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Anti-herpesvirus bovine type 5 activities of extracts obtained from Plocamium brasiliense

  • IV Latin American Congress of Algae Biotechnology (CLABA) and IV Redealgas Workshop
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Abstract

Bovine herpesvirus type 5 (BoHV-5) is an important etiologic agent of meningoencephalitis in cattle and has been frequently identified in outbreaks of neurological disease in bovine in the southern hemisphere including Brazil. This study aimed to evaluate the cytotoxic effect and the antiviral properties of extracts obtained from Plocamium brasiliense (Greville) Howe and Taylor in BoHV-5 RJ42/01 replication. The cytotoxic effects were measured in Madin-Darbin bovine kidney cells (MDBK) and cytotoxic concentration (CC50) values have been determined for acyclovir (ACV) (223 μg ± 2.0), ethyl acetate extract from P. brasiliense (2,109 μg ± 10), hexane extract from P. brasiliense (7.181 μg ± 5), dichloromethane extract from P. brasiliense (2.356 μg ± 6.5), and hydroalcoholic extract from P. brasiliense (1.408 μg ± 5.8). As a first approach to characterize the action of these extracts on BoHV-5 RJ42/01, a virucidal assay activity was performed. A virus suspension containing 1 × 105 plaque-forming units (PFU) of the BoHV-5 RJ42/01 was mixed with 600 μg of extract and acyclovir and kept at room temperature (24 °C) for 3 h. Meanwhile, a control of untreated infected virus was performed in the same conditions. Then, treated virus suspension and untreated control were diluted, and percentage of inhibition of infectivity was determined by plaque assay: ethyl acetate extract (99 %), hexane extract (90 %), dichloromethane extract (99 %), and hydroalcoholic extract (27 %). Acyclovir had a slight virucidal activity on viral particle. The inhibition of attachment was performed in MDBK cells inoculated with 100 PFU of BoHV-5 RJ42/01 in the presence or absence of various concentrations of extracts (0.3, 0.9, and 1.5 μg mL−1). Acyclovir was also assayed at 2.8 and 11.25 μg mL−1. The inhibition of adsorption was also tested in MDBK cells treated with the same concentrations of the extracts before virus inoculation. Results: hexane extracts inhibited virus attachment in pre-treated cell 0.9 μg (55 %) and 1.5 μg (71 %) and untreated MDBK cell only with 1.5 μg (63 %). Ethyl acetate extract on cell pre-treated with 0.3 μg (67 %), 0.9 μg (81 %), and 1.5 μg (91 %). Ethyl acetate extract on pre-treated cell 0.3 μg (67 %), 0.9 μg (81 %), and 1.5 (91 %) but discrete inhibition on cell untreated. Dichloromethane extract and acyclovir slightly inhibited virus binding on MDBK cell.

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Acknowledgments

The authors thank the Brazilian agencies CNPq, CAPES, and FAPERJ and FOPESQ-UFF and FIOCRUZ for the financial support. Pronex-FAPERJ (grant number E-26/110.574/2010) is acknowledged. MDV and ICNPP are recipients of CNPq research fellowships; GBS received a CAPES research fellowship.

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Authors and Affiliations

  1. Universidade Federal Fluminense, Rua Hernani Melo, 101, São Domingos, 24210-130, Niterói, RJ, Brazil

    Ana Maria V. Pinto

  2. Laboratório de Virologia Comparada e Ambiental, Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, 21040-360, Rio de Janeiro, RJ, Brazil

    Ana Maria V. Pinto & José Paulo G. Leite

  3. Instituto de Biologia, Universidade Federal Fluminense, Campus do Valonguinho, Centro, 24020-141, Niterói, RJ, Brazil

    Ana Maria V. Pinto & Carlos José Brito Ramos

  4. Programa de Pós-Graduação em Ciências e Biotecnologia (PPBI) Instituto de Biologia (EGB), Universidade Federal Fluminense, Campus do Valonguinho, Centro, 24020-141, Niterói, RJ, Brazil

    Ana Maria V. Pinto, Carlos José Brito Ramos, Valéria Laneuville Teixeira & Izabel Christina N. P. Paixão

  5. Programa de Pós-Graduação em Química, Universidade Federal Fluminense, Outeiro, 24020-141, Niterói, RJ, Brazil

    Rainiomar Raimundo da Fonseca

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  1. Ana Maria V. Pinto
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  2. José Paulo G. Leite
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Correspondence to Ana Maria V. Pinto or Izabel Christina N. P. Paixão.

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Pinto, A.M.V., Leite, J.P.G., Ramos, C.J.B. et al. Anti-herpesvirus bovine type 5 activities of extracts obtained from Plocamium brasiliense . J Appl Phycol 26, 2021–2027 (2014). https://doi.org/10.1007/s10811-014-0319-1

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  • DOI: https://doi.org/10.1007/s10811-014-0319-1

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