Abstract
Monitoring phycotoxin accumulation in marine products such as edible shellfish is a regulatory requirement in many countries. Therefore, a simple and rapid onsite quantification method is sought. Herein, we present a fluorescence polarization immunoassay (FPIA), a well-known one-step immunoassay, using a portable fluorescence polarization analyzer for domoic acid (DA), widely referred to as the primary toxin of amnesic shellfish poisoning (ASP). To establish FPIA for DA, the matrix effect of methanol, which is widely used to extract DA from shellfish, on FPIA was investigated. To validate this method, we performed a spike recovery test using oysters containing DA at a concentration equivalent to the regulatory limits of North America and the European Union (20 mg/kg). The recovery rate was found to be 79.4–114.7%, which is equivalent to that of the commercially available enzyme-linked immunosorbent assay (ELISA). We expect that this FPIA system will enable the quantitative onsite analysis of DA and significantly contribute to the safety of marine products.
Graphical Abstract
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Many of the marine toxins are phycotoxins produced by phytoplankton, and they accumulate in shellfish tissues through biomagnification in the food chain [1]. Shellfish poisoning caused by marine toxins includes paralytic shellfish poisoning, diarrheal shellfish poisoning, and amnesic shellfish poisoning (ASP). These are serious threats to public health and fisheries in temperate to cold waters [1,2,3]. Domoic acid (DA; Fig. 1), widely referred to as the primary toxin of ASP, is produced by marine algae. DA is produced by the diatom the genus Pseudo-nitzschia, a type of phytoplankton that accumulates in bivalve shellfish [1,2,3]. The regulatory limit for DA in seafood in North America and the European Union is 20 mg/kg [1, 4].
For the detection of marine toxins contained in seafoods, historically, an animal-based assay was used. However, it was replaced by chemical assays because the animal-based method not only has ethical problems but also is expensive and has low reproducibility [1, 4] For DA detection, high-performance liquid chromatography (HPLC) was widely adopted as an official method [1, 5, 6]. However, this method has drawbacks such as the requirement of skilled personnel and expensive and large-scale systems. A mass spectrometry method was developed from the perspective of improving analytical throughput [7]; however, this technique also requires expensive systems and skilled personnel.
For improving cost-effectiveness in terms of the time, delivery, and human resources, a simple and rapid onsite quantification method for DA is desired. Accordingly, various immunoassays have been developed [8, 9]. For example, an enzyme-linked immunosorbent assay (ELISA) was developed for DA quantification [8, 10, 11] and ELISA kits are currently commercially available [12]. Although ELISA has high sensitivity and enables quantification, it requires multiple washing cycles, which renders the operation complicated and time-consuming. Recently, a lateral-flow immunoassay was reported for DA detection [9, 13]. Although this method has a high potential on the rapid detection of DA, quantitative portable analysis has not been demonstrated. Herein, we present a fluorescence polarization immunoassay (FPIA) that enables rapid quantification of DA with a portable analyzer. The FPIA is a well-known single-step immunoassay in which the analyte is quantified by measuring the degree of fluorescence polarization (P) [14, 15]. This method is based on the competitive binding of the analyte (antigen) and a fluorescence-labeled analyte (tracer) to the antibody (Fig. 2a). The ratio of the free tracer to bound tracer reflects the analyte concentration, which can be determined from the degree of P. When polarized light is used for excitation, the smaller free molecule emits depolarized fluorescence owing to its fast rotational diffusion, resulting in low P, whereas the larger bound tracer exhibits the opposite tendency (Fig. 2b). Recently, we developed a portable FPIA analyzer for the onsite analysis of analytes (Fig. 3) [16, 17]. The potential of this analyzer for onsite analysis has been demonstrated by the rapid measurement protein biomarkers [18, 19] and viruses in serum [20]. We have reported that this analyzer can be used for the detection of mycotoxins in wheat-based foods which are pretreated by using the conventional extraction method [21]. However, the matrix effects of animal tissues such as shellfish have not been investigated yet. In this study, we established a procedure for DA quantification using the FPIA and demonstrate a spike-recovery test for DA present in oysters using the portable analyzer.
Materials and methods
Reagents
Methanol (CH3OH) and phosphate-buffered saline (PBS) were purchased from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan). Domoic Acid (DA) was obtained from Toronto Research Chemicals Inc. (Toronto, Canada). Fluorescein isothiocyanate-labeled DA (FDA) was synthesized by SCRUM Inc. (Tokyo, Japan), according to a previously reported procedure [20]. The EuroProxima Domoic acid ELISA kit was obtained from R-Biopharm Nederland B.V. (Arnhem, The Netherlands).
Measurement of the degree of fluorescence polarization (P) using the portable FPIA analyzer
We measured P using a portable FPIA analyzer that was previously developed in our groups (Fig. 3a) and disposable microfluidic devices with nine microchannels (Fig. 3b and c) [16, 17]. The microfluidic device is made of black poly (dimethylsiloxane) and a glass plate. Typically, 20–30 µL of sample solutions were injected into the nine channels of the microfluidic device (width 200 µm, height 900 µm) by a 100 µL-sized micropipette, and the fluorescence from the samples in the microchannels was detected simultaneously using a CCD camera (Fig. 3c) to evaluate the P values of nine samples independently.
Preparation of FDA dilution series and verification of optimal concentration
Aqueous solutions of FDA in PBS were prepared at 0.143–14,300 nM concentrations. Each solution was injected into the microchannels in the device, and the P of each solution was measured using the portable analyzer.
Verification of the effect of methanol on P measurement
Samples containing FDA (1.43 nM), DA (0 nM or 64 nM), 0.00–0.13% (v/v) methanol, and anti-DA antibodies were prepared in PBS. As an anti-DA antibody, we used the antibody in the DA ELISA kit. After incubation at room temperature for 1 h, the samples were injected into the microfluidic device and P was measured using a portable analyzer.
Addition and extraction of DA in shellfish for spike-recovery tests
Raw edible oysters (Crassostrea gigas) were used as an example of shellfish. The soft body of the oyster was ground using an electric hand blender and processed into a paste. Next, 100 µL of a 400 µg/mL DA solution was added to 2.0 g of the shellfish paste, and the resulting mixture was placed in a 15 mL test tube and mixed by tapping. After 30 min of incubation at room temperature, DA was extracted from the oyster paste using the methanol extraction method [4, 22] adopted in the approved official method [6]. For this, 2 mL of Milli-Q water was added to the oyster paste and the mixture was vortexed for 1 min. Then, 4 mL of methanol was added, and the mixture was vortexed for 3 min. Finally, the mixture was centrifuged at 2000 × g for 10 min, and the supernatant was collected and filtered through a Millipore® Millex® syringe filter (0.45 µm pore size; Merck KGaA, Darmstadt, Germany). The filtration using the syringe filter takes 3 min. A portion of the supernatant of the extract was dialyzed using Millipore® Amicon® Ultra (nominal molecular weight cutoff: 10 kDa, Merck KGaA, Darmstadt, Germany) to eliminate macromolecular contaminants (30 min).
Spike-recovery test using the FPIA
A calibration curve of DA was constructed using standard solutions containing 0.26 nM to 64 nM of DA, 1.43 nM FDA, anti-DA antibody, and 0.01% (v/v) methanol in PBS. As an analyte solution, a mixture of the oyster extract diluted by 5,000-fold, 1.43 nM FDA, anti-DA antibody, and 0.01% (v/v) methanol was prepared in PBS. After 10, 30, and 60 min of incubation, the solutions were injected into the microfluidic device and P was measured using the portable analyzer. The limit of detection (LOD) was calculated as the DA concentration corresponding to the PLOD = Pave + 3 s, where Pave and s are the mean and standard deviation of P with the lowest DA concentration (0.143 nM), respectively [19].
Spike-recovery test using ELISA
DA in the oyster extract was also determined using the ELISA kit, according to the manufacturer’s instructions.
Results and discussion
First, the P values of FDA aqueous solutions with different FDA concentrations were evaluated by the FPIA using a portable analyzer. In this experiment, P should be constant regardless of the FDA concentration, because the solution contains only free FDA molecules. However, as shown in Fig. 4, the value of P was constant above 1.43 nM, but increased below 1.43 nM. In the low-concentration condition, certain interfering factors, such as the fluorescence of PDMS and the adsorption of FDA to the microchannel walls, may affect the P value. Since the lower tracer concentration is better from the viewpoints of the sensitivity of FPIA and the consumption of the antibody, we chose to use 1.43 nM FDA in subsequent experiments. The dilution factor of the antibody was optimized by using 1.43 nM FDA.
Next, we confirmed the effect of methanol addition on the FPIA, because methanol is commonly used to extract DA from shellfish in the official methods of DA quantification [4, 22]. As shown in Fig. 5, although the P value of tracer-antibody complex (DA 0 nM) is higher than that of free tracer (DA 64 nM), which indicates the antibody can bind to the tracer with the existence of methanol, the addition of methanol increased the P value. One possible reason of P increase is that the viscosity of the methanol–water mixture in low methanol concentration range is slightly higher than that of pure water [23] even though the viscosity of pure methanol is lower than that of pure water. For DA detection, methanol was added to the standard solutions used for constructing the calibration curve to compensate for the matrix effect.
Based on the above investigations, a spike-recovery test of DA in oysters was performed. Nine solutions, including seven DA standard solutions and two oyster extract samples, were analyzed at the same time using the microfluidic device with nine microchannels. A calibration curve was obtained for P using the standard solutions of DA (Fig. 6). The limit of detection (LOD) was calculated as 0.97 nM by fitting the sigmoidal function by following the previous study [19]. Oyster-based samples prepared with and without dialysis were evaluated because FPIA is sometimes affected by contaminant proteins in the sample solution. The DA concentration of the oyster extract was determined before and after dialysis using the calibration curve. The DA concentrations in the respective samples were 3.67 nM (22.8 mg/kg of oyster, recovery, 114.7%) and 2.54 nM (15.8 mg/kg of oyster, recovery, 79.4%), respectively (Table 1). These results reveal that FPIA can quantify DA, regardless of the dialysis of the oyster extract. Investigation of the reaction time indicated that a reaction time longer than 20 min was sufficient for DA quantification (Figure S1). Since the accuracy of FPIA is not significantly different with and without dialysis, dialysis can be omitted to reduce analysis time. A comparison of the results of FPIA and ELISA indicated that the recovery rates of the two assays are comparable (Table 1). In addition, the comparison with other DA quantification methods revealed that this method achieves both high portability and short measurement time (Table S1). This result indicates the potential applicability of FPIA as an onsite method for the quantification of DA in the future. We expect that this FPIA portable analyzer will be applied to other phycotoxin detection using FPIA [24, 25].
Conclusion
In this study, we developed a DA quantification method using a portable FPIA analyzer. The investigation of the matrix effect of the methanol–water mixture showed that P increased with the addition of methanol and matrix matching was required for FPIA. A Spike recovery test of DA in oysters indicated that the developed FPIA could quantify DA with recovery similar to that of commercial ELISA. This method is expected to be useful in the onsite quantification of DA because of the simple operation and portability of the equipment. We expect that the cost of this immunoassay method will be similar to an ELISA kit ($10 ~ 100 /test) in the future. Compared to LFIA, which is a typical portable on-site testing, this method is expected to be more quantitative and reproducible. In addition, since FPIA is a one-step homogeneous immunoassay, it requires less expertise than other multistep immunoassays such as ELISA and other instrumental analyses such as HPLC and LC-mass spectrometry. Therefore, we consider that this method will be a powerful tool for on-site analysis of DA and will enable us to reduce various costs such as labor and transportation.
Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
J.L.C. Wright, Dealing with seafood toxins: present approaches and future options. Food Res. Int. 28, 347–358 (1995). https://doi.org/10.1016/0963-9969(95)00001-3
L. Mos, Domoic acid: a fascinating marine toxin. Environ. Toxicol. Pharmacol. 9, 79–85 (2001). https://doi.org/10.1016/s1382-6689(00)00065-x
J.L.C. Wright, R.K. Boyd, A.S.W. de Freitas, M. Falk, R.A. Foxall, W.D. Jamieson, M.V. Laycock, A.W. McCulloch, A.G. McInnes, P. Odense, V.P. Pathak, M.A. Quillam, M.A. Ragan, P.G. Sim, P. Thibault, J.A. Walter, M. Gilgan, D.J.A. Richard, D. Dewar, Identification of domoic acid, a neuroexcitatory amino acid, in toxic mussels from eastern Prince Edward Island. Can. J. Chem. 67, 481–490 (1989). https://doi.org/10.1139/v89-075
G. Hallegraeff, D.M. Anderson, A. Cembella, Manual on harmful marine microalgae, in UNESCO Publishing. ed. by A. Cembella (Cham, 1995), pp.247–265
M.A. Quilliam, P.G. Sim, A.W. Mcculloch, A.G. Mcinnes, High-performance liquid chromatography of domoic acid, a marine neurotoxin, with application to shellfish and plankton. Int. J. Environ. Anal. Chem. 36, 139–154 (1989). https://doi.org/10.1080/03067318908026867
AOAC Official Method 991.26. Domoic acid in mussels. Liquid chromatographic. In: Official Methods of Analysis of AOAC International. 2000 (cited 2022 Nov 100). http://www.eoma.aoac.org/methods/info.asp?ID=28606. Accessed 10 Nov 2022
D.G. Beach, C.M. Walsh, P. Cantrell, W. Rourke, S. O’Brien, K. Reeves, P. McCarron, Laser ablation electrospray ionization high-resolution mass spectrometry for regulatory screening of domoic acid in shellfish. Rapid Commun. Mass Spectrom. 30, 2379–2387 (2016). https://doi.org/10.1002/rcm.7725
A.F.U.H. Saeed, S. Ling, J. Yuan, S. Wang, The preparation and identification of a monoclonal antibody against domoic acid and establishment of detection by indirect competitive ELISA. Toxins 9, 250 (2017). https://doi.org/10.3390/toxins9080250
W. Jawaid, J. Meneely, K. Campbell, M. Hooper, K. Melville, S. Holmes, J. Rice, C. Elliott, Development and validation of the first high performance-lateral flow immunoassay (HP-LFIA) for the rapid screening of domoic acid from shellfish extracts. Talanta 116, 663–669 (2013). https://doi.org/10.1016/j.talanta.2013.07.027
M. Dubois, L. Demoulin, C. Charlier, G. Singh, S.B. Godefroy, K. Campbell, Development of ELISAs for detecting domoic acid, okadaic acid, and saxitoxin and their applicability for the detection of marine toxins in samples collected in Belgium. Food Addit. Contam. Part A 27, 859–868 (2010). https://doi.org/10.1080/19440041003662881
R.W. Litaker, T.N. Stewart, B.-T.L. Eberhart, J.C. Wekell, V.L. Trainer, R.M. Kudela, P.E. Miller, A. Roberts, C. Hertz, T.F. Johnson, G. Frankfurter, G.J. Smith, A. Schnetzer, J. Schumacker, J.L. Bastian, A. Odell, P. Gentien, D. Le Gal, D.R. Hardison, P.A. Tester, Rapid enzyme-linked immunosorbent assay for detection of the algal toxin domoic acid. J. Shellfish Res. 27, 1301–1310 (2008). https://doi.org/10.2983/0730-8000-27.5.1301
K. Petropoulos, S.F. Bodini, L. Fabiani, L. Micheli, A. Porchetta, S. Piermarini, G. Volpe, F.M. Pasquazzi, L. Sanfilippo, P. Moscetta, S. Chiavarini, G. Palleschi, Re-modeling ELISA kits embedded in an automated system suitable for on-line detection of algal toxins in seawater. Sens Actuators B Chem 283, 865–872 (2019). https://doi.org/10.1016/j.snb.2018.12.083
O.D. Hendrickson et al., Rapid detection of phycotoxin domoic acid in seawater and seafood based on the developed lateral flow immunoassay. Anal. Methods 14, 2446–2452 (2022). https://doi.org/10.1039/D2AY00751G
O.D. Hendrickson, N.A. Taranova, A.V. Zherdev, B.B. Dzantiev, S.A. Eremin, Fluorescence polarization-based bioassays: new horizons. Sensors 20, 7132 (2020). https://doi.org/10.3390/s20247132
H. Zhang, S. Yang, K. De Ruyck, N.V. Beloglazova, S.A. Eremin, S. De Saeger, S. Zhang, J. Shen, Z. Wang, Fluorescence polarization assays for chemical contaminants in food and environmental analyses. Trends Anal. Chem. 114, 293–313 (2019). https://doi.org/10.1016/j.trac.2019.03.013
O. Wakao, K. Satou, A. Nakamura, K. Sumiyoshi, M. Shirokawa, C. Mizokuchi, K. Shiota, M. Maeki, A. Ishida, H. Tani, K. Shigemura, A. Hibara, M. Tokeshi, A compact fluorescence polarization analyzer with high-transmittance liquid crystal layer. Rev. Sci. Instrum. 89, 024103 (2018). https://doi.org/10.1063/1.5017081
O. Wakao, Y. Fujii, M. Maeki, A. Ishida, H. Tani, A. Hibara, M. Tokeshi, Fluorescence polarization measurement system using a liquid crystal layer and an image sensor. Anal. Chem. 18, 9647–9652 (2015). https://doi.org/10.1021/acs.analchem.5b01164
K. Nishiyama, K. Takahashi, M. Fukuyama, M. Kasuya, A. Imai, T. Usukura, N. Maishi, M. Maeki, A. Ishida, H. Tani, K. Hida, K. Shigemura, A. Hibara, M. Tokeshi, Facile and rapid detection of SARS-CoV-2 antibody based on a noncompetitive fluorescence polarization immunoassay in human serum samples. Biosens. Bioelectron. 190, 113414 (2021). https://doi.org/10.1016/j.bios.2021.113414
M. Fukuyama, A. Nakamura, K. Nishiyama, A. Imai, M. Tokeshi, K. Shigemura, A. Hibara, Noncompetitive fluorescence polarization immunoassay for protein determination. Anal. Chem. 92, 14393–14397 (2020). https://doi.org/10.1021/acs.analchem.0c02300
K. Nishiyama, Y. Takeda, K. Takahashi, M. Fukuyama, M. Maeki, A. Ishida, H. Tani, K. Shigemura, A. Hibara, H. Ogawa, M. Tokeshi, Non-competitive fluorescence polarization immunoassay for detection of H5 avian influenza virus using a portable analyzer. Anal. Bioanal. Chem. 413, 4619–4623 (2021). https://doi.org/10.1007/s00216-021-03193-y
A. Nakamura, M. Aoyagi, M. Fukuyama, M. Maeki, A. Ishida, H. Tani, K. Shigemura, A. Hibara, M. Tokeshi, Determination of deoxynivalenol in wheat, barley, corn meal, and wheat-based products by simultaneous multisample fluorescence polarization immunoassay using a portable analyzer. ACS Food Sci. Technol. 1, 1623–1628 (2021). https://doi.org/10.1021/acsfoodscitech.1c00244
M.A. Quilliam, M. Xie, W.R. Hardstaff, Rapid extraction and cleanup for liquid chromatographic determination of domoic acid in unsalted seafood. J. AOAC Int. 78, 543–554 (1995). https://doi.org/10.1093/jaoac/78.2.543
S.Z. Mikhail, W.R. Kimel, Densities and viscosities of methanol-water mixtures. J Chem Eng Data 6, 533–537 (1961). https://doi.org/10.1021/je60011a015
H. Zhang, S. Yang, R.C. Beier, N.V. Beloglazova, H. Lei, X. Sun, Y. Ke, S. Zhang, Z. Wang, Simple, high efficiency detection of microcystins and nodularin-R in water by fluorescence polarization immunoassay. Anal Chim Acta 992, 119–127 (2017). https://doi.org/10.1016/J.ACA.2017.09.010
H. Zhang, B. Li, Y. Liu, H. Chuan, Y. Liu, P. Xie, Immunoassay technology: Research progress in microcystin-LR detection in water samples. J Hazard Mater. 424, 127406 (2022). https://doi.org/10.1016/J.JHAZMAT.2021.127406
Acknowledgements
This work was supported by the Strategic International Collaborative Research Project promoted by the Ministry of Agriculture, Forestry and Fisheries, Japan (JPJ008837) and the Russian Science Foundation (Project 20-43-07001). We would like to acknowledge Dr. Toshiyuki Suzuki of the Fisheries Technology Institute, Japan Fisheries Research and Education Agency for helpful discussions. M.F. acknowledges Ms. Keiko Sakai for technical support.
Author information
Authors and Affiliations
Contributions
Conceptualization: MT, AH, Methodology: MF, MT, AH, Investigation: YO, MF, MK, Funding acquisition: MT, AH. Software; KS, Supervision: MF, AH, Writing—original draft: YO, MF, Writing—review & editing: YO, MF, MK, KS, SAE, MT, AH.
Corresponding authors
Ethics declarations
Conflict of interest
There are no conflicts to declare.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Ogura, Y., Fukuyama, M., Kasuya, M. et al. Rapid determination of domoic acid in seafood by fluorescence polarization immunoassay using a portable analyzer. ANAL. SCI. 39, 2001–2006 (2023). https://doi.org/10.1007/s44211-023-00413-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s44211-023-00413-6