Abstract
This study aimed to prepare hydrogel containing Cymbopogon citratus (DC.) Stapf, Poaceae, volatile oil encapsulated in poly (d, l-lactide-co-glycolide) nanoparticles and to evaluate its in vitro anti-herpetic activity. Polymeric nanoparticles were prepared by solvent emulsification-diffusion method and incorporated in carbomer hydrogels. In vitro release profiles for the nanogel, loaded nanoparticles and hydrogel containing free oil were evaluated by dialysis. Inhibitory activities against Herpes simplex for the formulations were investigated in Vero cells. Hydrogel was developed using nanoparticles with mean diameter of 217.1 nm and negative Zeta potential (−20.5mV). Volatile oil release profile showed a biphasic pattern with an initial faster release and subsequent sustained phase in all formulations. Nanogel strongly inhibited virus in a non-cytotoxic concentration, 42.16 times lower than free oil, 8.76 and 2.23 times than loaded nanoparticles and hydrogel containing free oil, respectively. These results highlight the potential of nanogel to protect oil against volatilization, control release and improve its anti-herpetic activity.
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KBA contributed running the laboratory work, analysis of the data and drafted the paper. ACFA contributed in collecting plant sample and identification, and confection of herbarium. JLA contributed to development and characterization of the formulations. SCM contributed to in vitro CcVO release studies. JFC and MTVR contributed to antiviral activity studies. DQF designed the study, supervised the laboratory work and contributed to critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.
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Almeida, K.B., Araujo, J.L., Cavalcanti, J.F. et al. In vitro release and anti-herpetic activity of Cymbopogon citratus volatile oil-loaded nanogel. Rev. Bras. Farmacogn. 28, 495–502 (2018). https://doi.org/10.1016/j.bjp.2018.05.007
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DOI: https://doi.org/10.1016/j.bjp.2018.05.007