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Molecular characterization of distinct bipartite begomovirus infecting bhendi (Abelmoschus esculentus L.) in India

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Abstract

Yellow vein mosaic disease of okra is a whitefly transmitted begomovirus causing heavy economic loss in different parts of India. The okra isolate (OY131) of this virus from a bhendi plant [(Abelmoschus esculentus L.) Moench] showing yellow vein mosaic, vein twisting, reduced leaves, and a bushy appearance in the Palem region, New Delhi, India, was characterized in the present study. The complete DNA-A and DNA-B sequences have been determined and are comprised of 2,746 and 2,703 nucleotides, respectively. The betasatellite (DNA-β) component was absent in the sample. The genome organization was typically of biparite begomoviruses, which were characterized earlier. Comparison of DNA-A component with other known begomoviruses suggest that this virus, being only distantly related (<85.9% similarity with its nearest relative, BYVMV) to other known begomoviruses, is a new species. We have tentatively assigned the genome to a novel geminivirus species Bhendi yellow vein mosaic Delhi virus [BYVDV-IN (India: Delhi: okra)]. DNA-B showed highest sequence identity (87.8% identical) to that of a ToLCNDV (AY158080). The phylogenetic analysis of the present isolate is distinct from all other viruses; however clusters with ToLCNDV group infect different crops. The recombination analysis revealed that this isolate has sequences originated from ToLCNDV. This is the first known bhendi yellow vein mosaic disease associated bipartite begomovirus from India.

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Acknowledgments

The research was supported by ICAR NETWORK project on development of diagnostics to emerging plant viruses, Indian Council of Agricultural Research, Government of India, New Delhi. We thank DP Martin, Computational Biology Group, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa for going through the manuscript and suggesting the corrections.

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Authors and Affiliations

  1. Division of Plant Pathology, Plant Virology Laboratory, Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore, 560 089, India

    V. Venkataravanappa, Salil Jalali & M. Krishna Reddy

  2. Indian Institute of Vegetable Research, Varanasi, 221305, Uttar Pradesh, India

    V. Venkataravanappa

  3. Department of Plant Pathology, College of Sericulture, UAS (B), Chintamani, Kolar, 563125, India

    C. N. Lakshminarayana Reddy

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  1. V. Venkataravanappa
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  2. C. N. Lakshminarayana Reddy
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  3. Salil Jalali
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  4. M. Krishna Reddy
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Correspondence to M. Krishna Reddy.

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11262_2012_732_MOESM1_ESM.jpg

S1 Detection of BYVDV in the total DNA isolated from naturally infected bhendi samples by dot blot hybridization using nonradioactive digoxginin labelled Probe of betasatellite of Bhendi yellow vein Dehli virus (the dot circle depicts the +ve betasatellite clone). (JPEG 4 kb)

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Venkataravanappa, V., Lakshminarayana Reddy, C.N., Jalali, S. et al. Molecular characterization of distinct bipartite begomovirus infecting bhendi (Abelmoschus esculentus L.) in India. Virus Genes 44, 522–535 (2012). https://doi.org/10.1007/s11262-012-0732-y

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  • DOI: https://doi.org/10.1007/s11262-012-0732-y

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