Abstract
In vitro-cultured adventitious bud clusters of Guazuma crinita Mart. were successfully cryopreserved by the one-step vitrification method. Small segments (1.0-1.5 mm3) cut from adventitious bud clusters were exposed to a cryoprotectant mix solution containing (w/v), 25 glycerol, 15 sucrose, 15 ethylene glycol, 13 dimethyl sulfoxide, and 2 polyethylene glycol, at 25 °C for 15-60 min prior to storage in liquid nitrogen. After rapid warming (37 °C), the segments were treated with woody plant medium containing 40 (w/v) sucrose for 20 min at 25 °C, and then transferred to recovery-growth medium. High survival rates (about 80) were achieved without any cold hardening and/or pre-culturing treatments, and about 30 of the surviving cryopreserved explants regenerated plants.
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Maruyama, E., Kinoshita, I., Ishii, K. et al. Germplasm conservation of Guazuma crinita, a useful tree in the Peru-Amazon, by the cryopreservation of in vitro-cultured multiple bud clusters. Plant Cell, Tissue and Organ Culture 48, 161–165 (1997). https://doi.org/10.1023/A:1005858324187
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DOI: https://doi.org/10.1023/A:1005858324187