Abstract
Different media were evaluated with anthers of five spring wheat (Triticum aestivum L.) genotypes for their ability to produce embryos and green plants in anther culture. Our first experiment showed that the addition of a combination of 19 amino acids significantly increased the number of embryos and green plants obtained. The mean number of green plants per 100 anthers for the two genotypes in this experiment, HY320 and B723, went from 28.2 without amino acids in the medium, to 46.7 with addition of amino acids. Our second experiment with the genotypes HY320, Wim and Laval-19 showed that liquid medium with Ficoll is more efficient for anther culture (9.9 green plants/100 anthers) than solid (0 green plants), gelationous media (2.5 green plants/100 anthers) or liquid medium with Membrane Rafts (0 green plants; Hoechst Celanese Corp.). Our third experiment revealed that the effect of replacement of sucrose by maltose varied with the genotype of the donor plant. Maltose partially inhibited the androgenesis of three responsive genotypes, HY320, Wim and Reliance (40.3 green plants/100 anthers instead of 43.9 with sucrose), while maltose significantly increased the androgenesis of the recalcitrant genotype Laval-19 (10.8 green plants/100 anthers instead of 5.4 with sucrose). An amino acid x maltose interaction was also observed. Amino acids without maltose increased androgenesis, but the addition of maltose to the amino acid-enriched medium eliminated this positive effect of the amino acids.
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References
Comeau A, Nadeau P, Plourde A, Simard R, Maës O, Kelly S, Harper L, Lettre J, Landry B & St-Pierre C-A (1992) Media for the in ovulo culture of proembryos of wheat and wheat-derived interspecific hybrids or haploids. Plant Sci. 81: 117–125
Guha S & Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204: 497–498
Henry Y & De Buyser J (1981) Float culture of wheat anthers. Theor. Appl. Genet. 60: 70–79
Hoekstra S, van Zijderveld MH, Louwerse JD, Heidekamp F & Van Der Mark F (1992) Anther and microspore culture of Hordeum vulgare cv. Igri. Plant Sci. 86: 89–96
Jones A & Petolino JF (1988) Effects of support medium on embryo and plant production from cultured anthers of soft-red winter wheat (Triticum aestivum L.). Plant Cell Tiss. Org. Cult. 12: 253–261
Kao K (1981) Plant formation from Barley anther cultures with Ficoll medium. Z. Pflanzenphysiol. 103: 437–443
Kasha KJ, Ziauddin A & Cho UH (1990) Haploids in cereal improvement: anther and microspore culture. In: Gustafson JP (Ed) Gene manipulation in plant improvement II. 19th Stadler Genetics Symposium (pp. 213–236). Plenum Press, New-York
Kuhlmann U & Foroughi-Wehr B (1989) Production of doubled haploid lines in frequencies sufficient for barley breeding programs. Plant Cell Rep. 8: 78–81
Last DJ & Brettell RIS (1990) Embryo yield in wheat anther culture is influenced by the choice of sugar in the culture medium. Plant Cell Rep. 9: 14–16
Lettre JJ, Kelly SL, Marsolais AA & Kasha KJ (1990) Wheat anther culture using liquid media. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry. Vol 13 (pp. 168–180). Springer-Verlag, Berlin Heidelberg
Maheswari SC, Tyagi AK & Malhotra K (1980) Induction of haploidy from pollen grains in angiosperms-the current status. Theor. Appl. Genet. 58: 193–206
Marsolais AA & Kasha KJ (1985) Callus induction from barley microspores. The role of sucrose and auxin in a barley anther culture medium. Can. J. Bot. 63: 2209–2212
Marsolais AA, Wheatley WG & Kasha KJ (1986) Progress in wheat and barley haploid induction using anther culture. N.Z. Agronomy. Special Publication No. 5: 340–343
Muyan Z, Abing X, Miaobao Y, Chunnong H, Zhilong Y, Linji W & Jianjun Y (1990) Effects of amino acids on callus differentiation in barley anther culture. Plant Cell Tiss. Org. Cult. 22: 201–204
Nitsch C (1974) Pollen culture: a new technique for mass production of haploids and homozygous plants. In: Kasha KJ (Ed) Haploids in Higher Plants: Advances and Potential. Proc. of the first symp. (pp. 123–135). University of Guelph, Ontario, Canada
Orshinsky BR, McGregor LJ, Johnson GIE, Hucl P & Kartha KK (1990) Improved embryoid induction and green shoot regeneration from wheat anthers cultured in medium with maltose. Plant Cell Rep. 9: 365–369
Picard E & De Buyser J (1977) High production of embryoids in anther culture of pollen derived homozygous spring wheats. Ann. Amélior. Plant. 24: 483–488
Roberts-Oehlschlager SL, Dunwell JM & Faulks R (1990) Change in sugar content of barley anthers during culture on different carbohydrates. Plant Cell Tiss. Org. Cult. 22: 77–85
Tiainen T (1992) The influence of culture conditions on anther culture response of commercial varieties of Solanum tuberosum L. Plant Cell Tiss. Org. Cult. 30: 211–219
Xu ZH & Sunderland N (1981) Glutamine, inositol and conditioning factor in the production of barley pollen callus in vitro. Plant Sci. Lett. 23: 161–168
Zhou H, Zheng Y & Konzak CF (1991) Osmotic potential of media affecting green plant percentage in wheat anther culture. Plant Cell Rep. 10 (2): 63–66
Zhou H, Ball ST & Konzak CF (1992) Functional properties of Ficoll and their influence on anther culture responses of wheat. Plant Cell Tiss. Org. Cult. 30: 77–83
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Trottier, MC., Collin, J. & Comeau, A. Comparison of media for their aptitude in wheat anther culture. Plant Cell Tiss Organ Cult 35, 59–67 (1993). https://doi.org/10.1007/BF00043940
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DOI: https://doi.org/10.1007/BF00043940