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Glutathione S-transferase M1 and T1 gene polymorphisms in Brazilian women with endometriosis

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The glutathione family (GST) genes appear to play a role in the genesis of endometriosis. This case–control study aimed to compare the frequencies of GSTM1 and GSTT1 polymorphisms in women with endometriosis and women without endometriosis.

Methods

Polymerase chain reaction was performed to analyze the GSTM1 and GSTT1 genotypes among women with surgically and histologically confirmed endometriosis (case group n = 121) and in women without evidence of endometriosis confirmed by laparoscopy for investigation the infertility or for laparoscopic tubal sterilization (control group n = 97).

Result(s)

No differences in the frequencies of GSTM1 polymorphism (null genotype) were observed between the cases and controls: odds ratio (OR) = 1.13; 95 % CI 0.656–1.93 (p = 0.659). The GSTT1 polymorphism (null genotype) was more prevalent in the endometriosis group than in the control group (OR = 0.53; 95 % CI 0.94–0.29 (p = 0.039). No relationship between menstrual cycle interval and GSTM1 null genotype frequency was observed in either cases or controls (p = 0.370 and p = 0.664, respectively). In addition, no relationship between menstrual cycle interval and GSTT1 null genotype was observed in cases (p = 0.797) or controls (p = 0.052).

Conclusions

GSTM1 null genotype frequency was similar between cases and controls. The GSTT1 null genotype was more frequent in the control group.

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Abbreviations

CEP-HUJM:

Research Ethics Committee—Julio Muller Hospital University

DNA:

Deoxyribonucleic acid

GSH:

Glutathione

GSTM1:

Gene mu (μ) 1 the glutathione S-transferase system

GSTT1:

Gene theta (θ) 1 the glutathione S-transferase system

BMI:

Body mass index

bp:

Base pairs

PCR:

Polymerase chain reaction

UFMT:

Federal University of Mato Grosso

USA:

United States of America

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Acknowledgments

This study was supported by the National Council of Research and Development (CNPq) and the Mato Grosso Foundation for Research (FAPEMAT).

Conflict of interest

There is no conflict of interest in connection with this article for any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Author information

Authors and Affiliations

  1. Federal University of Mato Grosso—UFMT, Cuiabá, MT, Brazil

    Eloísa Helena Kubiszeski, Sebastião Freitas de Medeiros, Joziane Agnória da Silva Seidel, Jacklyne Silva Barbosa, Marcial Francis Galera & Bianca Borsatto Galera

  2. Department of Gynecology and Obstetrics, Medical School, Federal University of Mato Grosso—UFMT, Cuiabá, MT, Brazil

    Sebastião Freitas de Medeiros

  3. Tropical Institute of Reproductive Medicine and Menopause, Cuiabá, MT, Brazil

    Sebastião Freitas de Medeiros

  4. Department of Basic Sciences, Medical School, Federal University of Mato Grosso—UFMT, Cuiabá, MT, Brazil

    Marcial Francis Galera & Bianca Borsatto Galera

  5. Av. Fernando Corrêa da Costa, n° 2367—Bairro Boa Esperança, Cuiabá, MT, ZIPMAIL: 78060-900, Brazil

    Eloísa Helena Kubiszeski

Authors
  1. Eloísa Helena Kubiszeski
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  2. Sebastião Freitas de Medeiros
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  3. Joziane Agnória da Silva Seidel
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Corresponding author

Correspondence to Eloísa Helena Kubiszeski.

Additional information

Capsule

GSTT1 null polymorphism may protect against endometriosis development in mixed-race Brazilian women.

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Kubiszeski, E.H., de Medeiros, S.F., da Silva Seidel, J.A. et al. Glutathione S-transferase M1 and T1 gene polymorphisms in Brazilian women with endometriosis. J Assist Reprod Genet 32, 1531–1535 (2015). https://doi.org/10.1007/s10815-015-0554-7

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  • DOI: https://doi.org/10.1007/s10815-015-0554-7

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