in leaves. Photosynth Res 25: 83–100
Genty B, Briantais J-M and Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990: 87–92
Homann PH and Madabusi LV (1993) Modification of the thermoluminescence properties of Ca2+ depleted Photosystem II membranes by the 23 kDa extrinsic polypeptide and oligocarboxylic acids. Photosynth Res 35: 29–40
Horton P and Ruban AV (1992) Regulation of Photosystem II. Photosynth Res 34: 375–386
Horton P, Ruban AV, Rees D, Pascal AA, Noctor G and Young AJ (1991) Control of the light-harvesting function of chloroplast membranes by aggregation of the LHC II chlorophyll-protein complex. FEBS Lett 292: 1–4
Ichikawa T, Inoue Y and Shibata K (1975) Characteristics of thermoluminescence bands of intact leaves and isolated chloroplasts in relation to the water-splitting activity of photosynthesis. Biochim Biophys Acta 408: 228–239
Inoue Y, Ichikwa T and Shibata K (1976) Development of thermoluminescence bands during greening of wheat leaves under continuous and intermittent illumination. Photochem Photobiol 23: 125–130
Jahns P and Junge W (1992a) Thylakoids from pea seedlings grown under intermittent light: Biochemical and flashspectrophotometric properties. Biochemistry 31: 7390–7397
Jahns P and Junge W (1992b) Proton release during the four steps of photosynthetic water oxidation: Induction of 1:1:1:1 pattern due to lack of chlorophyll a/b binding proteins. Biochemistry 31: 7398–7403
Jahns P and Krause GH (1993) Xanthophyll cycle and energy dependent fluorescence quenching in leaves from pea plants grown under intermittent light. Planta 192: 176–182
Johnson GN, Boussac A and Rutherford AW (1993) The origin of 40–50 °C thermoluminescence bands in Photosystem II. Biochim Biophys Acta 1184: 85–92
Krause GH and Behrend (1986) W pH dependent chlorophyll chlorophyll fluorescence quenching indicating a mechanism of protection against photoinhibition of chloroplasts. FEBS Lett 200: 298–302
Krause GH and Weis E (1991) Chlorophyll fluorescence and photosynthesis: The basics. Ann Rev Plant Physiol Plant Mol Biol 42: 313–349
Krieger A and Weis E (1992) Energy-dependent quenching of chlorophyll-a-fluorescence: The involvement of proton-calcium exchange at Photosystem 2. Photosynthetica 27: 89–98
Krieger A and Weis E (1993) The role of calcium in the pH-dependent control of Photosystem II. Photosynth Res 37: 117–130
Krieger A, Weis E and Demeter S (1993) Low-pH-induced Ca2+ ion release in the water-splitting system is accompanied by a shift in the midpoint redox potential of the primary quinone acceptor QA. Biochim BiophysActa 1144: 411–418
Marquardt J and Bassi R (1993) Chorophyll-proteins from maize seedlings gron under intermittent light conditions. Planta 191: 265–273
Ono T and Inoue Y (1989) Removal of Ca by pH 3.0 treatment inhibits S2 to S3 transition in photosynthetic oxygen evolution system. Biochim Biophys Acta 973: 443–449
Polle A and Junge W (1986) The slow rise of the flash-induced alkalization by Photosystem II of the suspension medium of thylakoids is reversible related to thylakoid stacking. Biochim Biophys Acta 848: 257–264
Rees D, Noctor G and Horton P (1990) The effect of high-energystate excitation quenching on maximum and dark level chlorophyll fluorescence yield. Photosynth Res 25: 199–211
Rees D, Noctor G, Ruban AV, Crofts J, Young A and Horton P (1992) pH-dependent chlorophyll quenching in spinach thylakoids from light treated or dark adapted leaves. Photosynth Res 31: 11–19
Rutherford AW, Crofts AR and Inoue Y (1982) Thermoluminescence as a probe of Photosystem II photochemistry. The origin of the flash-induced glow peaks. Biochim. Biophys. Acta 682: 457–465
Sane PV and Rutherford AW (1986) Thermoluminescence from photosynthetic membranes. In: Govindjee J and Amesz DC (eds) Fork Light Emission by Plants and Bacteria, pp 329–362. Academic Press, London
Schreiber U and Neubauer C (1987) The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination: II. Partial control by the Photosystem II donor side and possible ways of interpretation. Z Naturforsch Teil C 42: 1255–1264
Van Kooten O and Snel JFH (1990) The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosynth Res 25: 147–150
Vass I and Inoue Y (1992) Thermoluminescence in the study of Photosystem II. In: Barber J (ed) Topics in Photosynthesis, Vol 11, pp 259–294. Elsevier, Amsterdam
Walters RG and Horton P (1991) Resolution of components of non-photochemical chlorophyll fluorescence quenching in barley leaves. Photosynth Res 27: 121–133
Weis E and Berry JA (1987) Quantum efficiency of Photosystem II in relation to energy-dependent quenching of chlorophyll fluorescence. Biochim Biophys Acta 894: 198
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Johnson, G., Krieger, A. Thermoluminescence as a probe of Photosystem II in intact leaves: Non-photochemical fluorescence quenching in peas grown in an intermittent light regime. Photosynth Res 41, 371–379 (1994). https://doi.org/10.1007/BF02183039
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DOI: https://doi.org/10.1007/BF02183039