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Embryo formation and regeneration in Triticum turgidum ssp. durum anther culture

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Abstract

Field grown Moroccan genotypes of durum wheat were tested for their capacity for androgenesis. The cultivar '1726' gave the best results with 25.37% embryos (embryos per 100 anthers) whereas '1715' was the least favorable with 3.17%. Spikes excised in early spring gave the best percentages of embryos. A chemical hybridizing agent (CHA) treatment of donor plants doubled the yield of embryos for almost all genotypes. A thermal pretreatment of spikes at 3 °C for 8 days improved androgenetic capacity. The C17 medium for embryo induction was consistently the best. Two media containing potato extract (BPTG and P2) gave intermediate results and N6 gave the lowest response. The highest percentage regeneration was obtained on C17 medium containing regulatory and amino substances, using embryos about 1 mm in diameter transferred to regeneration medium 21 days after their appearance. Regenerated plants were albino except for two green plants from cultivars 'Marzak' and '1658'.

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Authors and Affiliations

  1. Plant Physiology Laboratory, Science Faculty, Mohammed V University, B.P. 1014, Rabat, Morocco (*requests for offprints)

    Najia Saidi, Souad Cherkaoui, Averil Chlyah & Hassan Chlyah

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  1. Najia Saidi
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  2. Souad Cherkaoui
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  3. Averil Chlyah
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  4. Hassan Chlyah
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Saidi, N., Cherkaoui, S., Chlyah, A. et al. Embryo formation and regeneration in Triticum turgidum ssp. durum anther culture. Plant Cell, Tissue and Organ Culture 51, 27–33 (1997). https://doi.org/10.1023/A:1005765529154

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