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Stem Cell Banking of Adipose Tissue

  • Artificial Tissues (A Atala and J G Hunsberger, Section Editors)
  • Published:
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Abstract

Purpose of Review

Adult stem cells such as adipose tissue derived cells (ASCs) are often preferred for autologous cellular therapies. Nevertheless, their regenerative potential is vulnerable to donor conditions (age and disease status) as well as in vitro culture conditions (contamination, in vitro passaging). These donor- and culture-related variables compromise stem cell efficacy when used to treat and cure various diseases. Cryopreservation of stem cells may solve these problems by offering cryogenic preservation of healthy cells and tissues for future use.

Recent Findings

Establishment of stem cell banks to preserve tissues and stem cells is growing rapidly. Adipose tissue is a unique therapeutic option for patients in that it can be used not only as a “whole tissue” but also as a source of cells for regenerative medicine applications. Adipose tissue contains the highest number of mesenchymal stem cells (MSCs), called ASCs, per tissue weight. Adipose tissue has gained clinical interest for use in regenerative medicine because it is easily accessible and readily available, contains large numbers of stem cells, and is economical to utilize. However, optimal adipose tissue is not always available for autologous use when needed; therefore, cryopreservation could be an alternative approach to make it available for donors when needed. Stem cell banks have been developed to offer greater flexibility of use of adipose tissue and ASCs for biomedical research and future clinical applications.

Summary

Adipose tissue is routinely discarded during various surgical procedures and could be cryopreserved as “whole tissue” or as “cells” (i.e., ASCs). Stem cell banks may enhance the safety and efficacy of adipose tissue and ASC use for future cellular therapies. In addition, stem cell banks may make cell-based therapies more effective and economical for patients.

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References

Papers of particular interest, published recently, have been highlighted as: • Of importance •• Of major importance

  1. Harris DT. Stem cell banking for regenerative and personalized medicine. Biomedicines. 2014;2(1):50–79.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Choudhery MS, Badowski M, Muise A, Pierce J, Harris DT. Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation. J Transl Med. 2014;12:8.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Mirabet V, Solves P. Stem cell banking. Regen Nephrol. 2011. https://doi.org/10.1016/B978-0-12-380928-5.10026-0.

    Article  Google Scholar 

  4. • Choudhery MS. Strategies to improve regenerative potential of mesenchymal stem cells. World J Stem Cells. 2021;13(12):1845–62. Advanced donor age as well as donor health condition has a profound effect on regenerative potential of stem cells for autologous cell therapy. The review had discussed various strategies that can enhance the compromised regenerative potential of cells.

    Article  PubMed  PubMed Central  Google Scholar 

  5. Haque N, Abu Kasim NH. Pooled human serum increases regenerative potential of in vitro expanded stem cells from human extracted deciduous teeth. Adv Exp Med Biol. 2018;1083:29–44.

    Article  PubMed  CAS  Google Scholar 

  6. Brooke G, Cook M, Blair C, Han R, Heazlewood C, Jones B, et al. Therapeutic applications of mesenchymal stromal cells. Semin Cell Dev Biol. 2007;18:846–58.

    Article  PubMed  CAS  Google Scholar 

  7. Thirumala S, Goebel WS, Woods EJ. Clinical grade adult stem cell banking. Organogenesis. 2009;5(3):143–54.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Choudhery MS, Harris DT. Cryopreservation can be used as an anti-aging strategy. Cytotherapy. 2014;16(12):1771–3.

    Article  PubMed  Google Scholar 

  9. Fu X, Xu B, Jiang J, Du X, Yu X, Yan Y, Li S, Inglis BM, Ma H, Wang H, Pei X, Si W. Effects of cryopreservation and long-term culture on biological characteristics and proteomic profiles of human umbilical cord-derived mesenchymal stem cells. Clin Proteomics. 2020;17:15.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  10. Choudhery MS, Badowski M, Muise A, Pierce J, Harris DT. Cryopreservation of whole adipose tissue for future use in regenerative medicine. J Surg Res. 2014;187(1):24–35.

    Article  PubMed  CAS  Google Scholar 

  11. •• Kamenaga T, Kuroda Y, Nagai K. Cryopreserved human adipose-derived stromal vascular fraction maintains fracture healing potential via angiogenesis and osteogenesis in an immunodeficient rat model. Stem Cell Res Ther. 2021;12:110. The therapeutic effect of human stromal vascular fraction (SVF) cells have been evaluated on fracture healing in a rat non-healing fracture model. In addition, the therapeutic effects of freshly isolated and cryopreserved SVFs have been compared.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  12. Vakhshori V, Bougioukli S, Sugiyama O, Tang A, Yoho R, Lieberman JR. Cryopreservation of human adipose-derived stem cells for use in ex vivo regional gene therapy for bone repair. Hum Gene Ther Methods. 2018;29(6):269–77.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  13. Pegg DE. Principles of cryopreservation. Methods Mol Biol. 2007;368:39–57.

    Article  PubMed  CAS  Google Scholar 

  14. Shu Z, Heimfeld S, Gao D. Hematopoietic SCT with cryopreserved grafts: adverse reactions after transplantation and cryoprotectant removal before infusion. Bone Marrow Transplant. 2014;49(4):469–476.

  15. Crowe JH, Crowe LM, Oliver AE, Tsvetkova N, Wolkers W, Tablin F. The trehalose myth revisited: introduction to a symposium on stabilization of cells in the dry state. Cryobiology. 2001;43(2):89–105.

    Article  PubMed  CAS  Google Scholar 

  16. Capicciotti CJ, Kurach JD, Turner TR, Mancini RS, Acker JP, Ben RN. Small molecule ice recrystallization inhibitors enable freezing of human red blood cells with reduced glycerol concentrations. Sci Rep. 2015;5:9692.

    Article  PubMed  PubMed Central  Google Scholar 

  17. Sputtek A, Sputtek R. Cryopreservation in transfusion medicine and hematology. In: Fuller BJ, Lane N, Benson EE, editors. Life in the Frozen State. Boca Raton: CRC Press; 2004. p. 483–504.

    Chapter  Google Scholar 

  18. Hunt CJ. Cryopreservation of human stem cells for clinical application: a review. Transfus Med Hemother. 2011;38(2):107–23.

    Article  PubMed  PubMed Central  Google Scholar 

  19. Karow AM Jr. Cryoprotectants–a new class of drugs. J Pharm Pharmacol. 1969;21(4):209–23.

    Article  PubMed  CAS  Google Scholar 

  20. Choudhery MS, Badowski M, Muise A, Pierce J, Harris DT. Subcutaneous adipose tissue derived stem cell utility is independent of anatomical harvest site. Biores Open Access. 2015 Feb 1;4(1):131–45. https://doi.org/10.1089/biores.2014.0059. PMID: 26309790; PMCID: PMC4497709.

  21. Li K, Gao J, Zhang Z, Li J, Cha P, Liao Y, Wang G, Lu F. Selection of donor site for fat grafting and cell isolation. Aesthetic Plast Surg. 2013 Feb;37(1):153-8. https://doi.org/10.1007/s00266-012-9991-1. Epub 2012 Dec 12. PMID: 23232729.

  22. Lim AA, Fan K, Allam KA. Autologous fat transplantation in the craniofacial patient. J Craniofac Surg. 2012;23(4):1061–1066.

  23. Choudhery MS, Badowski M, Muise A, Harris DT. Utility of cryopreserved umbilical cord tissue for regenerative medicine. Curr Stem Cell Res Ther. 2013;8(5):370–380.

  24. Abu-Ghname A, Perdanasari AT, Reece EM. Principles and applications of fat grafting in plastic surgery. Semin Plast Surg. 2019;33(3):147–54.

    Article  PubMed  PubMed Central  Google Scholar 

  25. Bashir MM, Sohail M, Ahmad FJ, Choudhery MS. Preenrichment with adipose tissue-derived stem cells improves fat graft retention in patients with contour deformities of the face. Stem Cells Int. 2019;2019:5146594.

    Article  PubMed  PubMed Central  Google Scholar 

  26. • Bashir A, Bashir MM, Sohail M, Choudhery MS. Adipose tissue grafting improves contour deformities related hyperpigmentation of face. J Craniofac Surg. 2020;31(5):1228–31. An innovative technique to simultaneously heal the contour deformity as well as to remove hyperpigmentation has been used in the current study.

    PubMed  Google Scholar 

  27. Vriend L, van Dongen JA, Pijpe A, Nieuwenhuis MK, Jongen SJM, Harmsen MC, van Zuijlen PPM, van der Lei B. Stromal vascular fraction-enriched fat grafting as treatment of adherent scars: study design of a non-randomized early phase trial. Trials. 2022;23(1):575.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  28. Zhang J, Liu Y, Chen Y, Yuan L, Liu H, Wang J, Liu Q, Zhang Y. Adipose-derived stem cells: current applications and future directions in the regeneration of multiple tissues. Stem Cells Int. 2020;2020:8810813.

    Article  PubMed  PubMed Central  Google Scholar 

  29. Fan BS, Liu Y, Zhang JY, Chen YR, Yang M, Yu JK. Principles for establishment of the stem cell bank and its applications on management of sports injuries. Stem Cell Res Ther. 2021;12(1):307.

    Article  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Human Genetics & Molecular Biology, University of Health Sciences, Lahore, Pakistan

    Mahmood S Choudhery

  2. Allama Iqbal Medical College/Jinnah Hospital, University of Health Sciences, Lahore, Pakistan

    Ruhma Mahmood

  3. Department of Immunobiology and University of Arizona Health Sciences Biorepository, College of Medicine, University of Arizona, Tucson, Arizona, USA

    David T. Harris

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  1. Mahmood S Choudhery
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Correspondence to Mahmood S Choudhery.

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Choudhery, M.S., Mahmood, R. & Harris, D.T. Stem Cell Banking of Adipose Tissue. Curr Stem Cell Rep 8, 174–183 (2022). https://doi.org/10.1007/s40778-022-00222-z

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