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Characterization of Biofilm Bacterial Communities in a Vertical Unsaturated-Flow Bioreactor Treating Domestic Greywater

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Abstract

Greywater (GW) is wastewater generated from domestic activities without the input of toilet and kitchen. Its reuse can help to reduce the overall potable water consumption in urban areas. In the current study, an extensive greywater treatment unit consisting of an attached growth unsaturated-flow bio-reactor, with “BIOROCK”® medium, was developed. Its performance was studied and the bacterial communities of the BIOROCK® biofilm were characterized through 454-pyrosequencing of 16S rRNA genes. The medium physical properties (porosity and high specific surface) made it possible to support highly diverse and relatively long-term stable bacterial communities that showed spatial variations in their relative abundance. The bacterial communities were dominated by genera previously associated with wastewater environments and that present some kind of degrading activities. Consequently, after treatment, water turbidity was reduced by 83 % and COD removal efficiency was 80 %, demonstrating that most of the biodegradable matter had been removed. Some pathogens were detected, but usually at low abundances. With some minor adjustments and the addition of a chlorination step, the treated greywater generated by this system can be used for non-potable purposes, such as irrigation and toilet flushing.

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References

  • Adrados B, Sánchez O, Arias CA, Becares E, Garrido L, Mas J, Brix H, Morató J (2014) Microbial communities from different types of natural wastewater treatment systems: vertical and horizontal flow constructed wetlands and biofilters. Water Res 55:304–312

    Article  Google Scholar 

  • APHA, AWWA, WEF (2005) Standard methods for the examination of water and wastewater. https://www.standardmethods.org/. Accessed 22 Jun 2015

  • Aslam Z, Im WT, Ten LN, Lee ST (2005) Phenylobacterium koreense sp. nov., isolated from South Korea. Int J Syst Evol Microbiol 55(Pt 5):2001–2005

    Article  Google Scholar 

  • Bai HJ, Zhang ZM, Yang GE, Li BZ (2008) Bioremediumtion of cadmium by growing Rhodobacter sphaeroides: kinetic characteristic and mechanism studies. Bioresour Technol 99(16):7716–7722

    Article  Google Scholar 

  • Bailey MT, Walton JC, Dowd SE, Weil ZM, Nelson RJ (2010) Photoperiod modulates gut bacteria composition in male Siberian Hamsters (Phodopus sungorus). Brain Behav Immun 24(4):577–84

    Article  Google Scholar 

  • Blanky M, Rodríguez-Martínez S, Halpern M, Friedler E (2015) Legionella pneumophila: from potable water to treated greywater; quantification and removal during treatment. Sci Total Environ 533:557–565

    Article  Google Scholar 

  • Caporaso JG, Bittinger K, Bushman FD, DeSantis TZ, Andersen GL, Knight R (2010a) PyNAST: a flexible tool for aligning sequences to a template alignment. Bioinformatics 26(2):266–267

    Article  Google Scholar 

  • Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK et al (2010b) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7(5):335–336

    Article  Google Scholar 

  • Cheng KC, Demirci A, Catchmark JM (2010) Advances in biofilm reactors for production of value-added products. Appl Microbiol Biotechnol 87(2):445–456

    Article  Google Scholar 

  • Dalahmeh SS, Jonsson H, Hylander LD, Hui N, Yu D, Pell M (2014) Dynamics and functions of bacterial communities in bark, charcoal and sand filters treating greywater. Water Res 54:21–32

    Article  Google Scholar 

  • Edgar RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26(19):2460–2461

    Article  Google Scholar 

  • Eriksson E, Auffarth K, Henze M, Ledin A (2002) Characteristics of grey wastewater. Urban Water J 4(1):85–104

    Article  Google Scholar 

  • Fields BS (1996) The molecular ecology of legionellae. Trends Microbiol 4(7):286–290

    Article  Google Scholar 

  • Friedler E (2008) The water saving potential and the socio-economic feasibility of greywater reuse within the urban sector – Israel as a case study. Int J Environ Stud 65(1):57–69

    Article  Google Scholar 

  • Friedler E, Gilboa Y (2010) Performance of UV disinfection and the microbial quality of greywater effluent along a reuse system for toilet flushing. Sci Total Environ 408(9):2109–2117

    Article  Google Scholar 

  • Friedler E, Kovalio R, Galil NI (2005) On-site greywater treatment and reuse in multi-storey buildings. Water Sci Technol 51(10):187–194

    Google Scholar 

  • Friedler E, Kovalio R, Ben-Zvi A (2006) Comparative study of the microbial quality of greywater treated by three on-site treatment systems. Environ Technol 27(6):653–663

    Article  Google Scholar 

  • Friedler E, Yardeni A, Gilboa Y, Alfiya Y (2011) Disinfection of greywater effluent and regrowth potential of selected bacteria. Water Sci Technol 63(5):931–940

    Article  Google Scholar 

  • Gilboa Y, Friedler E (2008) UV disinfection of RBC-treated light greywater effluent: kinetics, survival and regrowth of selected microorganisms. Water Res 42(4-5):1043–1050

    Article  Google Scholar 

  • Harmsen M, Yang L, Pamp SJ, Tolker-Nielsen T (2010) An update on Pseudomonas aeruginosa biofilm formation, tolerance, and dispersal. FEMS Immunol Med Mic 59(3):253–268

    Article  Google Scholar 

  • Hruska K, Kaevska M (2012) Mycobacteria in water, soil, plants and air: a review. Vet Med 57(12):623–679

    Google Scholar 

  • Hu M, Wang X, Wen X, Xia Y (2012) Microbial community structures in different wastewater treatment plants as revealed by 454-pyrosequencing analysis. Bioresour Technol 117:72–79

    Article  Google Scholar 

  • Inbar Y (2007) New standards for treated wastewater reuse in Israel. In: Zaidi MK (ed) Wastewater reuse: risk assessment, decision-making and environmental security. Part of the NATO Science for Peace and Security Series. Springer, Netherlands, pp 291–296

    Google Scholar 

  • Ke N, Xiao C, Ying Q, Ji S (2003) A new species of the genus Phenylobacterium for the degradation of LAS (linear alkylbenzene sulfonate). Acta Microbiol Sin 43(1):1–7 (in Chinese)

    Google Scholar 

  • Keely SP, Brinkman NE, Zimmerman BD, Wendell D, Ekeren KM, De Long SK, Sharvelle S, Garland JL (2015) Characterization of the relative importance of human- and infrastructure-associated bacteria in grey water: a case study. J Appl Microbiol 119:289–301

    Article  Google Scholar 

  • Kim MK, Park MJ, Im WT, Yang DC (2008) Aeromicrobium ginsengisoli sp. nov., isolated from a ginseng field. Int J Syst Evol Microbiol 58(Pt 9):2025–2030

    Article  Google Scholar 

  • Levenspiel O (1999) Chemical reaction engineering. Wiley, New York

    Google Scholar 

  • Li P, Wang X, Stagnitti F, Li L, Gong Z, Zhang H et al (2005) Degradation of phenanthrene and pyrene in soil slurry reactors with immobilized bacteria Zoogloea sp. Environ Eng Sci 22(3):390–399

    Article  Google Scholar 

  • Li F, Wichmann K, Otterpohl R (2009) Review of the technological approaches for grey water treatment and reuses. Sci Total Environ 407(11):3439–3449

    Article  Google Scholar 

  • MacRae JD, Smit J (1991) Characterization of Caulobacters isolated from wastewater treatment systems. Appl Environ Microbiol 57(3):751–758

    Google Scholar 

  • Makropoulos CK, Butler D (2010) Distributed water infrastructure for sustainable communities. Water Resour Manag 24:2795–2816

    Article  Google Scholar 

  • Mara D, Horan N (2003) Handbook of water and wastewater microbiology, 1st edn. Academic, Cambridge

    Google Scholar 

  • Markelova NY (2010) Predacious bacteria, Bdellovibrio with potential for biocontrol. Int J Hyg Environ Health 213(6):428–431

    Article  Google Scholar 

  • Metcalf L, Eddy HP (2002) Wastewater engineering: treatment and reuse. McGraw-Hill Education, Columbus

    Google Scholar 

  • Molmeret M, Horn M, Wagner M, Santic M, Abu Kwaik Y (2005) Amoebae as training grounds for intracellular bacterial pathogens. Appl Environ Microbiol 71(1):20–28

    Article  Google Scholar 

  • Moore ERB, Tindall BJ, Santos VAPM, Pieper DH, Ramos J, Palleron NJ (2006) Nonmedical: Pseudomonas. In: Dworkin et al (eds) The Prokaryotes, 3rd edn. Springer, New York, pp 646-703

  • Pitta DW, Pinchak E, Dowd SE, Osterstock J, Gontcharova V, Youn E et al (2010) Rumen bacterial diversity dynamics associated with changing from bermudagrass hay to grazed winter wheat diets. Microb Ecol 59(3):511–522

    Article  Google Scholar 

  • Reinhold-Hurek B, Hurek T (2006) The Genera Azoarcus, Azovibrio, Azospira and Azonexus. In: Dworkin et al (eds) The Prokaryotes, 3rd edn. Springer, New York, pp 873-891

  • Resmi G, Thampi SG, Chandrakaran S (2010) Brevundimonas vesicularis: a novel bio-sorbent for removal of lead from wastewater. Int J Environ Res 4(2):281–288

    Google Scholar 

  • Revetta RP, Santo Domingo JW, Kelty C, Humrighouse B, Oerther D, Lamendella R et al (2007) Molecular diversity of drinking water microbial communities: a phylogenetic approach. Proc Water Environ Fed 1:629–645

    Article  Google Scholar 

  • Rodríguez-Martinez S, Sharaby Y, Brettar I, Pecellin M, Höfle M, Halpern M (2015) Spatial distribution of Legionella pneumophila MLVA-genotypes in a drinking water system. Water Res 77:119–132

    Article  Google Scholar 

  • Rodríguez-Martínez S, Blanky M, Friedler E, Halpern M (2015) Legionella spp. isolation and quantification from greywater. Methods X 2:458–462

    Google Scholar 

  • Ryu SH, Park M, Lee JR, Yun PY, Jeon CO (2007) Brevundimonas aveniformis sp. nov., a stalked species isolated from activated sludge. Int J Syst Evol Microbiol 57(Pt 7):1561–1565

    Article  Google Scholar 

  • Sawayama S, Krishna Rao K, Hall DO (1998) Immobilization of Rhodobacter capsulatus on cellulose beads and water treatment using a photobioreactor. J Ferment Bioeng 86(5):517–520

    Article  Google Scholar 

  • Stamper DM, Walch M, Jacobs RN (2003) Bacterial population changes in a membrane bioreactor for greywater treatment monitored by denaturing gradient gel electrophoretic analysis of 16S rRNA gene fragments. Appl Environ Microbiol 69(2):852–860

    Article  Google Scholar 

  • Steinert M, Heuner K, Buchrieser C, Albert-Weissenberger C, Glöckner G (2007) Legionella pathogenicity: genome structure, regulatory networks and the host cell response. Int J Med Microbiol 297(7-8):577–587

    Article  Google Scholar 

  • Stumm W, Morgan JJ (1996) Aquatic chemistry: chemical equilibria and rates in natural waters. Wiley, New Jersey

    Google Scholar 

  • Sun H, Shi B, Bai Y, Wang D (2014) Bacterial community of biofilm developed under different water supply conditions in a distribution system. Sci Total Environ 472:99–107

    Article  Google Scholar 

  • Thrash JC, Pollock J, Torok T, Coates JD (2010) Description of the novel perchlorate-reducing bacteria Dechlorobacter hydrogenophilus gen. nov., sp. nov. and Propionivibrio militaris, sp. nov. Appl Microbiol Biotechnol 86(1):335–343

    Article  Google Scholar 

  • Wagner M, Loy A (2002) Bacterial community composition and function in sewage treatment systems. Curr Opin Biotechnol 13(3):218–227

    Article  Google Scholar 

  • Wang Q, Garrity GM, Tiedje JM, Cole JR (2007) Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73(16):5261–5267

    Article  Google Scholar 

  • Ward NL, Challacombe JF, Janssen PH, Henrissat B, Coutinho PM, Wu M, Xie G et al (2009) Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils. Appl Environ Microbiol 75(7):2046–2056

    Article  Google Scholar 

  • Yoon JH, Kang SJ, Ryu SH, Jeon CO, Oh TK (2008) Hydrogenophaga bisanensis sp. nov., isolated from wastewater of a textile dye works. Int J Syst Evol Microbiol 58(Pt 2):393–397

    Article  Google Scholar 

  • Zhang Y, Grant A, Sharma A, Chen D, Chen L (2010) Alternative water resources for rural residential development in Western Australia. Water Resour Manag 24:25–36

    Article  Google Scholar 

  • Zhang T, Shao MF, Ye L (2012) 454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants. ISME J 6(6):1137–1147

    Article  Google Scholar 

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Acknowledgments

This study was supported by the Technion–University of Haifa (Israel) joint research foundation and by a grant from the German Research Foundation (DFG, the Deutsche Forschungsgemeinschaft) GZ: HO 930/5-2. The authors thank Jonathan Trow for his help in depositing the sequences at the National Center for Biotechnology Information (NCBI).

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Authors and Affiliations

  1. Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, Haifa, Israel

    Sara Rodríguez-Martínez, Yana Aizenberg-Gershtein, Yehonatan Sharaby & Malka Halpern

  2. Department of Environmental, Water & Agricultural Engineering, Faculty of Civil and Environmental Engineering, Technion, Haifa, Israel

    Anat Dekel, Yael Gilboa & Eran Friedler

  3. Department of Biology and Environment, Faculty of Natural Sciences, University of Haifa, Oranim, Tivon, Israel

    Malka Halpern

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  1. Sara Rodríguez-Martínez
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  2. Anat Dekel
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  5. Yehonatan Sharaby
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Correspondence to Malka Halpern.

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Rodríguez-Martínez, S., Dekel, A., Aizenberg-Gershtein, Y. et al. Characterization of Biofilm Bacterial Communities in a Vertical Unsaturated-Flow Bioreactor Treating Domestic Greywater. Environ. Process. 3, 325–340 (2016). https://doi.org/10.1007/s40710-016-0162-2

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