Abstract
Bitter gourd plants exhibiting various kinds of symptoms were collected in famer’s fields in Karnataka and Uttar Pradesh states of India. The casual agent associated with symptomatic bitter gourd plants (One sample) was artificially inoculated through whiteflies (Bemisia tabaci) to the healthy bitter gourd plants. Further host range studies indicate that it will infects few plant species in the Cucurbitaceae family. The status of the virus associated with bitter gourd plants was assesses by subjecting the total DNA of 80 symptomatic samples to PCR using begomovirus specific primers. The resulted PCR amplicon of 1.2 kb partial viral genome sequencing and analysis indicates that 80 symptomatic bitter gourd samples are infected by begomovirus, which is closely related to tomato leaf curl New Delhi virus (ToLCNDV). The representative bitter gourd samples were selected to amplify the complete viral genome of eight isolates using RCA method. The pair wise nucleotide sequence analysis of DNA-A (nucleotide identity of 92.8–97.6%) and DNA-B (nucleotide identity of 82.9–95.1%) of eight bitter gourd isolates showed more homology with ToLCNDV affecting cucurbits, lentil, chilli, tomato, bitter gourd and pumpkin. The recombination break point analysis of the complete genome of eight bitter gourd isolates indicates these isolates are exchange the DNA fragments in both the components with already prevailing begomoviruses. The cryptic species of the whiteflies identified as Asia-I and Asia-II-5 group, which were collected from infected bitter gourd fields. The significance of these research findings is discussed.
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References
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410. https://doi.org/10.1016/S0022-2836(05)80360-2
Arguello-Astorga GR, Ruiz-Medrano R (2001) An iteron-related domain is associated to motif 1 in the replication proteins of geminiviruses: identification of potential interacting amino acid-base pairs by a comparative approach. Arch Virol 146:1465–1485. https://doi.org/10.1007/s007050170072
Arguello-Astorga GR, Guevara-Gonzalez LR, Herrera-Estrella LR, Rivera-Bustamante RF (1994) Geminivirus replication origins have a group-specific organization of iterative elements: a model for replication. Virology 203:90–100. https://doi.org/10.1006/viro.1994.1458
Ashfaq M, Hebert PDN, Mirza MS, Khan AM, Mansoor S, Shah GS, Zafar Y (2014) DNA barcoding of Bemisia tabaci complex (Hemiptera: aleyrodidae) reveals southerly expansion of the dominant whitefly species on cotton in Pakistan. PLoS One. https://doi.org/10.1371/journal.pone.0104485
Ashwathappa KV, Venkataravanappa V, Reddy CNL, Reddy MK (2019) Association of Tomato leaf curl New Delhi virus with mosaic and leaf curl disease of chrysanthemum and its whitefly cryptic species. Indian Phytopathol 73:533–542. https://doi.org/10.1007/s42360-020-00214-1
Briddon RW, Bull SE, Mansoor S, Amin I, Markham PG (2002a) Universal primers for the PCR-mediated amplification of DNA beta: a molecule associated with some monopartite begomoviruses. Mol Biotech 20:315–318. https://doi.org/10.1385/MB:20:3:315
Briddon RW, Bull SE, Mansoor S, Amin I, Markham PG (2002b) Universal primers for the PCR-mediated amplification of DNA-B eta: a molecule associated with some monopartite begomoviruses. Mol Biotechnol 20:315–318. https://doi.org/10.1385/MB:20:3:315
Briddon RW, Bull SE, Amin I, Mansoor S, Bedford ID, Rishi N, Siwatch SS, Zafar Y, Abdel-Salam AM, Markham PG (2004) Diversity of DNA 1: a satellite-like molecule associated with monopartite begomovirus–DNA β complexes. Virology 324:462–474. https://doi.org/10.1016/j.virol.2004.03.041
Chatterji A, Chatterji U, Beachy RN, Fauquet CM (2000) Sequence parameters that determine specificity of binding of the replication-associated protein to its cognate site in two strains of Tomato leaf curl virus-New Delhi. Virology 273:341–350. https://doi.org/10.1006/viro.2000.0434
Enikuomehin OA, Peters OT (2002) Evaluation of crude extracts from some Nigerian plants for the control of field diseases of sesame (Sesamum indicum L.) Tropical Oilseeds J 7: 84–93
Fortes IM, Sanchez-Campos S, Fiallo-Olive E, Diaz-Pendón JA, Navas-Castillo J, Moriones E (2016) A novel strain of tomato leaf curl New Delhi virus has spread to the Mediterranean basin. Viruses 8(11):307. https://doi.org/10.3390/v8110307
Garg ID, Paul-Khurana SM, Kumar S, Lakra BS (2001) Association of a geminivirus with potato apical leaf curl in India and its immunoelectron microscopic detection. J Indain Potato Assoc 28:227–232
Gray SM, Banerjee N (1999) Mechanisms of arthropod transmission of plant and animal viruses. Microbiol Mol Biol Rev 63:128–148. https://doi.org/10.1128/MMBR.63.1.128-148.1999
Hanley-BowDoin L, Settleage SB, Orozco BM, Nagar S, Robertson D (1999) Geminivirus: models for plant DNA replication, transcription, and cell cycle regulation. Crit Rev Plant Sci 18:71–106. https://doi.org/10.1080/07352689991309162
Heyraud F, Matzeit V, Kammann M, Schafer S, Schell J, Gronenborn B (1993) Identification of the initiation sequence for viral-strand DNA synthesis of wheat dwarf virus. EMBO J 12:4445–4452. https://doi.org/10.1002/j.1460-2075.1993.tb06130.x
Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23:254–267. https://doi.org/10.1093/molbev/msj030
Juarez M, Rabadan MP, Martínez LD, Tayahi M, Grande-Perez A, Gomez P (2019) Natural hosts and genetic diversity of the emerging tomato leaf curl New Delhi virus in Spain. Front Microbiol 10:140. https://doi.org/10.3389/Fmicb.2019.00140
Khan MS, Raj SK, Singh R (2006) First report of tomato leaf curl New Delhi virus infecting chilli in India. Plant Pathol 55:289. https://doi.org/10.1111/j.1365-3059.2006.01324.x
Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096
Lozano G, Trenado HP, Fiallo-Olivé E, Chirinos D, Geraud-Pouey F, Briddon RW, Navas-Castillo J (2016) Characterization of non-coding DNA satellites associated with sweepoviruses (genus Begomovirus, Geminiviridae)—definition of a distinct class of begomovirus-associated satellites. Front Microbiol 7:162. https://doi.org/10.3389/fmicb.2016.00162
Mandal B, Mandal S, Sohrab SS, Pun KB, Varma A (2004) A new yellow mosaic disease of chayote in India. Plant Pathol 53:797. https://doi.org/10.1111/j.1365-3059.2004.01075.x
Manivannan K, Renukadevi P, Malathi VG, Karthikeyan G, Balakrishnan N (2019) A new seed-transmissible begomovirus in bitter gourd (Momordica charantia L.). Microb Pathog 128:82–89. https://doi.org/10.1016/j.micpath.2018.12.036
Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evol 1:vev003. https://doi.org/10.1093/ve/vev003
Moriones E, Praveen S, Chakraborty S (2017) Tomato leaf curl New Delhi virus: an emerging virus complex threatening vegetable and fiber crops. Viruses 9(10):264. https://doi.org/10.3390/V9100264
Muhire BM, Varsani A, Martin DP (2014) SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9(9):e108277. https://doi.org/10.1371/journal.pone.0108277
Nagendran K, Satya VK, Mohan Kumar S, Karthikeyan G (2016) Molecular characterization of a distinct bipartite Begomovirus species infecting ivy gourd (Coccinia grandis L.) in Tamil Nadu, India. Virus Genes 52:146–151. https://doi.org/10.1007/s11262-015-1278-6
Orozco BM, Gladfelter HJ, Settlage SB, Eagle PA, Gentry RN, Hanley-BowDoin L (1998) Multiple cis-elements contribute to geminivirus origin function. Virology 242:346–356. https://doi.org/10.1006/viro.1997.9013
Prasanna HC, Rai M (2007) Detection and frequency of recombination in tomato-infecting begomoviruses of South and Southeast Asia. Virol J 4:111. https://doi.org/10.1186/1743-422X-4-111
Raj SK, Khan MS, Singh R, Nisha K, Prakash D (2005) Occurrence of yellow mosaic geminiviral disease on bitter gourd (Momordica charantia) and its impact on phytochemical contents. Int J Food Sci Nutr 56:185–192. https://doi.org/10.1071/DN10006
Raj SK, Snehi KS, Khan MS, Tiwari A, Rao G (2010) First report of pepper leaf curl Bangladesh virus strain associated with bitter gourd (Momordica charantia L.) yellow mosaic disease in India. Australas Plant Dis Notes 5:14–16. https://doi.org/10.1071/DN10006
Reddy CRV, Colvin J, Muniyappa V, Seal S (2005) Diversity and distribution of begomoviruses infecting tomato in India. Arch Virol 150:845–867. https://doi.org/10.1007/s00705-004-0486-5
Reddy CRV, Kirankumar M, Seal SE, Muniyappa V, Girish B, Valand, Govindappa, MR, Colvin J (2012) Bemisia tabaci phylogenetic groups in India and the relative transmission efficacy of tomato leaf curl Bangalore virus by an indigenous and an exotic population. J Integr Agric 11:235–248. https://doi.org/10.1016/S2095-3119(12)60008-2
Rekha AR, Maruthi MN, Muniyappa V, Colvin J (2005) Occurrence three genotypic clusters of B. tabaci and the rapid spread of B-biotype in south India. Entomol Exp Appl 117:221–233. https://doi.org/10.1111/J.1570-7458.2005.00352.X
Roumagnac P, Lett JM, Fiallo-Olivé E et al (2022) Establishment of five new genera in the family Geminiviridae: citlodavirus, maldovirus, mulcrilevirus, opunvirus, and topilevirus. Arch Virol 167:695–710. https://doi.org/10.1007/s00705-021-05309-2
Shankarappa KS, Rangaswamy KT, Aswatha Narayana DS, Rekha AR, Raghavendra N, Lakshminarayana Reddy CN, Chancellor TCB, Maruthi MN (2007) Development of silverleaf assay, protein and nucleic acid-based diagnostic techniques for the quick and reliable detection and monitoring of biotype B of the whitefly, Bemisia tabaci (Gennadius). Bull Entomol Res 97:503–513. https://doi.org/10.1017/S0007485307005251
Sohrab SS, Mandal B, Pant RP, Varma A (2003) First reports of association of tomato leaf curl New Delhi virus with yellow mosaic disease of Luffa cylindrica in India. Plant Dis 87:1148. https://doi.org/10.1094/PDIS.2003.87.9.1148A
Sohrab SS, Mandal B, Ali A, Varma A (2010) Chlorotic curly stunt: a severe begomovirus disease of bottle gourd in Northern India. Indian J Virol 21:56–63. https://doi.org/10.1007/s13337-010-0002-3
Sunkad G, Nagoji B, Srinivasaraghavan A (2012) Survey for the incidence and sources of field resistance against peanut bud necrosis disease of groundnut in north eastern Karnataka. The Bioscan 7(3):387–390
Tiwari AK, Sharma PK, Khan MS, Snehi SK, Raj SK, Rao GP (2010) Molecular detection and identification of tomato leaf curl New Delhi virus isolate causing yellow mosaic disease in Bitter gourd (Momordica charantia), a medicinally important plant in India. Med Plants 2(2):117–123. https://doi.org/10.5958/j.0975-4261.2.2.018
Varsani A, Roumagnac P, Fuchs M, Navas-Castillo J, Moriones E, Idris A, Briddon RW, Rivera-Bustamante R, Murilo Zerbini F, Martin DP (2017) Capulavirus and Grablovirus: two new genera in the family Geminiviridae. Arch Virol 162:1819–1831. https://doi.org/10.1007/s00705-017-3268-6
Venkataravanappa V, Reddy CNL, Jalali S, Reddy MK (2012) Molecular characterization of distinct bipartite begomovirus infecting bhendi (Abelmoschus esculentus L.) in India. Virus Genes 44:522–535. https://doi.org/10.1007/s11262-012-0732-y
Venkataravanappa V, Prasanna HC, Reddy CNL, Reddy MK (2014) Evidence for two predominant viral lineages, recombination and subpopulation structure in begomoviruses associated with yellow vein mosaic disease of okra in India. Plant Pathol 64(3):508–518. https://doi.org/10.1111/ppa.12292
Venkataravanappa V, Swarnalatha P, Reddy CNL, Neha Chauhan, Krishna Reddy M (2016) Association of recombinant Chilli leaf curl virus with enation leaf curl disease of tomato: a new host for chilli begomovirus in India. Phytoparasitica 44:213–223
Venkataravanappa V, Kodandaram MH, Lakshminarayana Reddy CN, Shankarappa KS, Krishna Reddy M (2017) Comparative transmission of bhendi yellow vein mosaic virus by two cryptic species of the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). 3 Biotech 7:331. https://doi.org/10.1007/s13205-017-0970-8
Venkataravanappa V, Reddy CNL, Shankarappa KS, Jayappa J, Pandey S, Krishna Reddy M (2019) Characterization of Tomato leaf curl New Delhi virus and DNA- satellites association with mosaic disease of cucumber. Int J Biotech Bioeng 5(6):93–109
Venkataravanappa V, Reddy CNL, Nandan M, Shridhar H, Ashwathappa KV, Shankarappa KS, Vinay Kumar HD, Reddy MK (2021) Transmission, characterization and occurrence of recombination in Indian strain of squash leaf curl China virus associated with yellow mosaic and leaf curl disease of Summer squash. 3 Biotech 11:265. https://doi.org/10.1007/s13205-021-02821-9
Walker PJ, Siddell SG, Lefkowitz EJ et al (2020) Changes to virus taxonomy and thes ratified by the International Committee on Taxonomy of Viruses. Arch Virol 165:2737–2748. https://doi.org/10.1007/s00705-020-04752-x
Zaidi SSEA, Martin DP, Amin I, Farooq M, Mansoor S (2017) Tomato leaf curl New Delhi virus: a widespread bipartite begomovirus in the territory of monopartite begomoviruses. Mol Plant Pathol 18(7):901–911. https://doi.org/10.1111/Mpp.12481
Zerbini FM, Briddon RW, Idris A, Martin DP, Moriones E, Navas-Castillo J, Rivera-Bustamante R, Roumagnac P, Varsani A, Ictv Report C (2017) ICTV virus taxonomy profile: Geminiviridae. J Gen Virol 98:131–133. https://doi.org/10.1099/jgv.0.000738
Zhang QC (1992) Bitter melon: a herb warranting a closer look. PWA Coalition Newsline 81:48–49
Acknowledgements
The authors are grateful to the Director, ICAR-Indian Institute of Horticultural Research, Bengaluru, for providing research facilities and his keen interest in this study.
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We declare that all authors participated in the research and article preparation. ON worked and designed the experiments and drafted the article. VV and CNLR designed the study and revised the manuscript and MKR participated in analysis of the data. AKC and VV guided and designed the study and revised the article. All authors approved the submitted version of the article.
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Naik, S.O., Venkataravanappa, V., Chakravarthy, A.K. et al. Survey, host range and characterization of begomovirus infecting bitter gourd and its vector whitefly cryptic species. Indian Phytopathology 75, 1111–1127 (2022). https://doi.org/10.1007/s42360-022-00544-2
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DOI: https://doi.org/10.1007/s42360-022-00544-2