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ABA-induced serine/threonine protein kinase gene transcribed in rye (Secale cereale L.)

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Abstract

We isolated the rye serine/threonine protein kinase (S/T PK) gene, which is responsible for intracellular signal transduction mediated by abscisic acid (ABA) synthesized following drought stress, from the huge genome, i.e., 1Cx = 7917 Mb. cDNA was synthesized from poly(A)+ RNA from ABA-treated leaves and then ligated with pUC118 via EcoRI linker to form a cDNA library. RT-PCR amplified DNA fragment of S/T PK gene was used as a probe to screen the cDNA library. One positive cDNA clone was sequenced to determine the overall 1462 bp of the S/T PK gene, which encodes the S/T PK protein consisting of deduced 363 amino acid residues including 12 catalytic subdomains. Total RNA was extracted from both ABA-treated and ABA-untreated rye Petkus leaves and then run on a 1% agarose gel denatured by formaldehyde, and the RNA was transblotted to a nylon membrane. Next, northern hybridization was conducted on the membrane using the 3′ untranslated 244 bp region as probes. The rye S/T PK gene was transcribed in ABA-treated leaves but not in untreated leaves. We have shown that rye S/T PK, localized on the 2R chromosome, is transcribed via ABA mediation, which indicates the relation to intracellular signal transduction.

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Acknowledgements

Thanks are extended to the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT) for the Grant-in-Aid for Scientific Research No. 04760006 that supported this work to M.T.

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Authors and Affiliations

  1. Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, Shizuoka, 422-8529, Japan

    M. Tomita

  2. Faculty of Agriculture, Tottori University, Tottori, Tottori, 680-8553, Japan

    H. Tanaka & K. Takahashi

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  1. M. Tomita
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  2. H. Tanaka
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  3. K. Takahashi
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Correspondence to M. Tomita.

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Tomita, M., Tanaka, H. & Takahashi, K. ABA-induced serine/threonine protein kinase gene transcribed in rye (Secale cereale L.). CEREAL RESEARCH COMMUNICATIONS 49, 21–30 (2021). https://doi.org/10.1007/s42976-020-00064-7

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