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Fungal endophytes from leaves of Mandevilla catimbauensis (Apocynaceae): diversity and potential for L-asparaginase production

  • Environmental Microbiology - Research Paper
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Abstract

In this study, we examined endophytic fungi in leaves of Mandevilla catimbauensis, an endemic plant species found in the Brazilian dry forest (Caatinga), and endophytic fungi’s potential to produce L-asparaginase (L-ASNase). In total, 66 endophytes were isolated, and the leaf-fragment colonisation rate was 11.78%. Based on morphology, internal transcribed spacer (ITS), and partial large subunit (LSU) of ribosomal DNA sequencing, the endophytic fungi isolated belonged to six Ascomycota orders (Botryosphaeriales, Capnodiales, Diaporthales, Eurotiales, Marthamycetales, and Pleosporales). Phyllosticta species were the most frequent endophytes isolated (23 isolates [45.1%] from two species). The Shannon–Wiener and Fisher alpha index average values were 0.56 and 3.26, respectively. Twenty endophytes were randomly selected for the L-ASNase production test, of which fourteen isolates showed potential to produce the enzyme (0.48–2.22 U g−1), especially Phyllosticta catimbauensis URM 7672 (2.22 U g−1) and Cladosporium sp. G45 (2.11 U g−1). Phyllosticta catimbauensis URM 7672 was selected for the partial optimisation of L-ASNase production because of its ability to generate considerable amounts of enzyme. We obtained the highest L-ASNase activity (3.47 U g−1), representing an increase of 36.02% in enzymatic production, under the following experimental conditions: a pH of 4.2, 1.0% inoculum concentration, and 2.5% L-asparagine concentration. Our study showed that M. catimbauensis harbours an important diversity of endophytic fungi with biotechnological potential for L-ASNase production.

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Data availability

Plant specimen is deposited in the Herbário UFP – Geraldo Mariz and endophytic fungi cultures in the URM culture collection, both collections at the Universidade Federal de Pernambuco (Recife, Brazil). DNA sequences are deposited in the GenBank database and sequence alignment in TreeBASE.

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Acknowledgements

The authors sincerely thank the Programa de Pós-Graduação em Biociência Animal of the Universidade Federal Rural de Pernambuco (UFRPE) and all members of the Departamento de Micologia Prof. Chaves Batista and of the Laboratório de Micologia Ambiental (both at the Universidade Federal de Pernambuco). We especially thank Dr. Laura Paiva, Dr. Alexandre Machado, Dr. Karla Freire, Dr. Renan Barbosa, Ana Patrícia Pádua (MSc), Aline Barboza (MSc), Tamara Caldas (MSc), and Thays Oliveira (MSc).

Funding

Fundação de Amparo à Ciência e Tecnologia de Pernambuco (FACEPE, financial support and scholarship IBPG-0970-5.05/14), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) in Brazil.

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Author notes

  1. Gianne R. Araújo-Magalhães and Marília H.C. Maciel contributed equally to this work.

Authors and Affiliations

  1. Programa de Pós-Graduação em Biociência Animal, Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil

    Gianne R. Araújo-Magalhães

  2. Departamento de Micologia Prof. Chaves Batista, Universidade Federal de Pernambuco, Recife, PE, Brazil

    Marília H. C. Maciel, Leticia F. da Silva & Cristina M. Souza-Motta

  3. Departamento de Estatística e Matemática Aplicada, Universidade Federal do Ceará, Fortaleza, CE, Brazil

    Gualberto S. Agamez-Montalvo

  4. Sierra Nevada Research Institute, University of California, Merced, CA, USA

    Iolanda R. da Silva

  5. Setor de Micologia, Departamento de Biociências e Tecnologia, Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil

    Jadson D. P. Bezerra

  6. Laboratório de Microbiologia, Tecnologia Enzimática e Bioprodutos, Universidade Federal do Agreste de Pernambuco (UFAPE), Garanhuns, PE, Brazil

    Keila A. Moreira

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Contributions

Gianne R. Araújo-Magalhães, Cristina M. Souza-Motta, and Keila A. Moreira implemented the project; Gianne R. Araújo-Magalhães collected the plants; Gianne R. Araújo-Magalhães and Leticia F. da Silva performed the fungal endophytes isolation and L-asparaginase production parts of the study; Gualberto S. Agamez-Montalvo designed the experiment and statistically analysed the L-Asparaginase production results; Iolanda R. da Silva performed the ecological analysis; Jadson D.P. Bezerra performed the phylogenetic analysis; Gianne R. Araújo-Magalhães, Marília H.C. Maciel, and Jadson D.P. Bezerra wrote the paper. All the authors reviewed and approved the final version of the paper.

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Correspondence to Jadson D. P. Bezerra or Keila A. Moreira.

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Araújo-Magalhães, G.R., Maciel, M.H.C., da Silva, L.F. et al. Fungal endophytes from leaves of Mandevilla catimbauensis (Apocynaceae): diversity and potential for L-asparaginase production. Braz J Microbiol 52, 1431–1441 (2021). https://doi.org/10.1007/s42770-021-00505-3

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