Abstract
A new and rapid procedure has been developed for the isolation of the bacteriochlorophyll a-containing Fenna—Matthews—Olson (FMO)-protein from green sulfur bacteria. Polyclonal antibodies raised against the FMO-protein of Chlorobium (Chl.) tepidum were employed in the preparation of an antibody column utilizing immobilized protein A as the matrix. The antibody column afforded essentially a one-step purification process, resulting in preparations that were free from contaminating pigments and proteins. This was evidenced by absorption spectroscopy, SDS—PAGE, and fluorescence emission.
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Zhou W (1995) Ph.D Thesis, Arizona State University, Tempe, AZ
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Hu, D.D., Blankenship, R.E. Rapid one-step purification of the BChl-a containing FMO-protein from the green sulfur bacterium Chlorobium tepidum using a high efficiency immunomatrix. Photosynthesis Research 71, 149–154 (2002). https://doi.org/10.1023/A:1014907731595
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DOI: https://doi.org/10.1023/A:1014907731595