Abstract
The purpose of this work was to study the persistence of pesticides in different soil conditions (fallow, cultivated, and microbial inoculation) and their impact on the soil microbial diversity. The experiment was set up in pots under net house conditions. Using Biolog, substrate utilization patterns for community-level physiological profiling (CLPP) were carried out. The persistence of carbendazim in different treatments of fallow soil, maize rhizosphere, and plant parts with and without Trichoderma was studied using HPLC. The HPLC analysis of soil and maize tissue reveals increased persistence of carbendazim and its degraded products in fallow treatment compared to maize with or without Trichoderma. The application of Trichoderma enhanced the carbendazim removal and alleviated its effect on the microbial populations. The present study concludes that fallow conditions prolong the presence of residual pesticides in soil and affect the soil microbial community, whereas bio-fungicides such as Trichoderma facilitate its degradation.
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References
Aguilera J, Motavalli P, Valdivia C, Gonzales MA (2013) Impacts of cultivation and fallow length on soil carbon and nitrogen availability in the Bolivian Andean highland region. Mt Res Dev 33:391–404. https://doi.org/10.1659/MRD-JOURNAL-D-12-00077.1
Boudina A, Emmelin C, Baaliouamer A, Grenier-Loustalot MF, Chovelon JM (2003). Photochemical behavior of carbendazim in aqueous solution. Chemosphere 50(5):649–655, ISSN 0045–6535. https://doi.org/10.1016/S0045-6535(02)00620-3
Brussaard L, Ruiter P, Brown G (2007) Soil biodiversity for agricultural sustainability. Agric Ecosyst Environ 121:233–244. https://doi.org/10.1016/j.agee.2006.12.013
Chaudhary V, Rehman A, Mishra A, Chauhan PS, Nautiyal CS (2012) Changes in bacterial community structure of agricultural land due to long-term organic and chemical amendments. Microb Ecol 64:450–460. https://doi.org/10.1007/s00248-012-0025-y
Environmental Health Criteria 149 Carbendazim (1993) Geneva, International Programme on Chemical Safety, 132 pp
Garland JL (1996) Analytical approaches to the characterization of samples of microbial communities using patterns of potential C source utilization. Soil Biol Biochem 28:213–221. https://doi.org/10.1016/0038-0717(95)00112-3
Grogan HM, Jukes AA (2003) Persistence of the fungicides thiabendazole, carbendazim and prochloraz-Mn in mushroom casing soil. Pest Manag Sci 59(11):1225–1231. https://doi.org/10.1002/ps.759
Helweg A (1977) Degradation and adsorption of carbendazim and 2-aminobenzimidazole in soil. Pestic Sci 8:71–78. https://doi.org/10.1002/ps.2780080111
Jing-Liang Xu, Xiang-Yang Gu, Biao S, Zhi-Chun W, Kun W, Li S-P (2006) Isolation and characterization of a carbendazim-degrading Rhodococcus sp. djl-6. Curr Microbiol 53:72–76. https://doi.org/10.1007/s00284-005-0474-3
Kaiser D (2013) Soil fertility considerations and fallow syndrome. http://blog.lib.umn.edu/umnext/news/2013/10/soil-fertility.php
Kalwasinska A, Kesy J, Donderski W (2008) Biodegradation of carbendazim by epiphytic and neustonic bacteria of eutrophic Chelmzynskie lake. Pol J Microbiol 57:221–230. http://www.pjmonline.org/wp-content/uploads/2015/12/vol5732008221. Accessed 27 Sep 2011
Kumawat MK, Chandran NK, Sriram S (2019) Evaluation of carbendazim tolerant strain of Trichoderma harzianum GJ16B for its efficacy in the management of carbendazim resistant Fusarium solani isolate causing marigold wilt. Indian Phytopathol 1–6. https://doi.org/10.1007/s42360-019-00150-9
Lewandowska A, Walorczyk S (2010) Carbendazim residues in the soil and their bioavailability to plants in four successive harvests. Pol J Environ Stud 19(4):757–761
Meena RS, Kumar S, Datta R, Lal R, Vijayakumar V, Brtnicky M et al (2020) Impact of agrochemicals on soil microbiota and management: a review. Land 9(2):34. https://doi.org/10.3390/land9020034
Mishra A, Nautiyal CS (2009) Functional diversity of the microbial community in the rhizosphere of chickpea grown in diesel fuel spiked soil amended with Trichoderma ressei using sole-carbon source utilization profiles. World J Microbiol Biotechnol 25:1175–1180
Nautiyal CS, Chauhan PS, Bhatia CR (2010) Changes in soil physico-chemical properties and microbial functional diversity due to 14 years of conversion of grassland to organic agriculture in semi-arid agroecosystem. Soil Tillage Res 109(2):55–60. https://doi.org/10.1016/j.still.2010.04.008
Ons L, Bylemans D, Thevissen K, Cammue BPA (2020) Combining biocontrol agents with chemical fungicides for integrated plant fungal disease control. Microorganisms 8(12):1930. https://doi.org/10.3390/microorganisms8121930
Ramey BE, Koutsoudis M, Bodman SB, Fuqua C (2004) Biofilm formation in plant–microbe associations. Curr Opin Microbiol 7(6):602–609. https://doi.org/10.1016/j.mib.2004.10.014
Rasool S, Rasool T, Gani KM (2022) A review of interactions of pesticides within various interfaces of intrinsic and organic residue amended soil environment. Chem Eng J 11:2666–8211. https://doi.org/10.1016/j.ceja.2022.100301
Riemann L, Azam F (2002) Widespread N-acetyl-D-glucosamine uptake among pelagic marine bacteria and its ecological implications. Appl Environ Microbiol 68(11):5554–5562. https://doi.org/10.1128/AEM.68.11.5554-5562.2002
Singh PC, Nautiyal CS (2012) A novel method to prepare concentrated conidial biomass formulation of Trichoderma harzianum for seed application. Jappl Microbiol 113:1442–1450. https://doi.org/10.1111/j.1365-2672.2012.05426.x
Smith MD, Hartnett DC, Rice CW (2000) Effects of long-term fungicide applications on microbial properties in tall grass prairie soil. Soil Biol Biochem 32(7):935–946. https://doi.org/10.1016/S0038-0717(99)00223-0
Staddon WJ, Duchesne LC, Trevors JT (1997) Impact of clear-cutting and prescribed burning on microbial diversity and community structure in a Jack pine (Pinus Banksiana Lamb.) clear-cut using Biolog Gram-negative microplates. World J Microbiol Biotechnol 14(1):119–123. https://doi.org/10.1023/A:1008892921085
Tandon A, Fatima T, Gautam A, Yadav U, Srivastava S, Singh PC (2018) Effect of Trichoderma koningiopsis on chickpea rhizosphere activities under different fertilization regimes. Open J Soil Sci 8(10):261–275. https://doi.org/10.4236/ojss.2018.810020
Teufel R, Mascaraque V, Ismail W, Voss M, Perera J, Eisenreich W et al (2010) Bacterial phenylalanine and phenylacetate catabolic pathway revealed. Proc Natl Acad Sci 107(32):14390–14395. https://doi.org/10.1073/pnas.1005399107
Tian L, Chen F (2009) Characterization of a carbendazim-degrading Trichoderma sp. T2–2 and its application in bioremediation. Wei Sheng Wu Xue Bao, Acta Microbiol Sin 49(7):925–930
Tudi M, Daniel Ruan H, Wang L, Lyu J, Sadler R, Connell D, Chu C, Phung DT (2021) Agriculture development, pesticide application and its impact on the environment. Int J Environ Res 18(3):1112. https://doi.org/10.3390/ijerph18031112
Wang Z, Wang Y, Gong F, Zhang J, Hong Q, Li S (2010) Biodegradation of carbendazim by a novel Actinobacterium rhodococcus jialingiae djl-6-2. Chemosphere 81(5):639–644. https://doi.org/10.1016/j.chemosphere.2010.08.040
Yadav U, Bano N, Bag S, Srivastava S, Singh PC (2022) An insight into the endophytic bacterial community of tomato after spray application of propiconazole and Bacillus subtilis strain NBRI-W9. Microbiol Spectr 10(5):e01186–e1222. https://doi.org/10.1128/spectrum.01186-22
Yadav V, Panilaitis B, Shi H, Numuta K, Lee K, Kaplan DL (2011) N-acetylglucosamine 6-phosphate deacetylase (nagA) is required for N-acetyl glucosamine assimilation in Gluconaceto bacterxylinus. PLoS One 6(6). https://doi.org/10.1371/journal.pone.0018099
Yang C, Hamel C, Vujanovic V, Gan Y (2011) Fungicide: modes of action and possible impact on non-target microorganisms. ISRN Ecol 2011. https://doi.org/10.5402/2011/130289
Zhang Y, Wang H, Wang X, Hu B, Zhang C, Jin W, Zhu S, Hu G, Hong Q (2017) Identification of the key amino acid sites of the carbendazim hydrolase (MheI) from a novel carbendazim-degrading strain Mycobacterium sp. SD-4. J Hazard Mater 331:55–62. https://doi.org/10.1016/j.jhazmat.2017.02.007
Zubrod JP, Bundschuh M, Arts G, Brühl CA, Imfeld G, Knäbel A et al (2019) Fungicides: an overlooked pesticide class? Environ Sci Technol 53:3347–3365. https://doi.org/10.1021/acs.est.8b04392
Acknowledgements
We are grateful to the director of the CSIR-National Botanical Research Institute in Lucknow, India for providing all of the necessary facilities. We thank the ethical committee, CSIR-NBRI for evaluating the manuscript and providing MS No. CSIR-NBRI_MS/2023/02/05. Furthermore, we also thank Integral University for providing MCN no. IU/R&D/2022-MCN0001687.
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This study was supported by CSIR- National Botanical Research Institute's OLP-109 project and CSIR funded MLP049 project.
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All authors contributed to the study. Conceptualization: Dr. Poonam C. Singh, Soni Chauhan, and Touseef Fatima; methodology: SC and TF; writing and original draft preparation: SC, TF, and PCS; Biolog-related analysis by Dr. Puneet S. Chauhan; HPLC by TF, SC, and Om Prakash; review and editing: PCS, OP, and Amita Dubey; funding acquisition, resources, and supervision: PCS and PSC. All authors read and approved the final version of the manuscript.
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Touseef Fatima shares first authorship with Soni Chauhan.
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Standard curve prepared with the peak areas of 1, 5, and 10 μgml-1 carbendazim. (PNG 64 kb)
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Chauhan, S., Fatima, T., Dubey, A. et al. Integrated Application of Trichoderma and Carbendazim Affects the Carbendazim Extractability and Microbial Functions in the Maize Rhizosphere. J Soil Sci Plant Nutr 23, 3373–3380 (2023). https://doi.org/10.1007/s42729-023-01254-y
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DOI: https://doi.org/10.1007/s42729-023-01254-y