Abstract
The objective of this research was to explore the candidate genes involved in calcium absorption of R. roxburghii plants, as well as their relationship with the bioactive substance accumulation in the fruit. RNA-seq and qRT-PCR were used to analyze the transcriptomic profiles of the roots and leaves of plants during calcium absorption. Based on the correlation between gene expression and calcium absorption rate, ion channels and carrier proteins were selected to further verify their response to exogenous calcium supplement and functions on the accumulation of ascorbate (AsA), flavonoid, and triterpenoid in the fruit. Transcriptome and qRT-PCR analysis revealed that a total of 25 candidate genes from seven families have been found to be closely associated with Ca2+ absorption, namely, cyclic nucleotide-gated ion channel (CNGC), zinc transporter (ZTP), metal tolerance protein (MTP), ER-type Ca2+ ATPase (ECA), auto-inhibited Ca2+ ATPase (ACA), glutamate receptor (GLR), and natural resistance-associated macrophage protein (NRAMP). Among these, RrCNGC1/7/10/12 and RrMTP2 play a vital role in responding to changes in external calcium levels. Moreover, exogenous Ca2+ promoted the accumulation of bioactive compounds such as AsA, total flavonoid, and total triterpenoid in fruits. RrCNGC2/3/12, RrZTP1, and RrGLR1 significantly responded to exogenous Ca2+ supplement, of which RrCNGC12 positively correlating with the levels of the above three types of bioactive substances. Seven families of ion channels and carrier proteins were jointly involved in calcium absorption and homeostasis in R. roxburghii plants, and RrCNGC12 play a critical role in the calcium-mediated regulation of fruit quality formation.
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Data Availability
All sequencing data are available through the NCBI Sequence Read Archive under the accession number PRJNA993289.
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This work was supported by the Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province (No. U1812401) and the National Natural Science Foundation of China (grant no. 32260730).
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Huaming An: conceptualization, methodology, formal analysis, writing — review and editing, visualization, supervision, funding acquisition. Min Lu: resources, validation, investigation. Zhao Wang: software, formal analysis, investigation, visualization, writing — original draft.
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Highlights
• We conducted experiments using Rosa roxburghii saplings as materials in three stages: Ca2+ starvation, rapid Ca2+ uptake, and Ca2+ saturation.
• We investigated the Ca2+ uptake patterns and molecular mechanisms in Rosa roxburghii plants.
• Some genes associated with Ca2+ uptake have an impact on fruit bioactive substance accumulation.
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Wang, Z., Lu, M. & An, H. Transcriptome Analysis Reveals Candidate Genes Involved in Calcium Absorption of Rosa roxburghii Plants and their Effects on the Bioactive Substance Accumulation in Fruit. J Soil Sci Plant Nutr 24, 732–748 (2024). https://doi.org/10.1007/s42729-023-01579-8
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DOI: https://doi.org/10.1007/s42729-023-01579-8