Abstract
Uptake and metabolism of 6-benzyladenine (BA) werestudied in Actinidia deliciosa explants grown onventilated liquid Murashige and Skoog medium, usingcellulose plugs as explant support, after 0.5, 1, 2,8, 16, and 24h, 15 and 35 days of exposure to 14.8 kBqof 8-[14C] BA. Absorption of the BA is a rapidprocess and in the first half hour of culture 65% ofthe initial amount had disappeared from the medium.Exogenous BA was transformed into 7-β-D-glucopyranosyl-BA, 9-β-D-glucopyranosyl-BA, [9G]BA, 3-β-D-glucopyranosyl-BA, 9-β-D-ribofuranosyl-BA, [9R]BA, adenine, adenosine,and 5′-monophosphate of [9R]BA, [9R-5′P]BA. During thefirst 8h BA levels in the culture medium decreased,being converted into the active forms [9R]BA and[9R-5′P]BA. The excess of BA absorbed was inactivatedby glucosidation yielding [9G]BA. Ventilated culturesfavoured metabolization of active forms into inactivecompounds and this could be related to the lack ofhyperhydric shoots.
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Cañal, M., Fernández, H., Fernández, P. et al. Uptake and metabolism of 6-benzyladenine in ventilated cultures of kiwifruit plants. Plant Growth Regulation 30, 209–214 (2000). https://doi.org/10.1023/A:1006328819324
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DOI: https://doi.org/10.1023/A:1006328819324