Abstract
Regeneration and development of shoots from sunflower cotyledons were improved by optimizing explant age, plant growth regulator concentrations, and duration of exposure to plant growth regulators during shoot initiation, development and rooting. Shoot initiation required only a brief exposure to auxin and cytokinin, and minimizing the duration of exposure to high levels of plant growth regulators improved shoot development. Rooting was improved in terms of both the number of shoots that rooted and the time required for rooting by incorporating activated charcoal in the lower layer of a 2-layer rooting medium.
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Alibert G, Aslane-Chanab JC & Burns M (1994) Sunflower tissue and cell cultures and their use in biotechnology. Plant Physiol. Biochem. 32: 31–44
Badea E, Prisecaru M & Angheluta H (1989) Studies on gynogenesis in intraespecific and interspecific hybrids in the genus Helianthus. Cercetari de Genetica Vegetala si Animala 1: l77-l83
Bidney D, Scelonge C, Martich J, Burrus M, Sims L & Huffman G (1992) Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens. Plant Mol. Biol. 18: 301–313
Bohorova N, Atanassov A & Georgieva-Todorova J (1985) In vitro organogenesis, androgenesis and embryo culture in the genus Helianthus L. Z. Pflanzenzuchtg. 95: 35–44
Burrus M, Chanabe C, Alibert G & Bidney D (1991) Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.). Plant Cell Rep. 10: 161–166
Ceriani MF, Hopp HE, Hahne G & Escandon AS (1992) Cotyledons: an explant for routine regeneration of sunflower plants. Plant Cell Physiol. 33: 157–164
Chraibi KMB, Castelle J-C, Latche A, Roustan J-P & Fallot J (1992a) A genotype-independent system of regeneration from cotyledons of sunflower (Helianthus annuus L.). The role of ethylene. Plant Sci. 86: 215–221
Chraibi KMB, Castelle J-C, Latche A, Roustan J-P & Fallot J (1992b) Enhancement of shoot regeneration potential by liquid medium culture from mature cotyledons of sunflower (Helianthus annuus L.). Plant Cell Rep. 10: 617–620
Chraibi KMB, Latche A, Roustan J-P & Fallot J (1991) Stimulation of shoot regeneration from cotyledons of Helianthus annuus by the ethylene inhibitors, silver and cobalt. Plant Cell Rep. 10: 204–207
Cooley GL & Wilcox A (1987) Sunflower regeneration through embryogenesis. United States Patent 4: 670, 392
Dong JZ & Jia SR (1991) High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris Scharad.). Plant Cell Rep. 9: 559–562
Espinasse A & Lay C (1989) Shoot regeneration of callus derived from globular to torpedo embryos from 59 sunflower genotypes. Crop Sci. 29: 201–205
Espinasse A, Lay C & Volin J (1989) Effects of hormone concentration and explant size on shoot organogenesis from callus derived from zygotic embryos of sunflower (Helianthus annuus L.). Plant Cell Tiss. Org. Cult. 17: l7l-l8l
Finer JJ (1987) Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.) on a high sucrose-containing medium. Plant Cell Rep. 6: 372–374
Fischer C, Klethi P & Hahne G (1992) Protoplasts from cotyledon and hypocotyl of sunflower (Helianthus annuus L.): shoot regeneration and seed production. Plant Cell Rep. 11: 632–636
Freyssinet M & Freyssinet G (1988) Plant regeneration from sun-flower (Helianthus annuus L.) immature embryos. Plant Sci. 56: 177–181
Gélébart P & San L (1987) Obtention de plantes haploïdes par culture in vitro d'ovaires et d'ovules non fécodés de tournesol (Helianthus annuus L.). Agronomie 7: 81–86
Greco B, Tanzarella OA, Carozzo G & Blanco A (1984) Callus induction and shoot regeneration in sunflower (Helianthus annuus L.). Plant Sci. Lett. 36: 73–77
Henrickson CE (1954) The flowering of sunflower explants in aseptic culture. Plant Physiol 29: 536–538
Hunold R, Burrus M, Bronner R, Duret J-P & Hahne G (1995) Transient gene expression in sunflower (Helianthus annuus L.) following microprojectile bombardment. Plant Sci. 105: 95–109
Jeannin G, Bronner R & Hahne G (1995) Somatic embryogenesis and organogenesis induced on the immature zygotic embryo of sunflower (Helianthus annuus L) cultivated in vitro: role of the sugar. Plant Cell Rep. 15: 200–204
Knittel N, Gruber V, Hahne G & Lenee P (1994) Transformation of sunflower (Helianthus annuus L.): a reliable protocol. Plant Cell Rep. 14: 81–86
Knittel N, Escandon AS & Hahne G (1991) Plant regeneration at high frequency from mature sunflower cotyledons. Plant Sci. 73: 219–226
Krasnyanski S & Menczel L (1993) Somatic embryogenesis and plant regeneration from hypocotyl protoplasts of sunflower (Helianthus annuus L.) Plant Cell Rep. 12: 260–263
Lupi MC, Bennici A, Locci F & Gennai D (1987) Plantlet formation from callus and shoot-tip culture of Helianthus annuus (L.). Plant Cell Tiss. Org. Cult. 11: 47–55
Malone-Schoneberg J, Scelonge CJ, Burrus M & Bidney DL (1994) Stable transformation of sunflower using Agrobacterium and split embryogenic axis explants. Plant Sci. 103: 199–207
McCann AW, Cooley G & van Dresser J (1988) A system for routine plantlet regeneration of sunflower (Helianthus annuus L.) from immature embryo-derived callus. Plant Cell Tiss. Org. Cult. 14: 103–110
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nestares G, Zorzoli R, Mroginski L & Picardi L (1996) Plant regeneration from cotyledons derived from mature sunflower seeds. Helia 19: 107–112
Palmer CE (1992) Enhanced shoot regeneration from Brassica campestris by silver nitrate. Plant Cell Rep. 11: 541–545
Paterson KE (1984) Shoot tip culture of Helianthus annuus-flowering and development of adventitious and multiple shoots. Amer. J. Bot. 71: 925–931
Paterson Robinson KE & Adams DO (1987) The role of ethylene in the regeneration of Helianthus annuus (sunflower) plants from callus. Physiol. Plant. 71: 151–156
Paterson KE & Everett NP (1985) Regeneration of Helianthus annuus inbred plants from callus. Plant Sci. 42: 126–132
Pelissier B, Bouchfra O, Pepin R & Freyssinet G (1990) Production of isolated somatic embryos from sunflower thin layers. Plant Cell Rep. 9: 47–50
Power CJ (1987) Organogenesis from Helianthus annuus inbreds and hybrids from the cotyledons of zygotic embryos. Amer. J. Bot. 74: 497–503
Prado E & Berville A (1990) Induction of somatic embryo development by liquid culture in sunflower (Helianthus annuus L.). Plant Sci. 67: 73–82
Pugliesi C, Cecconi F, Mandolfo A & Baroncelli S (1990) Plant regeneration and genetic variability from tissue cultures of sun-flower (Helianthus annuus L.). Plant Breeding 106: 114–12l
Robinson KEP & Everett NP (1990) Sunflower (Helianthus annuus L.): Establishment of cultures, transformation, and the regeneration of plants. In: Bajaj YPS (ed), Biotechnology in Agriculture and Forestry, Vol 10, Legumes and Oilseed Crops (pp. 434–452)
Santos KGB, Mundstock E & Bodanese-Zanettini MH (1997) Genotype-specific normalization of soybean embryogenesis through the use of an ethylene inhibitor. Plant Cell Rep. 16: 859–864
Sarrafi A, Bolandi AR, Berville A & Alibert G (1996) Analysis of cotyledon culture to measure genetic variability for organogenesis parameters in sunflower (Helianthus annuus L.). Plant Sci. 121: 213–219
Schrammeijer B, Sijmons PC, van den Elzen PJM & Hoekema A (1990) Meristem transformation of sunflower via Agrobacterium. Plant Cell Rep. 9: 55–60
Sutter EG (1996) General lab requirements, media and sterilization methods. In: Trigiano RN & Gray DJ (eds) Plant Tissue Culture Concepts and Laboratory Exercises (pp.11–25), CRC Press, New York
Thengane SR, Joshi MS, Khuspe SS & Mascarenhas AF (1994) Anther culture in Helianthus annuus L., influence of genotype and culture conditions on embryo induction and plant regeneration. Plant Cell Rep. 13: 222–226
Wingender R, Henn H-J, Barth S, Voeste D, Machlab H & Schnabl H (1996) A regeneration protocol for sunflower (Helianthus annuus L.) protoplasts. Plant Cell Rep. 15: 742–745
Witrzens B, Scowcroft WR, Downes RW & Larkin PJ (1988) Tissue culture and plant regeneration from sunflower (Helianthus annuus) and interspecific hybrids (H tuberosus x H.annuus). Plant Cell Tiss. Org. Cult. 13: 61–76
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Baker, C., Muñoz-Fernandez, N. & Carter, C. Improved shoot development and rooting from mature cotyledons of sunflower. Plant Cell, Tissue and Organ Culture 58, 39–49 (1999). https://doi.org/10.1023/A:1006306111905
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DOI: https://doi.org/10.1023/A:1006306111905