Abstract
Cordyceps acid is an active component of Cordyceps cicadae and has a variety of medicinal uses, including anti-tumor effects, the prevention of cerebral hemorrhaging and myocardial infarction, and the inhibition of a wide range of bacteria. The objectives of this study were to identify C. cicadae fungi and optimize the culture conditions to obtain a high yield of cordycepic acid. First, a wild C. cicadae was identified by morphological observation and rDNA sequence analysis. Secondly, the optimal fermentation conditions were determined using a single-factor method, a Plackett–Burman design, and a Box–Behnken response surface. Finally, using the yield of fruit bodies and the content of cordyceps acid as indices, combined with a single-factor experiment and a response surface design, the best combination of conditions for cultivation was determined. The results showed that the best combination was as follows: sucrose 2%, tryptone 2%, KH2PO4 0.4%, MgSO4·7H2O 0.4%, an initial pH of the fermentation liquid of 7.0, 5% inoculum, fermentation for 4.5 d, a ratio of medium to liquid of 1:1.7, illumination intensity 150 Lux, illumination time 15 h per day, and 70% humidity. The content of cordycepic acid in the fruiting bodies developed in cultivation was 2.07-fold higher than that in the wild C. cicadae. This study provides a theoretical basis for the large-scale cultivation of C. cicadae with a high concentration of cordycepic acid.
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Funding
This work was supported by the National Science Foundation of China (Grant No. 31501461), the Key Scientific Research Foundation of Anhui Province Education Department (Grant No. KJ2021A0959), and the Science and Technology Planning Project of Huainan (Grant No. 2021A2410).
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Cuie Shi conceived and designed the study. Wenlong Song and Jian Gao wrote the manuscript. Wenlong Song, Chen Guo, and Tengfei Zhang conducted the experiments. Cuie Shi, Wenlong Song, and Shoubao Yan analyzed the data. Cuie Shi and Shoubao Yan revised the manuscript. All authors contributed to the article and approved the submitted version.
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Cuie Shi, Wenlong Song, Jian Gao, Shoubao Yan, Chen Guo, and Tengfei Zhang declare that they have no conflict of interest.
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Shi, C., Song, W., Gao, J. et al. Enhanced production of cordycepic acid from Cordyceps cicadae isolated from a wild environment. Braz J Microbiol 53, 673–688 (2022). https://doi.org/10.1007/s42770-022-00687-4
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DOI: https://doi.org/10.1007/s42770-022-00687-4