Summary
By using mercury(II)-chloride (HgCl2) and Dl-6-fluorotryptophan (6FT) as positive selection conditions we were able to show that N-methyl-N′-nitro-N-nitrosoguanidine (NG) is an effective mutagen for Petunia hybrida suspension cells.
A number of the 205 calli resistant to HgCl2 and 17 calli resistant to 6FT were isolated. The highest mutation frequency was 1.0 x 10−5 and 2.0 x 10−6 for HgCl2 and 6FT, respectively. A preliminary characterization of the mutants is presented.
A significant increase in the number of drug-resistant calli was only obtained at NG-concentrations (5–40 μg/ml) that had no observable effect on the survival of the mutagenized cultures.
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Literature
Murashige, T.; Skoog, F.: A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15, 473–497 (1962)
Nishi, A.; Yoshida, A.; Mori, M; Sugano, N.: Isolation of variant carrot cell lines with altered pigmentation. Phytochemistry 13, 1653–1656 (1974)
Sung, Z.R.: Auxin-independent growth in a carrot line resistant to Dl-5-methyltryptophan. Plant Physiol. Suppl. 56, 37 (1975)
Sung, R.: Mutagenesis of cultures plant cells. Genetics 84, 51–57 (1976)
Vasil, J.K.; Nitsch, C.: Experimental production of pollen haploids and their uses. Z. Pflanzenphysiol. 76, 191–212 (1975)
Widholm, J.M.: The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 47, 189–194 (1972a)
Widholm, J.M.: Antranilate synthetase from 5-methyltryptophan susceptible and -resistant cultured Daucus carota cells. Biochem. Biophys. Acta 279, 48–57 (1972b)
Widholm, J.M.: Selection and characterization of cultured carrot and tobacco cells resistant to lysine, methionine, and proline analogs. Can. J. Bot. 54, 1523–1529 (1976)
Widholm, J.M.: Selection and characterization of biochemical mutants. In: Plant tissue culture and its bio-technological applications (eds. Barz, W.; Reinhard, E.; Zenk, M.H.), pp. 112–122. Berlin-Heidelberg-New York: Springer 1977a
Widholm, J.M.: Isolation of biochemical mutants of cultured plant cells. In: Molecular genetic modification of eukaryotes (eds. Rubenstein, J.; Phillips, R.L.; Green, C.E.; Desnick, R.), pp. 57–65. New York: Academic Press 1977b
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Communicated by G. Wenzel
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Colijn, C.M., Kool, A.J. & Nijkamp, H.J.J. An effective chemical mutagenesis procedure for Petunia hybrida cell suspension cultures. Theoret. Appl. Genetics 55, 101–106 (1979). https://doi.org/10.1007/BF00295434
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DOI: https://doi.org/10.1007/BF00295434