Abstract
Various extracts obtained from the red alga Plocamium brasiliense (Greville Howe & Taylor), including a fraction containing crude 5-chloro-1-(E)-chlorovinyl-2,4-dibromo-1,5-dimethylcyclohexane (1) and another containing a mixture of halogenated monoterpenes (F), as well as atomaric acid meroditerpene (2) isolated from brown alga Stypopodium zonale (J. V. Lamouroux) Papenfuss, were evaluated for their activity against Trypanosoma cruzi. The cytotoxic and trypanosomicidal effects of these extracts were evaluated in Vero cells and clinically relevant forms of T. cruzi (amastigotes and trypomastigotes). All extracts from P. brasiliense presented low cytotoxicity and moderate trypanosomicidal effects, except for the hydroalcoholic extract. The crude 1 and F fractions had enhanced trypanocidal activity but showed low selectivity. Moreover, atomaric acid (2) was identified as a hit, demonstrating a potent trypanocidal effect reaching an IC50 <10 μM against two different DTU (Yand high selectivity index (<10). These results identify marine natural products as promising candidates against Chagas disease.
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JCRL and CJBR (PhD students) contributed to chromatographic analysis. GCL (PhD student) contributed to biological assay. MCSP, CMC, SCB NO, and VLT designed the study, supervised the laboratory work, and contributed to the critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.
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Teixeira, V.L., Rodrigues Lima, J.C., Lechuga, G.C. et al. Natural products from marine red and brown algae against Trypanosoma cruzi. Rev. Bras. Farmacogn. 29, 735–738 (2019). https://doi.org/10.1016/j.bjp.2019.08.003
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DOI: https://doi.org/10.1016/j.bjp.2019.08.003