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Defence Responses Against TNV Infection Induced by Galactoglucomannan-derived Oligosaccharides in Cucumber Cells

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Abstract

Galactoglucomannan-derived oligosaccharides (GGMOs) showing biological activity in growth, morphogenesis and cell viability were tested in a host pathogen interaction. As a model system, cucumber (Cucumis sativus L. cv. Laura) reacting hypersensitively to tobacco necrosis virus (TNV) was used. The defence reactions were dependent on the degree of polymerisation and concentration of oligosaccharides, as well as on the time of application of virus to plant cotyledons. Disease symptoms were inhibited by 60–75%. The average number of lesions per cotyledon was significantly decreased when oligosaccharides were used simultaneously or 24 h prior to virus inoculation. Significant changes in peroxidase, beta-glucanase and chitinase activities accompanied the defence reaction. It can be concluded that oligosaccharides derived from spruce galactoglucomannan induce non-specific resistance to local viral infection in plants. GGMOs probably act as inhibitors of the virus infection, rather than inhibitors of direct virus multiplication.

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References

  • Aldington S, McDougall GJ and Fry SC (1991) Structure-activity relationships of biologically active oligosaccharides. Plant Cell Environment 14: 625–636

    Google Scholar 

  • Albersheim P, Darvill AG, Augur Ch, Cheong JJ, Eberhard S, Hahn MG, Marfá V, Mohnen D, O'Neill MA, Spiro MD and York WS (1992) Oligosaccharins: oligosaccharide regulatory molecules. Accounts of Chemical Research 25: 77–83

    Google Scholar 

  • Auxtová O, Lišková D, Kákoniová D, Kuba?ková M, Karácsonyi Š and Bilisics L (1995) Effect of galactoglucomannan-derived oligosaccharides on elongation growth of pea and spruce stem segments stimulated by auxin. Planta 196: 420–424

    Google Scholar 

  • Auxtová-šamajová O, Lišková D, Kákoniová D, Kuba?ková M, Karácsonyi Š and Bilisics L (1996) Inhibition of auxin stimulated short-term elongation growth of pea stem segments by galactoglucomannan-derived oligosaccharides. Journal of Plant Physiology 147: 611–613

    Google Scholar 

  • Bawden FC (1954) Inhibitors of plant viruses. Advances in Virus Research 2: 31–57

    Google Scholar 

  • Bellincampi D, Salvi G, De Lorenzo G, Cervone F, Marfá V, Eberhard S, Darvill A and Albersheim P (1993) Oligogalacturonides inhibit the formation of roots on tobacco explants. Plant Journal 4: 207–213

    Google Scholar 

  • Benhamou N, Joosten MHAJ and De Wit PJGM (1990) Subcellular localization of chitinase and of its potential substrate in tomato root tissues infected by Fusarium oxysporum f.sp. radicis-lycopersici. Plant Physiology 92: 1108–1120

    Google Scholar 

  • Boller T, Gehri A, Mauch F and Vögeli U (1983) Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function. Planta 157: 22–31

    Google Scholar 

  • Bradford N (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Analytical Biochemistry 72: 248–254

    Google Scholar 

  • Capek P, Bilisics L, Kuba?ková M, Alföldi J, Vojtaššák J, Lišková D, Kákoniová D and Sadlo?ová K (1998) Isolation and structural characterization of the mannose-rich polysaccharide from the wood of Picea abies L. Karst. 3rd International Symposium on Wood Structure and Properties, Zvolen 25–27 August 1998, Slovakia

  • Cervone F, Hahn MG, De Lorenzo G, Darvill A and Albersheim P (1989) Host pathogen interactions. XXXIII. A plant protein converts a fungal pathogenesis factor into an elicitor of plant defense responses. Plant Physiology 90: 542–548

    Google Scholar 

  • Conrath U, Domard A and Kauss H (1989) Chitosan-elicited synthesis of callose and of coumarin derivatives in parsley cell suspension cultures. Plant Cell Report 8: 152–155

    Google Scholar 

  • Coté F and Hahn MG (1994) Oligosaccharins: structures and signal transduction. Plant Molecular Biology 26: 1379–1411

    Google Scholar 

  • Darvill AG, Augur C, Bergmann C, Carlson RW, Cheong JJ, Eberhard S, Hahn MG, Lo VM, Marfá V, Meyer B, Mohnen D, O'Neill MA, Spiro MD, van Halbeek H, Zork WS and Albersheim P (1992) Oligosaccharins-oligosaccharides that regulate growth, development and defense responses in plants. Glycobiology 2: 181–198

    Google Scholar 

  • Dubois M, Gilles KA, Hamilton KJ, Rebers PA and Smith E (1959) Colorimetric methods for determination of sugars and related substances. Analytical Biochemistry 28: 350–356

    Google Scholar 

  • Fritig B, Kaufmann S, Dumas B, Geoffroy P, Kopp M and Legrand M (1987) Mechanism of the hypersensitivity reaction of plants. In: Evered D and Harnett S (eds) Plant Resistance to Viruses, Ciba Foundation Symposium 133 (pp 92–112) John Wiley & Sons, Chichester, UK

    Google Scholar 

  • Fri? F and Fuchs WH (1970) Veränderungen der Aktivität Einiger Enzyme imWeizenblatt in Abhängigkeit von Puccinia graminis tritici. Phytopathologische Zeitschrift 7: 161–174

    Google Scholar 

  • Fry SC (1986) Cross-linking of matrix polymers in the growing cell walls of angiosperms. Annual Review of Plant Physiology 37: 165

    Google Scholar 

  • Fry (1996) Oligosaccharin mutants. Trends in Plant Science-Research News 1: 326–328

    Google Scholar 

  • Graham MY and Graham TL (1991) Rapid accumulation of anionic peroxidases in soybean cotyledon tissues following treatment with Phytophthora megasperma f.sp. glycinea wall glucan. Plant Physiology 97: 1445–1455

    Google Scholar 

  • Hammerschmidt R, Nuckles EM and Kuc J (1982) Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiological Plant Pathology 20: 73–82

    Google Scholar 

  • Hirai T (1977) Action of antiviral agents. In: Horsfall JG and Cowling EB (eds) Plant Disease: An Advanced Treatise, Vol 1 (pp 258–305) Academic Press, New York

    Google Scholar 

  • Hughes RK and Dickerson AG (1991) Modulation of elicitorinduced chitinase and ?-1,3–glucanase activity by hormones in Phaseolus vulgaris. Plant Cell Physiology 32: 853–861

    Google Scholar 

  • Ji C and Kuc J (1996) Antifungal activity of cucumber ?-1,3–glucanase and chitinase. Physiological and Molecular Plant Pathology 49: 257–265

    Google Scholar 

  • Karácsonyi Š, Ková?ik V and Kákoniová D (1996) Isolation and characterisation of cell wall polysaccharides from Picea abies L. Cellulose Chemistry and Technology 30: 359–370

    Google Scholar 

  • Klement Z (1965) Method of obtaining fluid from the intercellular spaces of foliage and the fluid merit as substrate for phytobacterial pathogens. Phytopathology 55: 1003–1034

    Google Scholar 

  • Kopp M, Rouster J, Fritig B, Darvill A and Albersheim P (1989) Host-pathogen interaction. XXXII. A fungal glucan preparation protect Nicotianae against infection by viruses. Plant Physiology 90: 208–216

    Google Scholar 

  • Lagrimini LM and Rothstein S (1987) Tissue specificity of tobacco peroxidase isozymes and their induction by wounding and tobacco mosaic virus infection. Plant Physiology 84: 438–442

    Google Scholar 

  • Lesney MS (1990) Effect of ‘elicitors’ on extracellular peroxidase activity in suspension-cultured slash pine (Pinus elliottii Engelm.). Plant Cell Tissue Organ Culture 20: 173–175

    Google Scholar 

  • Lišková D, Auxtová O, Kákoniová D, Kuba?ková M, Karácsonyi Š and Bilisics L (1995) Biological activity of galactoglucomannan-derived oligosaccharides. Planta 196: 425–429

    Google Scholar 

  • Marfá V, Gollin DJ, Eberhard S, Mohnen D, Darvill A and Albersheim P (1991) Oligogalacturonides are able to induce flowers on tobacco explants. Plant Journal 1: 217–225

    Google Scholar 

  • Masuta C, Van den Bulcke M, Bauw G, Van Montagu M and Caplan AB (1991) Differential effects of elicitors on the viability of rice suspension cells. Plant Physiology 97: 619–629

    Google Scholar 

  • Mathieu Y, Kurkdjian A, Xia H, Guern J, Koller A, Spiro MD, O'Neil M, Albersheim P and Darvill A (1991) Membrane responses induced by oligogalacturonides in suspensioncultured tobacco cells. Plant Journal 1: 333–343

    Google Scholar 

  • Mills AR and Timell TE (1963) Constitution of three hemicelluloses from the wood of Engelmann spruce (Picea engelmanni). Canadian Journal of Chemistry 41: 1389–1395

    Google Scholar 

  • Mohan R and Kolattukudy PE (1990) Differential activation of expression of a suberinization-associated anionic peroxidase gene in near-isogenic resistant and susceptible tomato lines by elicitors of Verticillium albo-atrum. Plant Physiology 92: 276–280

    Google Scholar 

  • Moore AE and Stone BA (1972) Effect of infection of TMV and other viruses on the level of a ?-1,3–glucan hydrolase in leaves of Nicotiana glutinosa. Virology 50: 791–798

    Google Scholar 

  • Peng M and Kuc J (1992) Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf discs. Phytopathology 82: 696–699

    Google Scholar 

  • Repka V and Slováková L' (1994) Purification, characterisation and accumulation of three virus-induced cucumber peroxidases. Biologia Plantarum 36: 121–132

    Google Scholar 

  • Singh RP, Wood FA and Hodgson WA (1970) The nature of virus inhibition by a polysaccharide from Phytophthora infestans. Phytopathology 60: 1566–1569

    Google Scholar 

  • Shapira J (1969) Identification of trifluoroacetyl polyols by gas chromatography. Nature 222: 792–793

    Google Scholar 

  • Slováková L', Šubiková V and Farkaš V (1994) Influence of xyloglucan oligosaccharides on some enzymes involved in the hypersensitive reaction to TNV (tobacco necrosis virus) of cucumber cotyledons. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 101: 278–285

    Google Scholar 

  • Šubiková V (1994) Virus diseases of forest trees. Acta Horticulturae 337: 367–371

    Google Scholar 

  • Šubiková V, Slováková L' and Farkaš V (1994) Inhibition of tobacco necrosis virus infection by xyloglucan fragments. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 101: 128–131

    Google Scholar 

  • Tuzun S, Rao MN, Vogeli U, Schradl ChL and Kuc J (1989) Induced systemic resistance to blue mould: early induction and accumulation of ?-1,3–glucanases, chitinases and other pathogenesis related proteins (b-proteins) in immunized tobacco. Phytopathology 79: 979–983

    Google Scholar 

  • Van den Blucke M, Bauw G, de Ricke R, Castresana C, Van Montagu M and Vandekerckhove J (1990) The role of vacuolar and secreted pathogenesis-related ?(1–3)glucanases and chitinases in the defence response of plants. Bulletin Société Bottanique de France 137, Actualité Botanique (3/4): 53–63

    Google Scholar 

  • Wirth SJ and Wolf GA (1990) Dye-labelled substrates for the assay and detection of chitinase and lysozyme activity. Journal of Microbiological Methods 12: 197–205

    Google Scholar 

  • Wood FA, Singh RP and Hodgson WA (1971) Characterization of a virus-inhibiting polysaccharide from Phytophthora infestans. Phytopathology 61: 1006–1009

    Google Scholar 

  • Ye XS, Pan SQ and Kuc J (1990) Activity, isozyme pattern, and cellular localization of peroxidase as related to systemic resistance of tobacco to blue mould (Peronospora tabacina) and to tobacco mosaic virus. Physiological Biochemistry 80: 1295–1299

    Google Scholar 

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Authors and Affiliations

  1. Institute of Experimental Phytopathology and Entomology, Slovak Academy of Sciences, Nádražná 52, 900 28, Ivánka pri Dunaji, Slovakia

    L'udmila Slováková

  2. Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 842 38, Bratislava, Slovakia;

    Desana Lišková

  3. Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 842 38, Bratislava, Slovakia

    Peter Capek, Daniela Kákoniová & Štefan Karácsonyi

Authors
  1. L'udmila Slováková
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  2. Desana Lišková
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  3. Peter Capek
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  4. Marta Kubačková
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  5. Daniela Kákoniová
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  6. Štefan Karácsonyi
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Slováková, L., Lišková, D., Capek, P. et al. Defence Responses Against TNV Infection Induced by Galactoglucomannan-derived Oligosaccharides in Cucumber Cells. European Journal of Plant Pathology 106, 543–553 (2000). https://doi.org/10.1023/A:1008722318178

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