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Oligosaccharide elicitor prepared from Salecan triggers the defense responses of Arabidopsis thaliana Col0 against Botrytis cinerea infection

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Abstract

Oligosaccharides from the water-soluble β-glucan, Salecan, were investigated to evaluate the activation effect on the defense responses of Arabidopsis thaliana Col0. Salecan oligosaccharides (ScOs, DP 5–10) were prepared at first by acid hydrolysis and gel filtration chromatography and then employed for foliar spray on Arabidopsis seedlings and plants. After ScOs treatment, increase of hydrogen peroxide was histologically and analytically detected in leaves. Transcription levels of several indicator genes which correspond to the signaling pathways and pathogenesis-related proteins were up-regulated at different time by means of quantitative RT-PCR analysis. Importantly, the sequential activation of salicylic acid-mediated and jasmonate-mediated signaling pathway was observed in leaves. Furthermore, pot test and antimicrobial test gave the fact that pretreatment with ScOs restrained the leaf lesions caused by infection of Botrytis cinerea Bc0510 via the enhancement of defense responses of A. thaliana. In conclusion, Salecan oligosaccharides serve as an elicitor which can be used for biological control of plant pathogen.

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References

  • Bosch M, Berger S, Schaller A, Stintzi A (2014) Jasmonate-dependent induction of polyphenol oxidase activity in tomato foliage is important for defense against Spodoptera exigua but not against Manduca sexta. BMC Plant Biol 14:257. doi:10.1186/s12870-014-0257-8

    Article  Google Scholar 

  • Chen P et al (2012) Protective effects of salecan against carbon tetrachloride-induced acute liver injury in mice. J Appl Toxicol 32:796–803. doi:10.1002/jat.1694

    Article  CAS  Google Scholar 

  • Cheong JJ, Hahn MG (1991) A specific, high-affinity binding site for the hepta-beta-glucoside elicitor exists in soybean membranes. Plant Cell 3:137–147

    CAS  Google Scholar 

  • Denance N, Sanchez-Vallet A, Goffner D, Molina A (2013) Disease resistance or growth: the role of plant hormones in balancing immune responses and fitness costs. Front Plant Sci 4:155. doi:10.3389/fpls.2013.00155

    Article  Google Scholar 

  • Dixon RA, Achnine L, Kota P, Liu CJ, Reddy MSS, Wang LJ (2002) The phenylpropanoid pathway and plant defence: a genomics perspective. Mol Plant Pathol 3:371–390. doi:10.1046/j.1364-3703.2002.00131.x

    Article  CAS  Google Scholar 

  • Dodds PN, Rathjen JP (2010) Plant immunity: towards an integrated view of plant-pathogen interactions. Nat Rev Genet 11:539–548. doi:10.1038/nrg2812

    Article  CAS  Google Scholar 

  • Fu Y, Yin H, Wang W, Wang M, Zhang H, Zhao X, Du Y (2011) b-1,3-Glucan with different degree of polymerization induced different defense responses in tobacco. Carbohydr Polym 86:774–782. doi:10.1016/j.carbpol.2011.05.022

    Article  CAS  Google Scholar 

  • Gorska-Czekaj M, Borucki W (2013) A correlative study of hydrogen peroxide accumulation after mercury or copper treatment observed in root nodules of Medicago truncatula under light, confocal and electron microscopy. Micron 52–53:24–32. doi:10.1016/j.micron.2013.07.007

    Article  Google Scholar 

  • Hael-Conrad V, Abou-Mansour E, Diaz-Ricci JC, Metraux JP, Serrano M (2015) The novel elicitor AsES triggers a defense response against Botrytis cinerea in Arabidopsis thaliana. Plant Sci 241:120–127. doi:10.1016/j.plantsci.2015.09.025

    Article  CAS  Google Scholar 

  • Han S, Wang C-w, Wang W-l, Jiang J (2014) Mitogen-activated protein kinase 6 controls root growth in Arabidopsis by modulating Ca2+-based Na+ flux in root cell under salt stress. J Plant Physiol 171:26–34. doi:10.1016/j.jplph.2013.09.023

    Article  CAS  Google Scholar 

  • Henriquez MA, Wolski EA, Molina OI, Adam LR, Andreu AB, Daayf F (2012) Effects of glucans and eicosapentaenoic acid on differential regulation of phenylpropanoid and mevalonic pathways during potato response to Phytophthora infestans. Plant Physiol Biochem 60:119–128. doi:10.1016/j.plaphy.2012.07.027

    Article  CAS  Google Scholar 

  • Jones JDG, Dangl JL (2006) The plant immune system. Nature 444:323–329. doi:10.1038/nature05286

    Article  CAS  Google Scholar 

  • Klarzynski O, Plesse B, Joubert JM, Yvin JC, Kopp M, Kloareg B, Fritig B (2000) Linear b-1,3 glucans are elicitors of defense responses in tobacco. Plant Physiol 124:1027–1037. doi:10.1104/pp.124.3.1027

    Article  CAS  Google Scholar 

  • Lee HY, Back K (2016) Mitogen-activated protein kinase pathways are required for melatonin-mediated defense responses in plants. J Pineal Res 60:327–335. doi:10.1111/jpi.12314

    Article  CAS  Google Scholar 

  • Lee J, Eschen-Lippold L, Lassowskat I, Boettcher C, Scheel D (2015) Cellular reprogramming through mitogen-activated protein kinases. Front Plant Sci 6:940. doi:10.3389/fpls.2015.00940

    Google Scholar 

  • Li J, Zhu L, Zheng Z-Y, Zhan X-B, Lin C-C, Zong Y, Li W-J (2013) A new effective process for production of curdlan oligosaccharides based on alkali-neutralization treatment and acid hydrolysis of curdlan particles in water suspension. Appl Microbiol Biotechnol 97:8495–8503. doi:10.1007/s00253-013-5125-1

    Article  CAS  Google Scholar 

  • Li J, Zhu L, Lu G, Zhan X-B, Lin C-C, Zheng Z-Y (2014) Curdlan β-1,3-glucooligosaccharides induce the defense responses against Phytophthora infestans infection of potato (Solanum tuberosum L. cv. McCain G1) leaf cells. PLoS ONE 9:e0097197. doi:10.1371/journal.pone.0097197

    Google Scholar 

  • Lopez-Bucio JS et al (2014) Arabidopsis thaliana mitogen-activated protein kinase 6 is involved in seed formation and modulation of primary and lateral root development. J Exp Bot 65:169–183. doi:10.1093/jxb/ert368

    Article  CAS  Google Scholar 

  • Nie S, Xu H (2016) Riboflavin-induced disease resistance requires the mitogen-activated protein kinases 3 and 6 in Arabidopsis thaliana. PLoS ONE 11:e0153175. doi:10.1371/journal.pone.0153175

    Article  Google Scholar 

  • Nováková M, Šašek V, Dobrev PI, Valentová O, Burketová L (2014) Plant hormones in defense response of Brassica napus to Sclerotinia sclerotiorum: Reassessing the role of salicylic acid in the interaction with a necrotroph. Plant Physiol Biochem 80:308–317. doi:10.1016/j.plaphy.2014.04.019

    Article  Google Scholar 

  • Pieterse CMJ, Leon-Reyes A, Van der Ent S, Van Wees SCM (2009) Networking by small-molecule hormones in plant immunity. Nat Chem Biol 5:308–316. doi:10.1038/nchembio.164

    Article  CAS  Google Scholar 

  • Tang F, Zhao W-L, Gao X-W (2013) Communication between plants: induced resistance in poplar seedlings following herbivore infestation, mechanical wounding, and volatile treatment of the neighbors. Entomol Exp Appl 149:110–117. doi:10.1111/eea.12114

    CAS  Google Scholar 

  • Thaler JS, Humphrey PT, Whiteman NK (2012) Evolution of jasmonate and salicylate signal crosstalk. Trends Plant Sci 17:260–270. doi:10.1016/j.tplants.2012.02.010

    Article  CAS  Google Scholar 

  • Trouvelot S et al (2014) Carbohydrates in plant immunity and plant protection: roles and potential application as foliar sprays. Front Plant Sci 5:592. doi:10.3389/fpls.2014.00592

    Article  Google Scholar 

  • Tsuda K, Sato M, Stoddard T, Glazebrook J, Katagiri F (2009) Network properties of robust immunity in plants. PLoS Genet 5:e1000772

    Article  Google Scholar 

  • van Kan JAL (2006) Licensed to kill: the lifestyle of a necrotrophic plant pathogen. Trends Plant Sci 11:247–253. doi:10.1016/j.tplants.2006.03.005

    Article  Google Scholar 

  • Vicente J, Cascon T, Vicedo B, Garcia-Agustin P, Hamberg M, Castresana C (2012) Role of 9-lipoxygenase and a-dioxygenase oxylipin pathways as modulators of local and systemic defense. Mol Plant 5:914–928. doi:10.1093/mp/ssr105

    Article  CAS  Google Scholar 

  • Wang Z, Tan X, Zhang Z, Gu S, Li G, Shi H (2012) Defense to Sclerotinia sclerotiorum in oilseed rape is associated with the sequential activations of salicylic acid signaling and jasmonic acid signaling. Plant Sci 184:75–82. doi:10.1016/j.plantsci.2011.12.013

    Article  CAS  Google Scholar 

  • Wang C, Ding Y, Yao J, Zhang Y, Sun Y, Colee J, Mou Z (2015) Arabidopsis elongator subunit 2 positively contributes to resistance to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola. Plant J 83:1019–1033. doi:10.1111/tpj.12946

    Article  CAS  Google Scholar 

  • Xiu A, Kong Y, Zhou M, Zhu B, Wang S, Zhang J (2010) The chemical and digestive properties of a soluble glucan from Agrobacterium sp. ZX09. Carbohydr Polym 82:623–628. doi:10.1016/j.carbpol.2010.05.027

    Article  CAS  Google Scholar 

  • Zhang X, Wang C, Zhang Y, Sun Y, Mou Z (2012) The Arabidopsis mediator complex subunit16 positively regulates salicylate-mediated systemic acquired resistance and jasmonate/ethylene-induced defense pathways. Plant Cell 24:4294–4309. doi:10.1105/tpc.112.103317

    Article  CAS  Google Scholar 

  • Zhao X, She X, Du Y, Liang X (2007) Induction of antiviral resistance and stimulary effect by oligochitosan in tobacco. Pestic Biochem Physiol 87:78–84. doi:10.1016/j.pestbp.2006.06.006

    Article  CAS  Google Scholar 

  • Zhou M et al (2014) Supplementation of the diet with Salecan attenuates the symptoms of colitis induced by dextran sulphate sodium in mice. Br J Nutr 111:1822–1829. doi:10.1017/s000711451300442x

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work is funded by the research grants from the National Natural Science Foundation of China (No. 31500418), the Natural Science Foundation of Jiangsu Province (Grants No. BK20150773), and the Fundamental Research Funds for the Central Universities (No. 30915011101, 30915011310). The authors would like to thank these organizations for financial supports.

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  1. Center for Molecular Metabolism, Nanjing University of Science & Technology, 200 Xiaolingwei Street, Nanjing, 210094, China

    Haiyang Xu, Jing Li, Shiming Wang, Linxiang Xu, Rui Cheng & Jianfa Zhang

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  1. Haiyang Xu
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  2. Jing Li
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  3. Shiming Wang
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  4. Linxiang Xu
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Correspondence to Jing Li.

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Xu, H., Li, J., Wang, S. et al. Oligosaccharide elicitor prepared from Salecan triggers the defense responses of Arabidopsis thaliana Col0 against Botrytis cinerea infection. World J Microbiol Biotechnol 33, 165 (2017). https://doi.org/10.1007/s11274-017-2333-3

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