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Developing genetic stock for yellow and brown rust resistance in Triticum aestivum L. via Imperata cylindrica-mediated doubled haploidy technique

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Abstract

In vitro haploid production followed by chromosome doubling greatly enhances the production of complete homozygous wheat lines in a single generation. This work aimed to develop wheat doubled haploid genetic stock resistant to yellow and brown rust via intergeneric hybridization with Imperata cylindrica and its further characterization. The wheat doubled haploid (DH) line was developed by crossing wheat F1 (HS 542/China 84-40,022) with I. cylindrica. This DH-1 was tested against eight races of yellow (110S119, 110S247, 238S119, 78S84, 110S84, 111S68, T and P) and eighteen races of brown rust (11,12-5, 12-7, 12A, 77, 77-1, 77-2, 77-5, 77-7, 77-8, 77-10, 77A-1, 104-2, 107-1, 108-1, 162-1, 77-9 and 104-1) at seedling stage and with mix race of yellow and brown rust in adult plant stage (at Dhaulakuan, Bajaura and Shimla). At the seedling stage, DH-1 showed resistance to all the yellow and brown rust pathotypes except for 77-5 race of brown rust. The adult plant response of this DH line also showed resistance to both yellow and brown rust. Based on host pathogen interaction, the DH-1 was postulated to carry leaf rust resistant genes Lr26 + Lr23 + 1 + and yellow rust resistant gene Yr9 + gene. Molecular characterization of the DH line with gene-specific primers showed that the developed DH-1 contains leaf rust resistance genes Lr34, Lr26 and Lr32. The DH-1 has semi-spreading growth habit, erect flag leaf attitude, plant height of 94 cm, have scurs and 1000 grain weight of 36 g. The results highlight I. cylindrica-mediated chromosome elimination technique of doubled haploid production as an effective tool for the development of wheat rust resistant lines in the shortest timeframe. This is the first report of genetic stock development for rust resistance in wheat via I. cylindrica-mediated DH technique.

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Acknowledgements

The authors would like to express their utmost gratitude to SERB for the financial support by funding a project on DH production in wheat. Help received from ICAR-IARI New Delhi, CSK HPKV, Palampur, ICAR-IIWBR, RS, Flowerdale, Shimla and ICAR-CPRI, Shimla, is highly acknowledged.

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Authors and Affiliations

  1. ICAR-Indian Agricultural Research Institute, Regional Station, Shimla, Himachal Pradesh, 171004, India

    Madhu Patial, Neha Sharma, Dharam Pal, K. K. Pramanick & Ruchi Chauhan

  2. Cytogenetics and Tissue Culture Lab, Department of Plant Breeding and Genetics, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh, 176062, India

    H. K. Chaudhary

  3. ICAR-Indian Institute of Wheat and Barley Research, Himachal Pradesh, Flowerdale, Shimla, 171002, India

    O. P. Gangwar & S. C. Bhardwaj

  4. CSKHPKV, HAREC, Bajaura, Kullu, Himachal Pradesh, India

    Naval Kishore

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  1. Madhu Patial
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  2. H. K. Chaudhary
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  5. Naval Kishore
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  8. S. C. Bhardwaj
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  9. Ruchi Chauhan
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Contributions

MP and HKC contributed to conceptualization of research; MP and HKC contributed to designing of experiments; MP and NS contributed to execution of lab experiments; MP, OPG, NK and SCB contributed to execution of field work; MP and NS were involved in analysis and data analysis; and MP, DP, KKP and RC contributed to preparation of manuscript.

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Correspondence to Madhu Patial.

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Communicated by J. Zimny.

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Patial, M., Chaudhary, H.K., Sharma, N. et al. Developing genetic stock for yellow and brown rust resistance in Triticum aestivum L. via Imperata cylindrica-mediated doubled haploidy technique. CEREAL RESEARCH COMMUNICATIONS 50, 439–448 (2022). https://doi.org/10.1007/s42976-021-00180-y

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