Abstract
The origin of somatic embryos derived from rhizome explants of triploid Musa cv. Grand Nain was the subject of histological studies during different phases of ontogenetic development. The investigation revealed that the majority of somatic embryos showed normal root formation and consisted of highly vacuolated cells in the poorly structured shoot apex. The embryogenic mass and somatic embryos were mostly derived from several morphologically competent cells. Single cell origin depended on the presence of organogenetically functional vascular cells of rhizome explants and occurred infrequently. The implications of these findings for genetic improvement of banana and plantain by in vitro mutation breeding and gene technology are discussed.
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References
Button J, Kochba J & Bornman CH (1974) Fine structure of and embryoid development from embryogenic ovular callus of 'shamouti' orange (Citrus sinensis Osb. ). J. Exp. Bot. 25: 446–457
Banerjee N, Schoofs J, Hollevoet S, Dumortier F & De Langhe E (1987) Aspects of prospects of somatic embryogenesis in Musa, ABB, cv. Bluggoe. Acta Hort. 212: 727–730
Cronauer-Mitra SS & Krikolian AP (1983) Somatic embryos from cultured tissues of triploid plantains ('ABB'). Plant Cell Rep. 2: 289–291
Cronauer-Mitra SS & Krikolian AD (1984) Multiplication of Musa from excised stem tips. Ann. Bot. 53: 321–328
Cronauer-Mitra SS & Krikolian AD (1987) Adventitious shoot production from calloid structures of banana. Plant Cell Rep. 6: 443–445
Cronauer-Mitra SS & Krikolian AD (1988) Plant regeneration via somatic embryogenesis in the seed diploid Musa ornata Roxb. Plant Cell Rep. 7: 23–25
Dhed'a D, Dumortier Francoise Panis B, Vuylsteke D & De Langhe E (1991) Plant regeneration in cell suspension cultures of the banana cv. 'Bluggoe' (Musa spp. ABB group). Fruits 46: 125–135
Escalant JV & Teisson C (1988) Embryogèn´ese somatique chez Musa sp. Comptes Rendus de l'Academie des Sciences. Paris Serie. III, 306: 277–281
Escalant JV & Teisson C (1989) Somatic embryogenesis and plants from immature zygotic embryos of the species Musa acuminata and Musa balbisiana. Plant Cell Rep. 7: 665–668
Escalant JV, Teisson C & Cote F (1994) Amplified somatic embryogenesis from male flowers of triploid banana and plantain cultivars (Musa spp.). In Vitro Cell Dev. Biol. 30: 181–186
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean roots cells. Exp. Cell Res. 50: 151–158
Goebel-Tourand I, Mauro MC, Sossountzov L, Miginiac L & Deloire A (1993) Arrest of somatic embryo development in grapevine: histological charcterization and the effects of ABA, BAP and zeatin in stimulating plantlet development. Plant Cell Tiss. Org. Cult. 33: 91–103
Jarret RL, Fisher JB & Litz RE (1985) Organ formation in Musa tissue cultures. Plant Physiol. 121: 123–130
Marroquin CG, Paduscheck C, Escalant JV & Teisson C (1993) Somatic embryogenesis and plant regeneration through cell suspensions in Musa acuminata. In Vitro Cell. Dev. Biol. 29P: 43–46
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497
Nadel BL, Altman A & Ziv M (1989) Regulation of somatic embryogenesis in celery cell suspensions. 1. Promoting effects of mannitol on somatic embryo development. PlantCell Tiss. Org. Cult.18: 181–189
Nickle TC & Yeung EC (1993) Failure to establish a functional shoot meristem may be a cause of conversion failure in somatic embryos of Daucus carota (Apiaceae). Am. J. Bot. 80: 1284–1291
Novak FJ, Afza R, Van Duren M, Perea-Dallos M., Conger BV & Xiaolang Taang (1989) Somatic embryogenesis and plant regeneration in suspension cultures of dessert (AA and AAA) and cooking (ABB) bananas (Musa spp.). Biotechnology 7: 154–159
Panis B, Van Wauwe A & Swennen R (1993) Plant regeneration through direct somatic embryogenesis from protoplasts of banana (Musa spp.) Plant Cell Rep. 12: 403–407
Sannasgala K, Dumortier FM & De Langhe EAL (1990) Somatic embryogenesis from Musa proliferating shoot tips: Histological details. In: In vitro Mutation Breeding of Bananas and Plantains 1. IAEA, Vienna (pp. 24–28).
Sass JE (1958) Botanical Microtechnique, 3rd ed. Iowa State College Press, Iowa
Schenk RU & Hildebrant AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204
Tisserat B & DeMason DA (1980) A hitological study of development of adventive embryos in organ cultures of Phoenix dactylifera L. Ann. Bot. 46: 445–472
Verhagen SA & Wann SR (1989) Norway spruce somatic embryogenesis high frequency initiation from light cultured mature embryos. Plant Cell Tiss. Org. Cult. 16: 103–111
Williams EG & Maheswaran G (1986) Somatic embryogenesis: Factors influencing coordinated behaviour of cells as an embryogenic group. Ann. Bot. 57: 443–462
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Lee, K., Zapata-Arias, F., Brunner, H. et al. Histology of somatic embryo initiation and organogenesis from rhizome explants of Musa spp.. Plant Cell, Tissue and Organ Culture 51, 1–8 (1997). https://doi.org/10.1023/A:1005985401756
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DOI: https://doi.org/10.1023/A:1005985401756