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Penicillium and Talaromyces endophytes from Tillandsia catimbauensis, a bromeliad endemic in the Brazilian tropical dry forest, and their potential for l-asparaginase production

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Abstract

This study was conducted to report the richness of endophytic Penicillium and Talaromyces species isolated from Tillandsia catimbauensis, a bromeliad endemic in the Brazilian tropical dry forest (Caatinga), to verify their ability to produce the enzyme l-asparaginase and to partially optimise the production of biomass and l-asparaginase of the best enzyme producer. A total of 184 endophytes were isolated, of which 52 (29%) were identified through morphological and phylogenetic analysis using β-tubulin sequences into nine putative species, four in Penicillium and five in Talaromyces. Talaromyces diversus and T. cf. cecidicola were the most frequent taxa. Among the 20 endophytic isolates selected for l-asparaginase production, 10 had the potential to produce the enzyme (0.50–2.30 U/g), especially T. cf. cecidicola URM 7826 (2.30 U/g) and Penicillium sp. 4 URM 7827 (1.28 U/g). As T. cf. cecidicola URM 7826 exhibited significant ability to produce the enzyme, it was selected for the partial optimisation of biomass and l-asparaginase production. Results of the 23 factorial experimental design showed that the highest dry biomass (0.66 g) was obtained under pH 6.0, inoculum concentration of 1 × 108 and 1% l-proline. However, the inoculum concentration was found to be statistically significant, the pH was marginally significant and the concentration of l-proline was not statistically significant. l-Asparaginase production varied between 0.58 and 1.02 U/g and did not reach the optimal point for enzyme production. This study demonstrates that T. catimbauensis is colonised by different Penicillium and Talaromyces species, which are indicated for enzyme production studies.

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Acknowledgements

We would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for financial support and scholarships. J.D.P. Bezerra also thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Finance code 001) and Fundação de Amparo à Ciência e Tecnologia de Pernambuco (FACEPE) for the postdoctoral fellowships. We are also grateful for the suggestions made by two anonymous reviewers. We extend our thanks to Aline Barboza, Ana P. Pádua, Tamara Caldas, Dr. Marília Maciel, and the students of the Laboratório de Micologia Ambiental/UFPE for their technical help and processing of samples.

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  1. Departamento de Micologia Prof. Chaves Batista, CB, Universidade Federal de Pernambuco, Av. Professor Nelson Chaves, s/n, Cidade Universitária, Recife, Pernambuco, CEP: 50670-901, Brazil

    Leticia F. Silva, Karla T. L. S. Freire, Minelli A. Sousa, Laura M. Paiva, Jadson D. P. Bezerra & Cristina M. Souza-Motta

  2. Programa de Pós Graduação em Biociência Animal, Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Rua Manoel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, CEP: 52171-900, Brazil

    Gianne R. Araújo-Magalhães

  3. Departamento de Estatística e Matemática Aplicada, Universidade Federal do Ceará, Av. Mister Hull, s/n, Pici, Fortaleza, Ceará, CEP: 60455-760, Brazil

    Gualberto S. Agamez-Montalvo

  4. Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580, B16, Cidade Universitária, São Paulo, SP, CEP: 05508-000, Brazil

    Tales A. Costa-Silva & Adalberto Pessoa-Junior

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Silva, L.F., Freire, K.T.L.S., Araújo-Magalhães, G.R. et al. Penicillium and Talaromyces endophytes from Tillandsia catimbauensis, a bromeliad endemic in the Brazilian tropical dry forest, and their potential for l-asparaginase production. World J Microbiol Biotechnol 34, 162 (2018). https://doi.org/10.1007/s11274-018-2547-z

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