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Lack of Association Between Endometriosis and N-acetyl transferase 1 (NAT1) and 2 (NAT2) Polymorphisms in a Japanese Population

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Abstract

Objective

We investigated the association between endometriosis and polymorphisms in the N-acetyltransferase 1 (NAT1) and N-acetyltransferase 2 (NAT 2) genes in a Japanese population, having previously demonstrated a positive association with NAT2 polymorphisms in a UK population.

Methods

Genotyping for NAT1 alleles *3, *4, *10, and *11, and NAT2 alleles *4, *5A, *5B, *5C, *6A, and *7B was performed using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) and allele-specific PCR (AS-PCR) analysis in 145 ethnically Japanese, endometriosis patients and 182 controls. The NAT1 and NAT2 allele and genotype frequencies were compared in cases and controls using the Fisher exact test.

Results

No significant differences between cases and controls were observed in the frequencies of the NAT1 and NAT2 alleles (P =. 13; P =.91) and genotypes (P =.24; P =.79), and the NAT2 acetylation phenotypes (P =.46). Dividing the cases into a subgroup, consisting of women with severe disease only (n = 80), had no effect on the results.

Conclusion

The distribution of NAT1 and NAT2 allele and genotype frequencies were not significantly different between Japanese cases and controls. Our findings suggest that polymorphisms in NAT1 and NAT2 are unlikely to be associated with an increased risk of endometriosis in the Japanese population.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-Cho Chuo-ku, Kobe, Japan

    Masashi Deguchi MD, Shigeki Yoshida MD, PhD, Stephen Kennedy MRCOG, Noriyuki Ohara MD, PhD, Satoru Motoyama MD, PhD & Takeshi Maruo MD, PhD

  2. Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Oxford, UK

    Masashi Deguchi MD, Shigeki Yoshida MD, PhD, Stephen Kennedy MRCOG, Noriyuki Ohara MD, PhD, Satoru Motoyama MD, PhD & Takeshi Maruo MD, PhD

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  1. Masashi Deguchi MD
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  2. Shigeki Yoshida MD, PhD
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  3. Stephen Kennedy MRCOG
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  4. Noriyuki Ohara MD, PhD
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  5. Satoru Motoyama MD, PhD
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  6. Takeshi Maruo MD, PhD
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Correspondence to Takeshi Maruo MD, PhD.

Additional information

Supported by a Grant-in-Aid for Scientific Research 16024212 from the Japanese Ministry of Education, Science and Culture and the Japan Association of Obstetricians and Gynecologists Ogyaa-Donation Foundation (JODF).

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Deguchi, M., Yoshida, S., Kennedy, S. et al. Lack of Association Between Endometriosis and N-acetyl transferase 1 (NAT1) and 2 (NAT2) Polymorphisms in a Japanese Population. Reprod. Sci. 12, 208–213 (2005). https://doi.org/10.1016/j.jsgi.2005.01.008

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