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Variants of Digitalis obscura from irradiated shoot tips

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Abstract

Shoot tips excised from genotype T4 of Digitalis obscura were exposed to gamma rays (20–100 Gy). Radiosensitivity was assessed and LD50 determined was about 60 Gy. The effect of the herbicide amitrole on shoot-tip culture was investigated, efficient bleaching was obtained with 10 mg/l amitrole. Shoot tips mutagenized (20–40 Gy) were cultured on several selective amitrole-containing media, but none of them permitted prolonged survival of the green shoots formed. Ploidy level of the plantlets developed was analysed by flow cytometry 8 and 18 months after culture establishment. Variations detected corresponded to aneuploid changes and increased in parallel with the gamma radiation dose. Plantlets developed from irradiated shoot tips presented a high variability in their cardenolide production (878 to 3291 μg/g d.w.), including variants with similar or even higher productivities than the native T4 plant.

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Authors and Affiliations

  1. Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, Av. V.A. Estellés s/n, 46100, Burjassot (Valencia), Spain

    Pedro Pérez-Bermúdez

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  1. Isabel Gavidia
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  2. Pedro Pérez-Bermúdez
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Gavidia, I., Pérez-Bermúdez, P. Variants of Digitalis obscura from irradiated shoot tips. Euphytica 110, 153–159 (1999). https://doi.org/10.1023/A:1003768300465

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