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Isolation and Characterization of a Carbendazim-Degrading Rhodococcus sp. djl-6

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Abstract

Bacterium djl-6, capable of degrading carbendazim, was isolated by continuous enrichment culture originating from carbendazim-treated soil. The isolate was identified as Rhodococcus sp. according to its phenotypic features, physiologic and biochemical characteristics, and phylogenetic analysis. The strain could use carbendazim as sole carbon or nitrogen source. It showed a high average degradation rate of 55.56 mg · L−1 · d−1 in M9 medium amended with carbendazim. High-pressure liquid chromatography–mass spectrometry (HPLC-MS) analysis showed the presence of 2-aminobenzimidazole, benzimidazole, and an unknown metabolite with molecular ions (M+) of m/z 104.8 and 118.5. The degradation in the isolate djl-6 seems to be initiated with the cleavage of the methyl carbemate side chain, resulting in the formation of 2-aminobenzimidazole and benzimidazole. This is the first report of the intermediates benzimidazole and 2-aminobenzimidazole found together in the culture filtrate of pure bacterium.

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Acknowledgments

This work was supported by the Chinese National Programs for High Technology Research and Development (Nos. 2003AA241150, 2004AA206071, and 2004AA214102) and the Programs for Science and Technology Development of Jiangsu Province (Nos. BE2002345 and BE2003343).

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Authors and Affiliations

  1. Ministry of Agriculture Key Laboratory of Microbiologic Engineering of Agricultural Environment, Nanjing Agricultural University, Nanjing, 210095, Peoples Republic of China

    Xu Jing-Liang, Gu Xiang-Yang, Shen Biao, Wang Zhi-Chun, Wang Kun & Li Shun-Peng

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  1. Xu Jing-Liang
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  2. Gu Xiang-Yang
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  3. Shen Biao
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  4. Wang Zhi-Chun
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  5. Wang Kun
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  6. Li Shun-Peng
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Correspondence to Li Shun-Peng.

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Jing-Liang, X., Xiang-Yang, G., Biao, S. et al. Isolation and Characterization of a Carbendazim-Degrading Rhodococcus sp. djl-6. Curr Microbiol 53, 72–76 (2006). https://doi.org/10.1007/s00284-005-0474-3

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  • DOI: https://doi.org/10.1007/s00284-005-0474-3

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