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Meristem transformation of sunflower via Agrobacterium

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Summary

For transformation of sunflower (Helianthus annuus L. cv. Zebulon), shoot apical meristems were dissected from seeds and cocultivated with a disarmed Agrobacterium tumefaciens strain harboring a binary vector carrying genes encoding GUS- and NPTII-activity. The influence of the media conditions, the time of cocultivation and the stage of the developing seed on shoot development and meristem transformation was analysed. Transformants were selected by their ability to grow on kanamycin. Transformation was confirmed by assays for GUS and NPTII. GUS-positive shoots were rooted on rockwool and transferred to soil. Transformation of shoot meristem cells occurred at low frequencies. Chimaeric expression of the two genes was observed in transformed plants. Integration of the foreign DNA in the sunflower genome was confirmed with the polymerase chain reaction.

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Abbreviations

GUS:

ß-Glucuronidase

NPTII:

Neomycin phosphotransferase II

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Authors and Affiliations

  1. MOGEN International NV, Einsteinweg 97, 2333 CB, Leiden, The Netherlands

    Barbara Schrammeijer, Peter C. Sijmons, Peter J. M. van den Elzen & André Hoekema

Authors
  1. Barbara Schrammeijer
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  2. Peter C. Sijmons
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  3. Peter J. M. van den Elzen
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  4. André Hoekema
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Communicated by H. Lörz

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Schrammeijer, B., Sijmons, P.C., van den Elzen, P.J.M. et al. Meristem transformation of sunflower via Agrobacterium . Plant Cell Reports 9, 55–60 (1990). https://doi.org/10.1007/BF00231548

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  • DOI: https://doi.org/10.1007/BF00231548

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