Abstract
The morphogenic response of somatic (leaf and petiole) and de-differentiated tissue (callus) of two blackberry (Rubus fruticosus) and one raspberry (Rubus idaeus) cultivars have been studied in vitro. With the aim to induce regeneration the effect of two sets of plant growth regulator (PGR) combinations (high cytokinin/auxin ratios and high auxin/cytokinin ratios) in Murashige and Skoog basal medium, were analysed. The three cultivars were characterised by a qualitatively different morphogenic response to the PGR combinations. Raspberry adventitious shoot regeneration from somatic tissue was improved by the 6-benzylaminopurine (BAP)/indol-3-butyric acid (IBA) combinations. On the contrary, shoot regeneration of both blackberry cultivars was reduced by high concentrations of BAP and completely inhibited by BAP/IBA combination. Media supplemented with high auxin/cytokinin ratios promoted callus production and root differentiation according to genotype and type of auxin. All the genotypes responded to media supplemented with IBA. 2,4-dichlorophenoxyacetic acid induced good callus formation in blackberry, but was toxic to raspberry. Indirect shoot formation was observed only in callus of blackberry cultivar Hull Thornless cultivated on medium with 10 µM BAP, the same concentration able to trigger efficient direct shoot regeneration from leaf explants of the same cultivar.
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Berardi, G., Ancherani, M., Rosati, P.: Estimation of callus growth from petals of GF677 peach × almond rootstock.-G. bot. ital. 127: 1170–1172, 1993.
Borejsza-Wysocki, W., Hrazdin, W.: Establishment of callus and cell suspension cultures of raspberry (Rubus idaeus cv. Royalty).-Plant Cell Tissue Organ Cult. 37: 213–216, 1994.
Cousineau, J.C., Donnelly, D.J.: Adventitious shoot regeneration from leaf explants of tissue cultured and greenhouse-grown raspberry.-Plant Cell Tissue Organ Cult. 27: 249–255, 1991.
Estruch, J.J., Prinsen, E., Onckelen, H.V., Schell, J., Spena, A.: Viviparous leaves produced by somatic activation of an inactive cytokinin-synthesizing gene.-Science 254: 1364–1367, 1991.
Fiola, J.A., Hassan, M.A., Swarth, H.J., McNicols, R.: Effect of thidiazuron, light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledon and leaves.-Plant Cell Tissue Organ Cult. 20: 223–228, 1990.
Firoozabady, E., DeBoer, D.L.: Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.).-In vitro Cell Develop. Biol. 29: 166–173, 1993.
Hicks, G.S.: Patterns of organ development in plant tissue culture and the problem of organ determination.-Bot. Rev. 46: 1–23, 1980.
King, P.J.: Plant hormone mutants.-Trends Genet. 4: 157–162, 1988.
Klee, H., Estelle, M.: Molecular genetic approaches to plant hormone biology.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 529–521, 1991.
Iasi, L., Infante, R., Ochatt, S.J.: Protoplast isolation and culture of blackberry and raspberry cultivars (Rubus spp.).-J. Genet. Breeding 48: 149–154, 1994.
Lienart, Y., Gautier, C., Driguez, H.: Isolation from Rubus cell-suspension cultures of a lectin specific for glucosamine oligomers.-Planta 184: 8–13, 1991a.
Lienart, Y., Gautier, C., Driguez, H.: Silica-bound N-acetylglucosamyl residues elicit laminarinase activity in Rubus protoplast.-Plant Sci. 77: 41–45, 1991b.
McNicol, R.J., Graham, J.: In vitro regeneration of Rubus from leaf and stem segments.-Plant Cell Tissue Organ Cult. 21: 45–50, 1990.
Merkle, S.A., Parrot, W.A., Williams, E.G.: Applications of somatic embryogenesis and embryo cloning.-In: Bhojawani, S.S. (ed.): Plant Tissue Culture: Applications and Limitations. Pp. 67–101. Elsevier, Dublin 1990.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissues cultures.-Physiol. Plant. 15: 473–497, 1962.
Owens, L.D., Smigocki, A.C.: Transfer of phytohormone genes to induce morphogenesis in plants.-Adv. Cell Cult. 7: 183–199, 1988.
Owens y De Novoa, C., Conner, A.J.: Comparison of in vitro shoot regeneration protocols from Rubus leaf explants.-New Zeal. J. Crop Hort. Sci. 20: 471–476, 1992.
Shell, J., Van Montagu, M., Holsters, M., Hernalsteens, J.P., Dhaese, P., De Greve, H., Leemans, J., Joos, H., Inze, D., Willmitzer, L., Otten, L., Wostemeyer, A., Schroeder, J.: Plant cells transformed by modified Ti plasmids: A model system to study plant development.-In: Jaenicke, L. (ed.): Biochemistry of Differentiation and Morphogenesis. Pp. 65–73. Springer-Verlag, Berlin 1982.
Skoog, F., Miller, C.O.: Chemical regulation of growth and organ formation in plants tissues cultured in vitro.-Symp. Soc. Exp. Biol. 11: 118–130, 1957.
Smigocki, A.C., Owens, L.D.: Cytokinin-to-auxin ratios and morphology of shoots and tissue transformed by chimeric isopentenyl transferase gene.-Plant Physiol. 91: 808–811, 1989.
Spano, L., Mariotti, D., Cardarelli, M., Branca, C., Constantino, P.: Morphogenesis and auxin sensitivity of transgenic tobacco with different complements of Ri Ti-DNA.-Plant Physiol. 87: 479–483, 1988.
Swartz, H.J., Bors, R., Mohamed, F., Naess, S.K.: The effect of in vitro pretreatments on subsequent organogenesis from excised Rubus and Malus leaves.-Plant Cell Tissue Organ Cult. 21: 179–184, 1990.
Taylor, R.J., Secor, G.A.: Average tissue diameter as non-destructive determinant of potato protoplast-derived callus growth.-Environ. exp. Bot. 32: 43–48, 1992.
Thorpe, T.A.: Morphogenesis and regeneration.-In: Vasil, I.K., Thorpe, T.A. (ed.): Plant Cell and Tissue Culture. Pp. 17–36. Kluwer Academic Publishers, Dordrecht 1994.
Treavas, A.: How do plant growth substances work.-Plant Cell Environ 4: 202–228, 1981.
Turk, B.A., Swartz, H.J., Zimmerman, R.H.: Adventitious shoot regeneration from in vitro-cultured leaves of Rubus genotypes.-Plant Cell Tissue Organ Cult. 38: 11–17, 1994.
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Mezzetti, B., Savini, G., Carnevali, F. et al. Plant genotype and growth regulators interaction affecting in vitro morphogenesis of blackberry and raspberry. Biologia Plantarum 39, 139–150 (1997). https://doi.org/10.1023/A:1000381612029
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DOI: https://doi.org/10.1023/A:1000381612029