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Oleuropein protects intracerebral hemorrhage-induced disruption of blood-brain barrier through alleviation of oxidative stress

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Abstract

Background

Intracerebral haemorrhage (ICH) as a devastating form of stroke has remained a public health threat due to lack of FDA-approved therapy. Oxidative stress originated from blood cell degradation products plays a crucial role in the ICH pathogenesis. In this study we evaluated oleuropein, a potent natural antioxidant from olive, in a well-established rat ICH model from overall symptoms to detailed molecular mechanism.

Methods

ICH model was established by collagenase injection to the brain of rats, which were randomly divided into groups with vehicle mock treatment, followed by treatment with different doses of oleuropein via daily intraperitoneal injection post-ICH for 3 days. The overall neurological deficit, brain edema level and blood-brain barrier (BBB) integrity were then measured in different treatment groups. To understand the protection mechanism of oleuropein in ICH, BBB structural components ZO-1 and occludin, oxidative stress and MAPK signalling pathways were also examined.

Results

Oleuropein treatment showed overall alleviation of ICH-associated neurological deficit and brain edema in a dose dependent manner. Consistently, it could preserve the BBB structure and attenuate oxidative stress as well as ICH-induced MAPK activation in brain tissue.

Conclusion

Our study suggests oleuropein could be used as a promising therapeutic agent for ICH.

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Authors and Affiliations

  1. The Second Affiliated Hospital of Suzhou University, Suzhou, China

    Jing Shi & Guofeng Wu

  2. The Affiliated Hospital of Guizhou Medical University, Guiyang, China

    Jing Shi, Guofeng Wu, Xiaohua Zou & Ke Jiang

Authors
  1. Jing Shi
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  2. Guofeng Wu
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  3. Xiaohua Zou
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  4. Ke Jiang
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Correspondence to Guofeng Wu.

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Shi, J., Wu, G., Zou, X. et al. Oleuropein protects intracerebral hemorrhage-induced disruption of blood-brain barrier through alleviation of oxidative stress. Pharmacol. Rep 69, 1206–1212 (2017). https://doi.org/10.1016/j.pharep.2017.05.004

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  • DOI: https://doi.org/10.1016/j.pharep.2017.05.004

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