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The potential use of Eichhornia crassipes (Mart.) Solms against Agrotis ipsilon (Hufn.) (Lepidotera: Noctuidae), its efficacy as an insecticide on vital biological activities and assessment on its safety

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Abstract

The current study was carried out to evaluate eco-friendly extracts of an invasive weed; Eichhornia crassipes (Mart.) Solms (Pontederiaceae) for controlling Agrotis ipsilon (Hufn.) (Lepidotera: Noctuidae). The insecticidal and biological activities of the whole plant extracts with different polarity solvents (petroleum ether, acetone, and absolute ethanol) were investigated. The extracts were evaluated against the 2nd larval instars of A. ipsilon. The results revealed that acetone fraction extract was the most effective extract with the lowest LC50 value (240 mg/L). The larval mortality percentage increased by elevating concentrations of all tested extracts. Pupation and adult emergence percentages were inversely proportional with the concentration of all tested extracts. There was a disturbance in both the adult malformation and sex ratios. Furthermore, the spectrophotometric testing of the most promising extracts (acetone and ethanol fractions) on some biochemical parameters (total body metabolites, acid and alkaline phosphatases, aminotransferase activities, and kidney function) in the serum of albino rats showed non-toxic effects on mammals. Therefore, acetone and ethanol fraction extracts of E. crassipes were considered promising eco-friendly insecticidal alternatives in (Integrated Pest Management) IPM programs to control A. ipsilon worldwide.

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References

  • Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267

    Article  CAS  Google Scholar 

  • Abdel-Monaim MF (2013) Improvement of biocontrol of damping-off and root rot/wilt of faba bean by salicylic acid and hydrogen peroxide. Mycobiology 41:47–55. https://doi.org/10.5941/MYCO.2013.41.1.47

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • AbdelKhalek ST (2018) Recycle Waste of Plant Origin to Insecticides for Controlling Field Crop Pests in New Valley Governorate. M.Sc. Thesis. Ain Shams Univ., Cairo, Egypt

  • AbdelKhalek ST, Mostafa ZK, Hassan HA, et al (2018) A new list to the entomofauna associated with faba bean, Vicia faba L. (Fabales: Fabaceae) grown in El-Kharga Oasis, New Valley Governorate, Egypt. Egypt Acad J Biol Sci A, Entomol 11:105–110. https://doi.org/10.21608/eajb.2018.11901

  • Adebo CT, Adeyemi JA, Adedire CO (2018) Biochemical and histopathological effects of a bioinsecticide, Anchomanes difformis (Blume) Engler rhizome powder on Wistar rats. Comp Clin Path 27:1545–1550. https://doi.org/10.1007/s00580-018-2771-9

    Article  CAS  Google Scholar 

  • Adeyemi OT, Osilesi O, Adebawo OO et al (2015) Alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) Activities in selected tissues of rats fed on processed atlantic horse mackerel (Trachurus trachurus). Adv Biosci Biotechnol 6:139–152. https://doi.org/10.4236/abb.2015.63014

    Article  Google Scholar 

  • Ahmed MAI, Temerak SAH, Abdel-Galil FAK, Manna SHM (2016) Susceptibility of field and laboratory strains of Cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) to spinosad pesticide under laboratory conditions. Plant Prot Sci 52:128–133. https://doi.org/10.17221/5/2015-PPS

  • Allotey J, Azalekor W (2000) Some aspects of the biology and control using botanicals of the rice moth, Corcyra cephalonica (Stainton), on some pulses. J Stored Prod Res 36:235–243. https://doi.org/10.1016/S0022-474X(99)00045-4

    Article  CAS  PubMed  Google Scholar 

  • Al Zarog A (2008) Oral Administrated Pesticides Residual Activity On Rat Kidney Function. J Sebha Univ Appl Sci 7:5–13

    Google Scholar 

  • American Psychological Association (2016) Revision of Ethical Standard 3.04 of the “Ethical Principles of Psychologists and Code of Conduct” (2002) as amended 2010). Am Psychol 71:900. https://doi.org/10.1037/amp0000102

    Article  Google Scholar 

  • Arivoli S, Tennyson S (2012) Antifeedant activity of plant extracts against Spodoptera litura (Fab.)(Lepidoptera: Noctuidae). Am J Agric Environ Sci 12:764–768. https://doi.org/10.5829/idosi.aejaes.2012.12.06.63178

    Article  Google Scholar 

  • Atangwho IJ, Ebong PE, Eyong EU et al (2009) Comparative chemical composition of leaves of some antidiabetic medicinal plants: Azadirachta indica, Vernonia amygdalina and Gongronema latifolium. African J Biotechnol 8:4685–4689. https://doi.org/10.5897/AJB09.082

    Article  CAS  Google Scholar 

  • Belfield A, Goldberg DM (1971) Revised assay for serum phenyl phosphatase activity using 4-amino-antipyrine. Enzyme 12:561–573. https://doi.org/10.1159/000459586

    Article  CAS  PubMed  Google Scholar 

  • Bermúdez-Torres K, Ae J, Martínez H et al (2009) Activity of quinolizidine alkaloids from three Mexican Lupinus against the lepidopteran crop pest Spodoptera frugiperda. BioContro 54:459–466. https://doi.org/10.1007/s10526-008-9180-y

    Article  CAS  Google Scholar 

  • Buncharoen W, Saenphet S, Chomdej S, Saenphet K (2012) Evaluation of biochemical, hematological and histopathological parameters of albino rats treated with Stemona aphylla Craib. extract. J Med Plants Res 6:4429–4435. https://doi.org/10.5897/JMPR12.595

    Article  Google Scholar 

  • Couto IFS, Souza SA, Valente FI et al (2020) Changes in the Biological Characteristics of Plutella xylostella Using Ethanolic Plant Extracts. Gesunde Pflanz 72:383–391. https://doi.org/10.1007/s10343-020-00520-8

    Article  CAS  Google Scholar 

  • Dahi H, Ibrahem W, Ali M (2009) Heat Requirements for the Development of the Black cutworm, Agrotis ipsilon (Hüfnagel) (Noctuidae: Lepidoptera). Egypt Acad J Biol Sci A, Entomol 2:117–124. https://doi.org/10.21608/eajbsa.2009.15502

  • Derbalah AS, Hamza AM, Gazzy AA (2012) Efficacy and safety of some plant extracts as alternatives for Sitophilus oryzae control in rice grains. J Entomol 9:57–67. https://doi.org/10.3923/je.2012.57.67

    Article  CAS  Google Scholar 

  • Ebadollahi A, Safaralizadeh M, Pourmirza A, Gheibi S (2010) Toxicity of essential oil of Agastache foeniculum (Pursh) Kuntze to Oryzaephilus surinamensis L. and Lasioderma serricorne F. J Plant Prot Res 50:215–219. https://doi.org/10.2478/v10045-010-0037-x

    Article  CAS  Google Scholar 

  • Ebeid AR (2020) The efficiency of some plant extracts against Agrotis ipsilon (Lepidoptera: Noctuidae) regarding to their activity on vital biochemical parameters. GSC Biol Pharm Sci 12:240–248. https://doi.org/10.30574/gscbps.2020.12.1.0229

  • El-Beih NM, Ramadan G, Khorshed MA, Ahmed RSA (2017) Biochemical alterations in insecticides-treated male albino rats: potential modulatory effects of a standardized aged garlic extract. Indian J Tradit Knowl 16:181–188

    Google Scholar 

  • Elhadek MK, Mohamady AH, Ali RE (2015) Toxicity and Biochemical Effects of Four Plant Essential Oils Against Cotton Leafworm, Spodoptera littoralis (Boisd). Egypt Acad J Biol Sci F Toxicol Pest Control 7:153–162. https://doi.org/10.21608/eajbsf.2015.17250

  • Fawcett JK, Scott JE (1960) A rapid and precise method for the determination of urea. J Clin Pathol 13:156–159. https://doi.org/10.1136/jcp.13.2.156

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fetoh BE-SA, Asiry KA (2012) Toxicological and larvicidal activities of Alzanzalakhet, Melia azedarach against cucurbit fly, Dacus ciliatus at Hail Province in Saudi Arabia. Toxicol Environ Chem 94:1350–1356. https://doi.org/10.1080/02772248.2012.705466

    Article  CAS  Google Scholar 

  • Finney DJ (1971) Probit Analysis, 3rd edn. Cambridge University Press, London, UK

  • Gameel SMM (2014) Effect of planting date on seasonal abundance of the Aphis craccivora koch and the associated predators in the new Valley-Egypt. Egypt Acad J Biol Sci A, Entomol 7:97–103. https://doi.org/10.21608/eajbsa.2014.13141

  • Gamil WE, Mariy FM, Youssef LA, Abdel Halim SM (2011) Effect of Indoxacarb on some biological and biochemical aspects of Spodoptera littoralis (Boisd.) larvae. Ann Agric Sci 56:121–126. https://doi.org/10.1016/j.aoas.2011.07.005

    Article  Google Scholar 

  • Gnanamani R, Dhanasekaran S (2017) Efficacy of azadirachta indica leaf extract on the biochemical estimation of a lepidopteran pestPericallia ricini (Lepidoptera: Arctiidae). World J Agric Sci 13:63–67. https://doi.org/10.5829/idosi.wjas.2017.63.67

    Article  CAS  Google Scholar 

  • Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177:751–766

    Article  CAS  Google Scholar 

  • Govindarajan M, Mathivanan T, Elumalai K et al (2011) Ovicidal and repellent activities of botanical extracts against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi (Diptera: Culicidae). Asian Pac J Trop Biomed 1:43–48. https://doi.org/10.1016/S2221-1691(11)60066-X

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Guna V, Ilangovan M, Anantha Prasad MG, Reddy N (2017) Water Hyacinth: A Unique Source for Sustainable Materials and Products. ACS Sustain Chem Eng 5:4478–4490. https://doi.org/10.1021/acssuschemeng.7b00051

    Article  CAS  Google Scholar 

  • Guo Y, Qu H, Zhi X, et al (2013) Semisynthesis and Insecticidal Activity of Some Fraxinellone. J Agric Food Chem 61:11937–11944. https://doi.org/10.1021/jf404195m. Epub

  • Harold V (1975) Practical Clinical Biochemistry; 4th ed.; Arnold-Heinemann Publishers (India) Private Limited: India

  • Hassan HA (2013) The effect of water hyacinth, Eichhornia crassipes, and King-Bo compound on some biological and biochemical aspects of cotton leaf worm Spodoptera littoralis (Boisd). Egypt Acad J Biol Sci 5:33–45. https://doi.org/10.21608/EAJBSF.2013.17270

  • Hatem AE, Abdel-Samad SSM, Saleh HA et al (2009) Toxicologyical and physiological activity of plant extracts against Spodoptera littoralis (Boisduval)(Lepidoptera: Noctuidae) larvae. Bol Sanid Veg Plagas 35:517–531

    Google Scholar 

  • Ibrahim SRH (2012) Combined effect of gamma radiation and some plant ex-tracts on Spodoptera littoralis. Benha University, Qalubia, Egypt, Ph.D

    Google Scholar 

  • Jayanthi P, Lalitha P, Aarthi N (2012) Larvicidal and pupicidal activity of extracts and fractionates of Eichhornia crassipes (Mart.) Solms against the filarial vector Culex quinquefasciatus Say. Parasitol Res 111:2129–2135. https://doi.org/10.1007/s00436-012-3061-0

    Article  CAS  PubMed  Google Scholar 

  • Khazanie R (1979) Elementary statistics. Goodyear Pub. Co., Santa Monica Calif

    Google Scholar 

  • Kind P, King E (1954) Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol 7:322–326

    Article  CAS  Google Scholar 

  • Kirst HA (2010) The spinosyn family of insecticides: realizing the potential of natural products research. J Antibiot (tokyo) 63:101–111. https://doi.org/10.1038/ja.2010.5

    Article  CAS  Google Scholar 

  • Lalitha P, Sripathi SK, Jayanthi P (2012) Acute toxicity study of extracts of Eichhornia Crassipes (Mart.) Solms. Asian J Pharm Clin Res 5:59–61

    Google Scholar 

  • Mabrouk MSO, Mahbob MA-M (2017) Survey of the Most Common Insect Species on Some Foraging Crops of Honeybees in Dakhla Oasis, New Valley Governorate, Egypt. J Ecol Heal Environ 5:35–40. https://doi.org/10.18576/jehe/050105

  • Mesbah HA, H. T, Mourad AK, et al (2020) Silica Nano Particles Bait Against the Black Cutworm, Agrotis Ipsilon (Hufn.) Physico-Chemical and Organolyptical Characteristics Of Cake Fortified By Irradiated Broccoli (Brassica Oleracea L.Var Italica) Powder. J Nucl Technol Appl Sci 6:153–162. https://doi.org/10.21608/jntas.2020.38203.1026

  • Nwosu LC, Adedire CO, Ogunwolu EO, Ashamo MO (2017) Toxicological and histopathological effects of Dennettia tripetala seed used as grain protectant, food, and medicine. Food Qual Saf 1:211–220. https://doi.org/10.1093/fqsafe/fyx019Published:07August2017

    Article  CAS  Google Scholar 

  • Odeyemi OO, Masika P, Afolayan AJ (2008) A review of the use of phytochemicals for insect pest control. African Plant Prot 14:1–7

    Google Scholar 

  • Rani PU, Jamil K (1989) Effect of water hyacinth leaf extract on mortality, growth and metamorphosis of certain pests of stored products. Int J Trop Insect Sci 10:327–332. https://doi.org/10.1017/S1742758400003568

    Article  Google Scholar 

  • Rapoport A, Husdan H (1968) Endogenous creatinine clearance and serum creatinine in the clinical assessment of kidney function. Can Med Assoc J 99:149–156

    CAS  PubMed  PubMed Central  Google Scholar 

  • Reda FA, Gniedy NA, Saadiya M et al (2006) Occurrence of the biological effect of some plant extracts on the cotton leaf worm Spodoptera littoralis-(Boisd) and their physiological impact. J Egypt Acad Soc Environ Dev 7:109–147

    Google Scholar 

  • Robinson GS, Ackery PR, Kitching IJ, et al (2010) HOSTS-a database of the world’s Lepidopteran hostplants. Nat. Hist. Museum, London

  • Sabbour MM, Abd El-Aziz S (2002) Efficacy of some botanical oils formulated with microbial agents against the cotton leafworm and greasy cutworm attaching cotton plants. Bull Entomol Soc Egypt, Econ Ser 28:135–151

    Google Scholar 

  • Saki N, Saki G, Rahim F, Nikakhlagh S (2011) Modulating effect of soy protein on serum cardiac enzymes in cholesterol-fed rats. Int J Med Med Sci 3:390–395. https://doi.org/10.5897/IJMMS.9000155

    Article  CAS  Google Scholar 

  • Saxena RC, Dixit OP, Sukumaran P (1992) Laboratory assessment of indigenous plant extracts for anti-juvenile hormone activity in Culex quinquefasciatus. Indian J Med Res 95:204. https://doi.org/10.1007/s13197-010-0049-6. Epub

  • Sharaby A, El-Nujiban A (2013) Biological Activity of Essential Oil of Sage Plan Leaves Salvia offecinalis L. against the Black Cutworm Agrotis ipsilon (Hubn.). Int J Sci Res 4:2319–7064

    Google Scholar 

  • Sharaby AMF, Gesraha MA, Fallatah SAB (2020) Botanical extracts against the potato tuber moth, Phthorimaea operculella (Zeller 1873) (Lepidoptera: Gelechiidae), during storage conditions. Egypt J Biol Pest Control 30:1–6. https://doi.org/10.1186/s41938-020-00294-1

    Article  Google Scholar 

  • Sharma A, Sharma MK, Kumar M (2007) Protective effect of Mentha piperita against arsenic-induced toxicity in liver of Swiss Albino mice. Basic Clin Pharmacol Toxicol 100:249–257. https://doi.org/10.1111/j.1742-7843.2006.00030.x

    Article  CAS  PubMed  Google Scholar 

  • Simmonds MSJ (2003) Flavonoid-insect interactions: Recent advances in our knowledge. Phytochemistry 64:21–30. https://doi.org/10.1016/S0031-9422(03)00293-0

    Article  CAS  PubMed  Google Scholar 

  • Sullivan PR, Wood R (2012) Water hyacinth (Eichhornia crassipes (Mart.) Solms) seed longevity and the implications for management. In: Eighteenth Australasian Weeds Conference. pp 37–40

  • Trinder P (1969) Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. J Clin Pathol 22:158–161. https://doi.org/10.1136/jcp.22.2.158

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zama D, Meraihi Z, Boubekri N et al (2005) Assessment of the changes in some diagnostics enzymes and other parameters in wistar albinorats treated with pesticides during gestation. Sci Technol c, Biotechnol 23:51–56

    Google Scholar 

  • Zöllner N, Kirsch K (1962) Colorimetric Method for Determination of Total Lipids. Journal of Experimental Medicine. Z Gesamte Exp Med 135:545–550. https://doi.org/10.1007/BF02045455

    Article  Google Scholar 

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Acknowledgements

The present work was supported jointly by the Academy of Scientific Research and Technology (ASRT), Cairo, Egypt and the National Key Research and Development Program of China. The authors gratefully acknowledge their support.

Funding

The present work was financially supported by the National Key Research and Development Program of China (2018YFC1200400).

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Authors and Affiliations

  1. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Sara Taha Abdelkhalek & Man-Qun Wang

  2. Department of Entomology, Faculty of Science, Ain Shams University, Cairo, 11566, Egypt

    Sara Taha Abdelkhalek, Zahia Kamel Mostafa & Marah Mohammad Abd El-Bar

  3. Plant Protection Research Institute, Agricultural Research Center, Giza, Egypt

    Heba Abd El-Wahab Hassan

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  1. Sara Taha Abdelkhalek
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  2. Zahia Kamel Mostafa
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  3. Heba Abd El-Wahab Hassan
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  4. Man-Qun Wang
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  5. Marah Mohammad Abd El-Bar
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Contributions

Conceptualization, Sara Taha Abdelkhalek and Heba Abd El-Wahab Hassan; validation, Sara Taha Abdelkhalek, Marah Mohamed Abd El-Bar, and Zahia Kamel Mostafa; investigation, Sara Taha Abdelkhalek and Man-Qun Wang; data curation, Sara Taha Abdelkhalek, Heba Abd El-Wahab Hassan, and Zahia Kamel Mostafa; writing—original draft preparation, Sara Taha Abdelkhalek and Marah Mohamed Abd El-Bar; writing—review and editing, Man-Qun Wang and Marah Mohamed Abd El-Bar.; supervision, Zahia Kamel Mostafa and Man-Qun Wang; funding acquisition, Man-Qun Wang.

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Correspondence to Man-Qun Wang.

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Ethical approval was waived by the local Ethics Committee (Research Ethics Committee (REC) for Animal Subject Research) of Ain Shams University, Egypt (Ethics approval number:18467) in view of the retrospective nature of the study.

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Abdelkhalek, S.T., Mostafa, Z.K., Hassan, H.A.EW. et al. The potential use of Eichhornia crassipes (Mart.) Solms against Agrotis ipsilon (Hufn.) (Lepidotera: Noctuidae), its efficacy as an insecticide on vital biological activities and assessment on its safety. Int J Trop Insect Sci 42, 741–753 (2022). https://doi.org/10.1007/s42690-021-00598-1

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