Tarnopolsky M. Protein requirements for endurance athletes. Nutrition (Burbank, Los Angeles County, Calif). 2004;20(7–8):662–8.
Lemon PW, Mullin JP. Effect of initial muscle glycogen levels on protein catabolism during exercise. J Appl Physiol Respir Environ Exerc Physiol. 1980;48(4):624–9.
Thomas DT, Erdman KA, Burke LM. American College of Sports Medicine Joint Position Statement. Nutrition and Athletic Performance. Med Sci Sports Exerc. 2016;48(3):543–68.
Tayrose GA, Beutel BG, Cardone DA, Sherman OH. The masters athlete: a review of current exercise and treatment recommendations. Sports health. 2015;7(3):270–6.
Mitchell WK, Williams J, Atherton P, Larvin M, Lund J, Narici M. Sarcopenia, dynapenia, and the impact of advancing age on human skeletal muscle size and strength; a quantitative review. Front Physiol. 2012;3:260.
Fleg JL, Morrell CH, Bos AG, Brant LJ, Talbot LA, Wright JG, et al. Accelerated longitudinal decline of aerobic capacity in healthy older adults. Circulation. 2005;112(5):674–82.
Lazarus NR, Harridge SDR. Declining performance of master athletes: silhouettes of the trajectory of healthy human ageing? J Physiol. 2017;595(9):2941–8.
McKendry J, Breen L, Shad BJ, Greig CA. Muscle morphology and performance in master athletes: a systematic review and meta-analyses. Ageing Res Rev. 2018;45:62–82.
Piasecki J, Ireland A, Piasecki M, Deere K, Hannam K, Tobias J, et al. Comparison of muscle function, bone mineral density and body composition of early starting and later starting older masters athletes. Front Physiol. 2019;10:1050.
Hawkins SA, Wiswell RA, Marcell TJ. Exercise and the master athlete—a model of successful aging? J Gerontol Ser A. 2003;58(11):M1009–11.
Churchward-Venne TA, Holwerda AM, Phillips SM, van Loon LJ. What is the optimal amount of protein to support post-exercise skeletal muscle reconditioning in the older adult? Sports Med (Auckland, NZ). 2016;46(9):1205–12.
Murphy CH, Oikawa SY, Phillips SM. Dietary protein to maintain muscle mass in aging: a case for per-meal protein recommendations. J Frailty Aging. 2016;5(1):49–58.
Doherty TJ. Invited review: aging and sarcopenia. J Appl Physiol (Bethesda, Md : 1985). 2003;95(4):1717–27.
Narici MV, Maffulli N. Sarcopenia: characteristics, mechanisms and functional significance. Br Med Bull. 2010;95(1):139–59.
Joanisse S, Nederveen JP, Snijders T, McKay BR, Parise G. Skeletal muscle regeneration, repair and remodelling in aging: the importance of muscle stem cells and vascularization. Gerontology. 2017;63(1):91–100.
Cuthbertson D, Smith K, Babraj J, Leese G, Waddell T, Atherton P, et al. Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle. FASEB J. 2005;19(3):422–4.
Moore DR, Churchward-Venne TA, Witard O, Breen L, Burd NA, Tipton KD, et al. Protein ingestion to stimulate myofibrillar protein synthesis requires greater relative protein intakes in healthy older versus younger men. J Gerontol A Biol Sci Med Sci. 2015;70(1):57–62.
Katsanos CS, Kobayashi H, Sheffield-Moore M, Aarsland A, Wolfe RR. A high proportion of leucine is required for optimal stimulation of the rate of muscle protein synthesis by essential amino acids in the elderly. Am J Physiol Endocrinol Metab. 2006;291(2):E381–7.
Yang Y, Breen L, Burd NA, Hector AJ, Churchward-Venne TA, Josse AR, et al. Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men. Br J Nutr. 2012;108(10):1780–8.
Robinson MJ, Burd NA, Breen L, Rerecich T, Yang Y, Hector AJ, et al. Dose-dependent responses of myofibrillar protein synthesis with beef ingestion are enhanced with resistance exercise in middle-aged men. Appl Physiol Nutr Metab. 2013;38(2):120–5.
Witard OC, Jackman SR, Breen L, Smith K, Selby A, Tipton KD. Myofibrillar muscle protein synthesis rates subsequent to a meal in response to increasing doses of whey protein at rest and after resistance exercise. Am J Clin Nutr. 2014;99(1):86–95.
Kim IY, Schutzler S, Schrader A, Spencer HJ, Azhar G, Ferrando AA, et al. The anabolic response to a meal containing different amounts of protein is not limited by the maximal stimulation of protein synthesis in healthy young adults. Am J Physiol Endocrinol Metab. 2016;310(1):E73-80.
Moore DR, Robinson MJ, Fry JL, Tang JE, Glover EI, Wilkinson SB, et al. Ingested protein dose response of muscle and albumin protein synthesis after resistance exercise in young men. Am J Clin Nutr. 2009;89(1):161–8.
Volpi E, Mittendorfer B, Wolf SE, Wolfe RR. Oral amino acids stimulate muscle protein anabolism in the elderly despite higher first-pass splanchnic extraction. Am J Physiol. 1999;277(3):E513–20.
Gorissen SHM, Trommelen J, Kouw IWK, Holwerda AM, Pennings B, Groen BBL, et al. Protein type, protein dose, and age modulate dietary protein digestion and phenylalanine absorption kinetics and plasma phenylalanine availability in humans. J Nutr. 2020;150(8):2041–50.
Pennings B, Groen BB, van Dijk JW, de Lange A, Kiskini A, Kuklinski M, et al. Minced beef is more rapidly digested and absorbed than beef steak, resulting in greater postprandial protein retention in older men. Am J Clin Nutr. 2013;98(1):121–8.
Rémond D, Machebeuf M, Yven C, Buffière C, Mioche L, Mosoni L, et al. Postprandial whole-body protein metabolism after a meat meal is influenced by chewing efficiency in elderly subjects. Am J Clin Nutr. 2007;85(5):1286–92.
Needleman I, Ashley P, Fine P, Haddad F, Loosemore M, de Medici A, et al. Oral health and elite sport performance. Br J Sports Med. 2015;49(1):3–6.
van Wijck K, Lenaerts K, van Loon LJ, Peters WH, Buurman WA, Dejong CH. Exercise-induced splanchnic hypoperfusion results in gut dysfunction in healthy men. PloS One. 2011;6(7):e22366.
van Wijck K, Pennings B, van Bijnen AA, Senden JM, Buurman WA, Dejong CH, et al. Dietary protein digestion and absorption are impaired during acute postexercise recovery in young men. Am J Physiol Regul Integr Comp Physiol. 2013;304(5):R356–61.
Mazzulla M, Parel JT, Beals JW, Vanv S, Abou Sawan S, West DWD, et al. Endurance exercise attenuates postprandial whole-body leucine balance in trained men. Med Sci Sports Exerc. 2017;49(12):2585–92.
Frontera WR, Hughes VA, Fielding RA, Fiatarone MA, Evans WJ, Roubenoff R. Aging of skeletal muscle: a 12-yr longitudinal study. Journal of applied physiology (Bethesda, Md: 1985). 2000;88(4):1321–6.
Mitchell WK, Phillips BE, Williams JP, Rankin D, Smith K, Lund JN, et al. Development of a new Sonovue™ contrast-enhanced ultrasound approach reveals temporal and age-related features of muscle microvascular responses to feeding. Physiol Rep. 2013;1(5):e00119.
Timmerman KL, Lee JL, Dreyer HC, Dhanani S, Glynn EL, Fry CS, et al. Insulin stimulates human skeletal muscle protein synthesis via an indirect mechanism involving endothelial-dependent vasodilation and mammalian target of rapamycin complex 1 signaling. J Clin Endocrinol Metab. 2010;95(8):3848–57.
Timmerman KL, Lee JL, Fujita S, Dhanani S, Dreyer HC, Fry CS, et al. Pharmacological vasodilation improves insulin-stimulated muscle protein anabolism but not glucose utilization in older adults. Diabetes. 2010;59(11):2764–71.
Hepple RT, Mackinnon SL, Goodman JM, Thomas SG, Plyley MJ. Resistance and aerobic training in older men: effects on VO2peak and the capillary supply to skeletal muscle. J Appl Physiol (Bethesda, Md: 1985). 1997;82(4):1305–10.
Snijders T, Nederveen JP, Bell KE, Lau SW, Mazara N, Kumbhare DA, et al. Prolonged exercise training improves the acute type II muscle fibre satellite cell response in healthy older men. J Physiol. 2019;597(1):105–19.
Moore DR, Kelly RP, Devries MC, Churchward-Venne TA, Phillips SM, Parise G, et al. Low-load resistance exercise during inactivity is associated with greater fibre area and satellite cell expression in older skeletal muscle. J Cachexia Sarcopenia Muscle. 2018;9(4):747–54.
Arentson-Lantz EJ, English KL, Paddon-Jones D, Fry CS. Fourteen days of bed rest induces a decline in satellite cell content and robust atrophy of skeletal muscle fibers in middle-aged adults. J Appl Physiol (Bethesda, Md: 1985). 2016;120(8):965–75.
Fujita S, Rasmussen BB, Cadenas JG, Drummond MJ, Glynn EL, Sattler FR, et al. Aerobic exercise overcomes the age-related insulin resistance of muscle protein metabolism by improving endothelial function and Akt/mammalian target of rapamycin signaling. Diabetes. 2007;56(6):1615–22.
Timmerman KL, Dhanani S, Glynn EL, Fry CS, Drummond MJ, Jennings K, et al. A moderate acute increase in physical activity enhances nutritive flow and the muscle protein anabolic response to mixed nutrient intake in older adults. Am J Clin Nutr. 2012;95(6):1403–12.
Phillips BE, Atherton PJ, Varadhan K, Limb MC, Wilkinson DJ, Sjøberg KA, et al. The effects of resistance exercise training on macro- and micro-circulatory responses to feeding and skeletal muscle protein anabolism in older men. J Physiol. 2015;593(12):2721–34.
Coggan AR, Spina RJ, Rogers MA, King DS, Brown M, Nemeth PM, et al. Histochemical and enzymatic characteristics of skeletal muscle in master athletes. J Appl Physiol (Bethesda, Md: 1985). 1990;68(5):1896–901.
Dickinson JM, Rasmussen BB. Amino acid transporters in the regulation of human skeletal muscle protein metabolism. Curr Opin Clin Nutr Metab Care. 2013;16(6):638–44.
Biolo G, Maggi SP, Williams BD, Tipton KD, Wolfe RR. Increased rates of muscle protein turnover and amino acid transport after resistance exercise in humans. Am J Physiol. 1995;268(3 Pt 1):E514–20.
Biolo G, Tipton KD, Klein S, Wolfe RR. An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein. Am J Physiol. 1997;273(1 Pt 1):E122–9.
Biolo G, Williams BD, Fleming RY, Wolfe RR. Insulin action on muscle protein kinetics and amino acid transport during recovery after resistance exercise. Diabetes. 1999;48(5):949–57.
Hundal HS, Taylor PM. Amino acid transceptors: gate keepers of nutrient exchange and regulators of nutrient signaling. Am J Physiol Endocrinol Metab. 2009;296(4):E603–13.
Dickinson JM, Drummond MJ, Coben JR, Volpi E, Rasmussen BB. Aging differentially affects human skeletal muscle amino acid transporter expression when essential amino acids are ingested after exercise. Clin Nutr (Edinburgh, Scotland). 2013;32(2):273–80.
Drummond MJ, Fry CS, Glynn EL, Timmerman KL, Dickinson JM, Walker DK, et al. Skeletal muscle amino acid transporter expression is increased in young and older adults following resistance exercise. J Appl Physiol (Bethesda, Md: 1985). 2011;111(1):135–42.
Agergaard J, Bülow J, Jensen JK, Reitelseder S, Bornø A, Drummond MJ, et al. Effect of light-load resistance exercise on postprandial amino acid transporter expression in elderly men. Physiol Rep. 2017;5(18):e13444.
Nicklin P, Bergman P, Zhang B, Triantafellow E, Wang H, Nyfeler B, et al. Bidirectional transport of amino acids regulates mTOR and autophagy. Cell. 2009;136(3):521–34.
Devries MC, McGlory C, Bolster DR, Kamil A, Rahn M, Harkness L, et al. Leucine, not total protein, content of a supplement is the primary determinant of muscle protein anabolic responses in healthy older women. J Nutr. 2018;148(7):1088–95.
Roberson PA, Mobley CB, Romero MA, Haun CT, Osburn SC, Mumford PW, et al. LAT1 protein content increases following 12 weeks of resistance exercise training in human skeletal muscle. Front Nutr. 2020;7:628405.
Drummond MJ, Dickinson JM, Fry CS, Walker DK, Gundermann DM, Reidy PT, et al. Bed rest impairs skeletal muscle amino acid transporter expression, mTORC1 signaling, and protein synthesis in response to essential amino acids in older adults. Am J Physiol Endocrinol Metab. 2012;302(9):E1113–22.
Kumar V, Selby A, Rankin D, Patel R, Atherton P, Hildebrandt W, et al. Age-related differences in the dose-response relationship of muscle protein synthesis to resistance exercise in young and old men. J Physiol. 2009;587(1):211–7.
Fry CS, Drummond MJ, Glynn EL, Dickinson JM, Gundermann DM, Timmerman KL, et al. Aging impairs contraction-induced human skeletal muscle mTORC1 signaling and protein synthesis. Skelet Muscle. 2011;1(1):11.
Stec MJ, Mayhew DL, Bamman MM. The effects of age and resistance loading on skeletal muscle ribosome biogenesis. J Appl Physiol (Bethesda, Md: 1985). 2015;119(8):851–7.
Burd NA, West DW, Moore DR, Atherton PJ, Staples AW, Prior T, et al. Enhanced amino acid sensitivity of myofibrillar protein synthesis persists for up to 24 h after resistance exercise in young men. J Nutr. 2011;141(4):568–73.
Hodson N, Philp A. The importance of mTOR trafficking for human skeletal muscle translational control. Exerc Sport Sci Rev. 2019;47(1):46–53.
Abou Sawan S, van Vliet S, Parel JT, Beals JW, Mazzulla M, West DWD, et al. Translocation and protein complex co-localization of mTOR is associated with postprandial myofibrillar protein synthesis at rest and after endurance exercise. Physiol Rep. 2018;6(5):e13628.
Abou Sawan S, van Vliet S, West DWD, Beals JW, Paluska SA, Burd NA, et al. Whole egg, but not egg white, ingestion induces mTOR colocalization with the lysosome after resistance exercise. Am J Physiol Cell Physiol. 2018;315(4):C537–43.
Song Z, Moore DR, Hodson N, Ward C, Dent JR, O’Leary MF, et al. Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle. Sci Rep. 2017;7(1):5028.
Moro T, Brightwell CR, Deer RR, Graber TG, Galvan E, Fry CS, et al. Muscle protein anabolic resistance to essential amino acids does not occur in healthy older adults before or after resistance exercise training. J Nutr. 2018;148(6):900–9.
Haran PH, Rivas DA, Fielding RA. Role and potential mechanisms of anabolic resistance in sarcopenia. J Cachexia Sarcopenia Muscle. 2012;3(3):157–62.
Breen L, Stokes KA, Churchward-Venne TA, Moore DR, Baker SK, Smith K, et al. Two weeks of reduced activity decreases leg lean mass and induces “anabolic resistance” of myofibrillar protein synthesis in healthy elderly. J Clin Endocrinol Metab. 2013;98(6):2604–12.
Smeuninx B, McKendry J, Wilson D, Martin U, Breen L. Age-related anabolic resistance of myofibrillar protein synthesis is exacerbated in obese inactive individuals. J Clin Endocrinol Metab. 2017;102(9):3535–45.
Greiwe JS, Cheng B, Rubin DC, Yarasheski KE, Semenkovich CF. Resistance exercise decreases skeletal muscle tumor necrosis factor alpha in frail elderly humans. FASEB J. 2001;15(2):475–82.
Lambert CP, Wright NR, Finck BN, Villareal DT. Exercise but not diet-induced weight loss decreases skeletal muscle inflammatory gene expression in frail obese elderly persons. J Appl Physiol (Bethesda, Md: 1985). 2008;105(2):473–8.
Aguiar SS, Sousa CV, Deus LA, Rosa TS, Sales MM, Neves RVP, et al. Oxidative stress, inflammatory cytokines and body composition of master athletes: the interplay. Exp Gerontol. 2020;130:110806.
Lavin KM, Perkins RK, Jemiolo B, Raue U, Trappe SW, Trappe TA. Effects of aging and lifelong aerobic exercise on basal and exercise-induced inflammation. J Appl Physiol (Bethesda, Md: 1985). 2020;128(1):87–99.
Lavin KM, Perkins RK, Jemiolo B, Raue U, Trappe SW, Trappe TA. Effects of aging and lifelong aerobic exercise on basal and exercise-induced inflammation in women. J Appl Physiol (Bethesda, Md: 1985). 2020;129(6):1493–504.
Costello JT, Bieuzen F, Bleakley CM. Where are all the female participants in Sports and Exercise Medicine research? Eur J Sport Sci. 2014;14(8):847–51.
Sjöström M, Oja P, Hagströmer M, Smith BJ, Bauman A. Health-enhancing physical activity across European Union countries: the Eurobarometer study. J Public Health. 2006;14(5):291–300.
van Schooten KS, van Dieen JH, Pijnappels M, Maier AB, van't Hul AJ, Niessen M, et al. The association between age and accelerometry-derived types of habitual daily activity: an observational study over the adult life span in the Netherlands. BMC Public Health. 2018;18(1):824.
Althoff T, Sosič R, Hicks JL, King AC, Delp SL, Leskovec J. Large-scale physical activity data reveal worldwide activity inequality. Nature. 2017;547(7663):336–9.
Holwerda AM, Paulussen KJM, Overkamp M, Goessens JPB, Kramer IF, Wodzig W, et al. Dose-dependent increases in whole-body net protein balance and dietary protein-derived amino acid incorporation into myofibrillar protein during recovery from resistance exercise in older men. J Nutr. 2019;149(2):221–30.
Moore DR. Maximizing post-exercise anabolism: the case for relative protein intakes. Front Nutr. 2019;6:147.
Devries MC, Breen L, Von Allmen M, MacDonald MJ, Moore DR, Offord EA, et al. Low-load resistance training during step-reduction attenuates declines in muscle mass and strength and enhances anabolic sensitivity in older men. Physiol Rep. 2015;3(8):e12493.
Aoyagi Y, Shephard RJ. Sex differences in relationships between habitual physical activity and health in the elderly: practical implications for epidemiologists based on pedometer/accelerometer data from the Nakanojo Study. Arch Gerontol Geriatr. 2013;56(2):327–38.
Moore DR. Keeping older muscle “young” through dietary protein and physical activity. Adv Nutr (Bethesda, Md). 2014;5(5):599s–607s.
Burd NA, Gorissen SH, van Loon LJ. Anabolic resistance of muscle protein synthesis with aging. Exerc Sport Sci Rev. 2013;41(3):169–73.
Phillips SM, Tipton KD, Aarsland A, Wolf SE, Wolfe RR. Mixed muscle protein synthesis and breakdown after resistance exercise in humans. Am J Physiol. 1997;273(1 Pt 1):E99-107.
Tipton KD, Borsheim E, Wolf SE, Sanford AP, Wolfe RR. Acute response of net muscle protein balance reflects 24-h balance after exercise and amino acid ingestion. Am J Physiol Endocrinol Metab. 2003;284(1):E76-89.
Rasmussen BB, Tipton KD, Miller SL, Wolf SE, Wolfe RR. An oral essential amino acid-carbohydrate supplement enhances muscle protein anabolism after resistance exercise. J Appl Physiol (Bethesda, Md: 1985). 2000;88(2):386–92.
West DW, Burd NA, Churchward-Venne TA, Camera DM, Mitchell CJ, Baker SK, et al. Sex-based comparisons of myofibrillar protein synthesis after resistance exercise in the fed state. J Appl Physiol (Bethesda, Md: 1985). 2012;112(11):1805–13.
Areta JL, Burke LM, Camera DM, West DW, Crawshay S, Moore DR, et al. Reduced resting skeletal muscle protein synthesis is rescued by resistance exercise and protein ingestion following short-term energy deficit. Am J Physiol Endocrinol Metab. 2014;306(8):E989–97.
Io M. Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein, and amino acids. Washington, DC: The National Academies Press; 2005.
Schoenfeld BJ, Aragon AA, Krieger JW. The effect of protein timing on muscle strength and hypertrophy: a meta-analysis. J Int Soc Sports Nutr. 2013;10(1):53.
Phillips SM, Van Loon LJ. Dietary protein for athletes: from requirements to optimum adaptation. J Sports Sci. 2011;29(Suppl 1):S29-38.
Burke LM, Slater G, Broad EM, Haukka J, Modulon S, Hopkins WG. Eating patterns and meal frequency of elite Australian athletes. Int J Sport Nutr Exerc Metab. 2003;13(4):521–38.
Areta JL, Burke LM, Ross ML, Camera DM, West DW, Broad EM, et al. Timing and distribution of protein ingestion during prolonged recovery from resistance exercise alters myofibrillar protein synthesis. J Physiol. 2013;591(9):2319–31.
Moore DR, Areta J, Coffey VG, Stellingwerff T, Phillips SM, Burke LM, et al. Daytime pattern of post-exercise protein intake affects whole-body protein turnover in resistance-trained males. Nutr Metab. 2012;9(1):91.
Yasuda J, Tomita T, Arimitsu T, Fujita S. Evenly distributed protein intake over 3 meals augments resistance exercise-induced muscle hypertrophy in healthy young men. J Nutr. 2020;150(7):1845–51.
Stokes T, Hector AJ, Morton RW, McGlory C, Phillips SM. Recent perspectives regarding the role of dietary protein for the promotion of muscle hypertrophy with resistance exercise training. Nutrients. 2018;10(2):180.
Loenneke JP, Loprinzi PD, Murphy CH, Phillips SM. Per meal dose and frequency of protein consumption is associated with lean mass and muscle performance. Clin Nutr (Edinburgh, Scotland). 2016;35(6):1506–11.
Loprinzi PD, Loenneke JP, Hamilton DL. Leisure time sedentary behavior, physical activity and frequency of protein consumption on lower extremity strength and lean mass. Eur J Clin Nutr. 2017;71(12):1399–404.
Hudson JL, Iii REB, Campbell WW. Protein distribution and muscle-related outcomes: does the evidence support the concept? Nutrients. 2020;12(5):1441.
Jespersen SE, Agergaard J. Evenness of dietary protein distribution is associated with higher muscle mass but not muscle strength or protein turnover in healthy adults: a systematic review. Eur J Nutr. 2021. https://doi.org/10.1007/s00394-021-02487-2.
Mazzulla M, Sawan SA, Williamson E, Hannaian SJ, Volterman KA, West DWD, et al. Protein intake to maximize whole-body anabolism during postexercise recovery in resistance-trained men with high habitual intakes is severalfold greater than the current recommended dietary allowance. J Nutr. 2020;150(3):505–11.
Malowany JM, West DWD, Williamson E, Volterman KA, Abou Sawan S, Mazzulla M, et al. Protein to maximize whole-body anabolism in resistance-trained females after exercise. Med Sci Sports Exerc. 2019;51(4):798–804.
Morton RW, Murphy KT, McKellar SR, Schoenfeld BJ, Henselmans M, Helms E, et al. A systematic review, meta-analysis and meta-regression of the effect of protein supplementation on resistance training-induced gains in muscle mass and strength in healthy adults. Br J Sports Med. 2018;52(6):376–84.
Di Girolamo FG, Situlin R, Fiotti N, Tence M, De Colle P, Mearelli F, et al. Higher protein intake is associated with improved muscle strength in elite senior athletes. Nutrition (Burbank, Los Angeles County, Calif). 2017;42:82–6.
Kerksick CM, Arent S, Schoenfeld BJ, Stout JR, Campbell B, Wilborn CD, et al. International society of sports nutrition position stand: nutrient timing. J Int Soc Sports Nutr. 2017;14:33.
Gillen JB, Trommelen J, Wardenaar FC, Brinkmans NY, Versteegen JJ, Jonvik KL, et al. Dietary protein intake and distribution patterns of well-trained dutch athletes. Int J Sport Nutr Exerc Metab. 2017;27(2):105–14.
Kato H, Suzuki K, Bannai M, Moore DR. Branched-chain amino acids are the primary limiting amino acids in the diets of endurance-trained men after a bout of prolonged exercise. J Nutr. 2018;148(6):925–31.
Meredith CN, Zackin MJ, Frontera WR, Evans WJ. Dietary protein requirements and body protein metabolism in endurance-trained men. J Appl Physiol (Bethesda, Md: 1985). 1989;66(6):2850–6.
Kato H, Suzuki K, Bannai M, Moore DR. Protein requirements are elevated in endurance athletes after exercise as determined by the indicator amino acid oxidation method. PLoS ONE. 2016;11(6):e0157406.
Humayun MA, Elango R, Ball RO, Pencharz PB. Reevaluation of the protein requirement in young men with the indicator amino acid oxidation technique. Am J Clin Nutr. 2007;86(4):995–1002.
Phillips SM, Moore DR, Tang JE. A critical examination of dietary protein requirements, benefits, and excesses in athletes. Int J Sport Nutr Exerc Metab. 2007;17(Suppl):S58-76.
Tipton KD, Witard OC. Protein requirements and recommendations for athletes: relevance of ivory tower arguments for practical recommendations. Clin Sports Med. 2007;26(1):17–36.
Williamson E, Kato H, Volterman KA, Suzuki K, Moore DR. The effect of dietary protein on protein metabolism and performance in endurance-trained males. Med Sci Sports Exerc. 2019;51(2):352–60.
Beshgetoor D, Nichols JF. Dietary intake and supplement use in female master cyclists and runners. Int J Sport Nutr Exerc Metab. 2003;13(2):166–72.
Churchward-Venne TA, Pinckaers PJM, Smeets JSJ, Betz MW, Senden JM, Goessens JPB, et al. Dose-response effects of dietary protein on muscle protein synthesis during recovery from endurance exercise in young men: a double-blind randomized trial. Am J Clin Nutr. 2020;112(2):303–17.
Mazzulla M, Volterman KA, Packer JE, Wooding DJ, Brooks JC, Kato H, et al. Whole-body net protein balance plateaus in response to increasing protein intakes during post-exercise recovery in adults and adolescents. Nutr Metab. 2018;15:62.
Moore DR. One size doesn’t fit all: postexercise protein requirements for the endurance athlete. Am J Clin Nutr. 2020;112(2):249–50.
Howarth KR, Phillips SM, MacDonald MJ, Richards D, Moreau NA, Gibala MJ. Effect of glycogen availability on human skeletal muscle protein turnover during exercise and recovery. J Appl Physiol (Bethesda, Md: 1985). 2010;109(2):431–8.
Hamadeh MJ, Devries MC, Tarnopolsky MA. Estrogen supplementation reduces whole body leucine and carbohydrate oxidation and increases lipid oxidation in men during endurance exercise. J Clin Endocrinol Metab. 2005;90(6):3592–9.
Phillips SM, Atkinson SA, Tarnopolsky MA, MacDougall JD. Gender differences in leucine kinetics and nitrogen balance in endurance athletes. J Appl Physiol (Bethesda, Md: 1985). 1993;75(5):2134–41.
Manetta J, Brun JF, Prefaut C, Mercier J. Substrate oxidation during exercise at moderate and hard intensity in middle-aged and young athletes vs sedentary men. Metab Clin Exp. 2005;54(11):1411–9.
Dubé JJ, Broskey NT, Despines AA, Stefanovic-Racic M, Toledo FG, Goodpaster BH, et al. Muscle characteristics and substrate energetics in lifelong endurance athletes. Med Sci Sports Exerc. 2016;48(3):472–80.
Doering TM, Reaburn PR, Phillips SM, Jenkins DG. Postexercise dietary protein strategies to maximize skeletal muscle repair and remodeling in masters endurance athletes: a review. Int J Sport Nutr Exerc Metab. 2016;26(2):168–78.
Doering TM, Jenkins DG, Reaburn PR, Borges NR, Hohmann E, Phillips SM. Lower integrated muscle protein synthesis in masters compared with younger athletes. Med Sci Sports Exerc. 2016;48(8):1613–8.
Doering TM, Reaburn PR, Borges NR, Cox GR, Jenkins DG. The effect of higher than recommended protein feedings post-exercise on recovery following downhill running in masters triathletes. Int J Sport Nutr Exerc Metab. 2017;27(1):76–82.
Waskiw-Ford M, Hannaian S, Duncan J, Kato H, Abou Sawan S, Locke M, et al. Leucine-enriched essential amino acids improve recovery from post-exercise muscle damage independent of increases in integrated myofibrillar protein synthesis in young men. Nutrients. 202011;12(4):1061.
Pavis GF, Jameson TSO, Dirks ML, Lee BP, Abdelrahman DR, Murton AJ, et al. Improved recovery from skeletal muscle damage is largely unexplained by myofibrillar protein synthesis or inflammatory and regenerative gene expression pathways. Am J Physiol Endocrinol Metab. 2021;320(2):E291-e305.
Davies RW, Carson BP, Jakeman PM. The effect of whey protein supplementation on the temporal recovery of muscle function following resistance training: a systematic review and meta-analysis. Nutrients. 2018;10(2):221.
Snijders T, Trommelen J, Kouw IWK, Holwerda AM, Verdijk LB, van Loon LJC. The impact of pre-sleep protein ingestion on the skeletal muscle adaptive response to exercise in humans: an update. Front Nutr. 2019;6:17.
Res PT, Groen B, Pennings B, Beelen M, Wallis GA, Gijsen AP, et al. Protein ingestion before sleep improves postexercise overnight recovery. Med Sci Sports Exerc. 2012;44(8):1560–9.
Trommelen J, Kouw IWK, Holwerda AM, Snijders T, Halson SL, Rollo I, et al. Presleep dietary protein-derived amino acids are incorporated in myofibrillar protein during postexercise overnight recovery. Am J Physiol Endocrinol Metab. 2018;314(5):E457–67.
Holwerda AM, Kouw IW, Trommelen J, Halson SL, Wodzig WK, Verdijk LB, et al. Physical activity performed in the evening increases the overnight muscle protein synthetic response to presleep protein ingestion in older men. J Nutr. 2016;146(7):1307–14.
Snijders T, Res PT, Smeets JS, van Vliet S, van Kranenburg J, Maase K, et al. Protein ingestion before sleep increases muscle mass and strength gains during prolonged resistance-type exercise training in healthy young men. J Nutr. 2015;145(6):1178–84.
Holwerda AM, Overkamp M, Paulussen KJM, Smeets JSJ, van Kranenburg J, Backx EMP, et al. Protein supplementation after exercise and before sleep does not further augment muscle mass and strength gains during resistance exercise training in active older men. J Nutr. 2018;148(11):1723–32.
West DWD, Abou Sawan S, Mazzulla M, Williamson E, Moore DR. Whey protein supplementation enhances whole body protein metabolism and performance recovery after resistance exercise: a double-blind crossover study. Nutrients. 2017;9(7):735.
Hannaian SJ, Orlando MN, Abou Sawan S, Mazzulla M, West DWD, Moore DR. Protein timing does not affect next-day recovery of strength or power but may enhance aerobic adaptations to short-term variable intensity exercise training in recreationally active males: a pilot study. Front Sports Active Liv. 2020;2:568740.
Impey SG, Hearris MA, Hammond KM, Bartlett JD, Louis J, Close GL, et al. Fuel for the work required: a theoretical framework for carbohydrate periodization and the glycogen threshold hypothesis. Sports Med (Auckland, NZ). 2018;48(5):1031–48.
Stellingwerf T. Case study: nutrition and training periodization in three elite marathon runners. Int J Sport Nutr Exerc Metab. 2012;22(5):392–400.
Gillen JB, West DWD, Williamson EP, Fung HJW, Moore DR. Low-carbohydrate training increases protein requirements of endurance athletes. Med Sci Sports Exerc. 2019;51(11):2294–301.
Tinsley GM, La Bounty PM. Effects of intermittent fasting on body composition and clinical health markers in humans. Nutr Rev. 2015;73(10):661–74.
Marquet LA, Brisswalter J, Louis J, Tiollier E, Burke LM, Hawley JA, et al. Enhanced endurance performance by periodization of carbohydrate intake: “sleep low” strategy. Med Sci Sports Exerc. 2016;48(4):663–72.
Williamson E, Moore DR. A Muscle-centric perspective on intermittent fasting: a suboptimal dietary strategy for supporting muscle protein remodeling and muscle mass? Front Nutr. 2021;8(319):640621.
Stratton MT, Tinsley GM, Alesi MG, Hester GM, Olmos AA, Serafini PR, et al. Four weeks of time-restricted feeding combined with resistance training does not differentially influence measures of body composition, muscle performance, resting energy expenditure, and blood biomarkers. Nutrients. 2020;12(4):1126.
Tinsley GM, Forsse JS, Butler NK, Paoli A, Bane AA, La Bounty PM, et al. Time-restricted feeding in young men performing resistance training: a randomized controlled trial. Eur J Sport Sci. 2017;17(2):200–7.
Wall BT, Burd NA, Franssen R, Gorissen SH, Snijders T, Senden JM, et al. Presleep protein ingestion does not compromise the muscle protein synthetic response to protein ingested the following morning. Am J Physiol Endocrinol Metab. 2016;311(6):E964–73.
Brady AJ, Langton HM, Mulligan M, Egan B. Effects of eight weeks of 16:8 time-restricted eating in male middle- and long-distance runners. Med Sci Sports Exerc. 2020;53(3):633–42.
Moro T, Tinsley G, Longo G, Grigoletto D, Bianco A, Ferraris C, et al. Time-restricted eating effects on performance, immune function, and body composition in elite cyclists: a randomized controlled trial. J Int Soc Sports Nutr. 2020;17(1):65.
Chambers TL, Burnett TR, Raue U, Lee GA, Finch WH, Graham BM, et al. Skeletal muscle size, function, and adiposity with lifelong aerobic exercise. J Appl Physiol (Bethesda, Md: 1985). 2020;128(2):368–78.
Knapik JJ, Steelman RA, Hoedebecke SS, Austin KG, Farina EK, Lieberman HR. Prevalence of dietary supplement use by athletes: systematic review and meta-analysis. Sports Med (Auckland, NZ). 2016;46(1):103–23.
Gilani GS, Cockell KA, Sepehr E. Effects of antinutritional factors on protein digestibility and amino acid availability in foods. J AOAC Int. 2005;88(3):967–87.
Burd NA, Beals JW, Martinez IG, Salvador AF, Skinner SK. Food-first approach to enhance the regulation of post-exercise skeletal muscle protein synthesis and remodeling. Sports Med (Auckland, NZ). 2019;49(Suppl 1):59–68.
Vliet SV, Beals JW, Martinez IG, Skinner SK, Burd NA. Achieving optimal post-exercise muscle protein remodeling in physically active adults through whole food consumption. Nutrients. 2018;10(2):224.
van Vliet S, Shy EL, Abou Sawan S, Beals JW, West DW, Skinner SK, et al. Consumption of whole eggs promotes greater stimulation of postexercise muscle protein synthesis than consumption of isonitrogenous amounts of egg whites in young men. Am J Clin Nutr. 2017;106(6):1401–12.
Monteyne AJ, Coelho MOC, Porter C, Abdelrahman DR, Jameson TSO, Jackman SR, et al. Mycoprotein ingestion stimulates protein synthesis rates to a greater extent than milk protein in rested and exercised skeletal muscle of healthy young men: a randomized controlled trial. Am J Clin Nutr. 2020;112(2):318–33.
van Vliet S, Burd NA, van Loon LJ. The skeletal muscle anabolic response to plant-versus animal-based protein consumption. J Nutr. 2015;145(9):1981–91.
McKean KA, Manson NA, Stanish WD. Musculoskeletal injury in the masters runners. Clin J Sport Med. 2006;16(2):149–54.
Shaw G, Lee-Barthel A, Ross ML, Wang B, Baar K. Vitamin C-enriched gelatin supplementation before intermittent activity augments collagen synthesis. Am J Clin Nutr. 2017;105(1):136–43.
Lis DM, Baar K. Effects of different vitamin C-enriched collagen derivatives on collagen synthesis. Int J Sport Nutr Exerc Metab. 2019;29(5):526–31.
Close GL, Sale C, Baar K, Bermon S. Nutrition for the prevention and treatment of injuries in track and field athletes. Int J Sport Nutr Exerc Metab. 2019;29(2):189–97.
Paul C, Leser S, Oesser S. Significant amounts of functional collagen peptides can be incorporated in the diet while maintaining indispensable amino acid balance. Nutrients. 2019;11(5):1079.
Oikawa SY, MacInnis MJ, Tripp TR, McGlory C, Baker SK, Phillips SM. Lactalbumin, Not collagen, augments muscle protein synthesis with aerobic exercise. Med Sci Sports Exerc. 2020;52(6):1394–403.
Oikawa SY, Kamal MJ, Webb EK, McGlory C, Baker SK, Phillips SM. Whey protein but not collagen peptides stimulate acute and longer-term muscle protein synthesis with and without resistance exercise in healthy older women: a randomized controlled trial. Am J Clin Nutr. 2020;111(3):708–18.
Brook MS, Scaife P, Bass JJ, Cegielski J, Watanabe S, Wilkinson DJ, et al. A collagen hydrolysate/milk protein-blend stimulates muscle anabolism equivalently to an isoenergetic milk protein-blend containing a greater quantity of essential amino acids in older men. Clin Nutr. 2021;40(6):4456–64.
Meléndez-Hevia E, De Paz-Lugo P, Cornish-Bowden A, Cárdenas ML. A weak link in metabolism: the metabolic capacity for glycine biosynthesis does not satisfy the need for collagen synthesis. J Biosci. 2009;34(6):853–72.
Lepers R, Cattagni T. Do older athletes reach limits in their performance during marathon running? Age (Dordr). 2012;34(3):773–81.
Miller BF, Hansen M, Olesen JL, Flyvbjerg A, Schwarz P, Babraj JA, et al. No effect of menstrual cycle on myofibrillar and connective tissue protein synthesis in contracting skeletal muscle. Am J Physiol Endocrinol Metab. 2006;290(1):E163–8.
Mercer D, Convit L, Condo D, Carr AJ, Hamilton DL, Slater G, et al. Protein requirements of pre-menopausal female athletes: systematic literature review. Nutrients. 2020;12(11):3527.
Javed AA, Mayhew AJ, Shea AK, Raina P. Association between hormone therapy and muscle mass in postmenopausal women: a systematic review and meta-analysis. JAMA Netw Open. 2019;2(8):e1910154.
Brown M, Birge SJ, Kohrt WM. Hormone replacement therapy does not augment gains in muscle strength or fat-free mass in response to weight-bearing exercise. J Gerontol A Biol Sci Med Sci. 1997;52(3):B166–70.
Figueroa A, Going SB, Milliken LA, Blew RM, Sharp S, Teixeira PJ, et al. Effects of exercise training and hormone replacement therapy on lean and fat mass in postmenopausal women. J Gerontol A Biol Sci Med Sci. 2003;58(3):266–70.
Smith GI, Mittendorfer B. Sexual dimorphism in skeletal muscle protein turnover. J Appl Physiol (Bethesda, Md: 1985). 2016;120(6):674–82.
Smith GI, Reeds DN, Hall AM, Chambers KT, Finck BN, Mittendorfer B. Sexually dimorphic effect of aging on skeletal muscle protein synthesis. Biol Sex Differ. 2012;3(1):11.
Smith GI, Atherton P, Villareal DT, Frimel TN, Rankin D, Rennie MJ, et al. Differences in muscle protein synthesis and anabolic signaling in the postabsorptive state and in response to food in 65–80 year old men and women. PLoS ONE. 2008;3(3):e1875.
Piasecki J, McPhee JS, Hannam K, Deere KC, Elhakeem A, Piasecki M, et al. Hip and spine bone mineral density are greater in master sprinters, but not endurance runners compared with non-athletic controls. Arch Osteoporos. 2018;13(1):72.
Groenendijk I, den Boeft L, van Loon LJC, de Groot L. High versus low dietary protein intake and bone health in older adults: a systematic review and meta-analysis. Comput Struct Biotechnol J. 2019;17:1101–12.
Dawson-Hughes B, Harris SS. Calcium intake influences the association of protein intake with rates of bone loss in elderly men and women. Am J Clin Nutr. 2002;75(4):773–9.
Frassetto L, Morris RC Jr, Sellmeyer DE, Todd K, Sebastian A. Diet, evolution and aging—the pathophysiologic effects of the post-agricultural inversion of the potassium-to-sodium and base-to-chloride ratios in the human diet. Eur J Nutr. 2001;40(5):200–13.
Sale C, Elliott-Sale KJ. Nutrition and athlete bone health. Sports Med (Auckland, NZ). 2019;49(Suppl 2):139–51.
Stiefel M, Knechtle B, Lepers R. Master triathletes have not reached limits in their Ironman triathlon performance. Scand J Med Sci Sports. 2014;24(1):89–97.
Doering TM, Reaburn PR, Cox G, Jenkins DG. Comparison of postexercise nutrition knowledge and postexercise carbohydrate and protein intake between australian masters and younger triathletes. Int J Sport Nutr Exerc Metab. 2016;26(4):338–46.
Subar AF, Freedman LS, Tooze JA, Kirkpatrick SI, Boushey C, Neuhouser ML, et al. Addressing current criticism regarding the value of self-report dietary data. J Nutr. 2015;145(12):2639–45.