FormalPara Key Summary Points
Why carry out this study?
Both pain and cognitive impairment are common symptoms that often coexist, especially in older individuals.
Although these two symptoms interact with each other, the neural mechanisms bridging them remain unclear.
The present study used the functional neuroimaging technique of magnetoencephalography to gain insight into the neural mechanisms.
What was learned from the study?
Resting-state brain activity recorded before and after the surgical treatment of severe pain correlated with subjective levels of pain and cognitive status when participants suffered from severe pain, but not after the pain was relieved.
The confirmed interaction between pain and cognitive function underscores the importance of treating pain to prevent patients from developing cognitive impairment.

Digital Features

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Pain, like fever or fatigue, is an important bio-alarm system that can be used to ensure the health and safety of the individual who experiences it [1, 2]. It is a common, subjective, unpleasant sensory and emotional experience associated with actual or potential tissue damage. The lifetime incidence of radicular pain and low back pain ranges between 13 and 40% and between 50 and 80%, respectively, with a higher prevalence in older populations [3,4,5,6,7]. Notably, pain is one of the major reasons for visits to medical facilities [8, 9].

As a multi-systemic phenomenon, pain relies on a widely distributed cortical/subcortical network known as the 'pain matrix' [1, 10]: a network that affects cognitive functions such as attention, memory, and executive function [11,12,13,14,15,16,17,18]. This link is evidenced by psychomotor slowing in patients with diabetic peripheral neuropathy [19], the association between the subjective level of pain and poor performance on executive functioning tasks in patients with rheumatoid arthritis [20], and the identification of pain as a risk factor for dementia [17]. On the other hand, cognitive status mediates the subjective level of pain. Patients with dementia exhibit larger responses to experimental nociceptive stimulation [21,22,23]. In clinical situations, patients with cognitive decline often complain of pain in response to low-level stimulation, such as the removal of surgical tape from their skin. However, while the interaction between pain and cognitive function is clear, the neural mechanisms underlying their relationship remain unclear [16].

Magnetoencephalography (MEG) is a non-invasive neuroimaging technique that is often used to record and evaluate brain activities in studies of pain and cognitive dysfunction [24,25,26,27,28,29,30]. Its widespread use is partly attributed to its ease, practicality, and safety of implementation; it requires only a few minutes to prepare, can complete a scan within 5 min, and is easy to use repeatedly [31]. The present study compared the resting-state brain activity (spontaneous neural oscillations), subjective levels of pain, and cognitive status of patients before and after undergoing selective nerve root blocks (SNRB) to determine the relationship between pain and cognitive dysfunction at the neural level.


Participants and Procedures

Thirty-eight patients with low back pain were enrolled in this study (17 women; mean age, 68.9 ± 10.3 years; age range, 47–84 years). They visited the outpatient Department of Orthopaedics at Kumagaya General Hospital, where they were diagnosed with sciatic pain by orthopaedic surgeons and were scheduled to receive SNRB for pain management. Patients reported pain on the left side (n = 22), right side (n = 11), both sides (n = 4), and midline (n = 1). This study was partly conducted during the period in which patients waited to receive surgery during ordinal clinical practice. Participants saw an orthopaedic surgeon who checked for any contraindications for receiving SNRB, and were provided explanations about the study and completed informed consent forms to indicate their agreement to participate. The first MEG scans and subjective pain assessments were performed in the MEG room (before SNRB). The patients then received SNRB in the operating room. After the SNRB, the patients completed the second MEG scans and subjective assessments. Finally, each patient’s cognitive status was assessed by clinical psychologists.

Subjective and Cognitive Assessments

The subjective level of pain of the participants was assessed using a visual analogue scale (VAS) before and after SNRB (pain-VAS). Pain-VAS scores range from 0 to 10, where higher values indicated more severe pain. Cognitive status was assessed using the Japanese version of the Mini-Mental State Examination (MMSE-J) after the patients underwent SNRB [32, 33]. The MMSE-J score ranges from 0 to 30, where lower MMSE-J scores indicated more severe cognitive impairment. A cut-off score of 23/24 was used to indicate dementia [34]. We did not assess cognitive status before SNRB, in order to avoid the ‘practice effect’ (see Limitations). All procedures were completed within a few hours.

MEG Scanning

Spontaneous neural oscillations were recorded for 5 min using a 160-channel whole-head type magnetoencephalography system (RICOH160-1: RICOH, Tokyo, Japan) before and after SNRB on the same day. During the scan, participants were placed in a magnetically shielded room and were asked to remain calm and to remain supine with their eyes closed. The scanning conditions were controlled for consistency, and the participants were made as comfortable as possible. The sensor and reference coils were gradiometers 15.5 mm in diameter and 50 mm at baseline, and each pair of sensor coils was separated by a distance of 23 mm. The sampling frequency was 2000 Hz. During the recording process, 500 Hz low-pass filtering was used. To co-register, the MEG source images with standard magnetic resonance imaging (MRI) structural brain images, and three fiducial magnetic marker coils were placed on each participant's face (5 mm above the nasion and bilaterally 10 mm in front of the tragus) during the MEG scan.

MEG Analysis

MEG data were preprocessed offline using the Statistical Parametric Mapping (SPM)-12 software package (Wellcome Trust Centre for Neuroimaging, London, UK; and the MEEG Automated Workflow (MEAW) system ( The analytical procedure was performed as described previously [31]. Continuous MEG signals were divided into 10-s segments. Since a utility frequency was generated during the experimental environment, a 50-Hz band-stop filter was applied to the epoched data. The filtered data were used directly for source-level analyses. To identify the specific locations of the brain regions responsible for producing the resting-state-induced components, source inversion procedures were applied separately to the oscillation components of delta (0–3 Hz), theta (4–7 Hz), alpha (8–12 Hz), beta (13–25 Hz), and gamma (low gamma, 26–40 Hz; high gamma, 41–80 Hz). We used a maximal smoothness algorithm with a spatially coherent source model (i.e., COH algorithms implemented in SPM-12) [35], which is comparable to standardised low-resolution brain electromagnetic tomography (sLORETA) [36]. The COH algorithm is a popular source inversion algorithm that is often used in clinical environments [37, 38]. Using a single-shell model with canonical MR images provided by SPM-12, forward modelling was performed for the whole brain. The source inversion and estimation were performed by applying filters corresponding to each frequency band (from delta to high gamma). Source priors were not used for source estimation. The estimated oscillatory intensity at each location was saved as a 'source image' digital file in the Neuroimaging Informatics Technology Initiative (NIfTI) format. The source images were smoothed (20 × 20 × 20 mm) and used in the second (group)-level analysis. Two types of second (group)-level analyses were conducted (1) to determine whether the intensities correlated with subjective levels of pain (pain-VAS) readings or cognitive status (MMSE-J score; blue arrows in Fig. 1), and (2) to find brain regions in which the intensities of the regional spontaneous neural oscillation differed across the SNRB (red arrow in Fig. 1). In the first analysis, the source images were separately regressed by the pain-VAS readings (before and after the SNRB) and MMSE-J scores. Both the positive and negative effects of the predictors were evaluated by building t-contrasts of +1 and −1. In the second analysis, source images were compared for each patient using the paired t test at each frequency band across SNRB. In this study, we report the source locations of peak level activations at a significance threshold of P = 0.05 (corrected for family-wise error (FWE) rate) and a cluster extent at k > 10 (80 mm3) [39]. Cortical regions where the peaks of the estimated sources were located were identified with SPM-12.

Fig. 1
figure 1

Analytical procedure. The red arrow indicates analysis conducted to find brain regions where oscillatory intensities differed across SNRB. The blue arrows indicate analyses performed to find brain regions where oscillatory intensities correlated with the results obtained from subjective and cognitive assessments. The black arrows represent the comparison between the results collected from the subjective and cognitive assessments. Broken arrows represent MEG preprocessing. The participants' ages (a predictor used in the analysis procedure) is not described in this figure

Statistical Analysis

To evaluate the effects of the SNRB on the subjective experience of pain, we compared the pain-VAS readings across SNRB using a non-parametric bootstrapping approach. The changes in the score across SNRB were evaluated. The difference was resampled (with replacement) 20,000 times across all patients, and the percentage of the resampled differences, being larger or smaller than 0 (the smaller value), was taken as the significance level.

To evaluate the directional relationship between the subjective level of pain and cognitive status, we used a curve-fitting approach combined with non-parametric bootstrapping statistics. Pairs of predictor and response variables were prepared (Table 1). For each pair, predictor and response variables were resampled (with replacement) 20,000 times across all patients. In each iteration of the resampling, the response variable was fitted into one-dimensional (linear) and two-dimensional (quadratic) curves using a least-squares algorithm, and estimated coefficients were stored. Finally, the percentage of the resampled coefficients, being larger or smaller than 0 (the smaller value), was calculated for the linear and quadratic coefficients, respectively, which were then used as the significance level (P value). We report the average coefficients across the resampled data and the corresponding P values.

Table 1 Behavioural data of interest and results of polynomial fitting analysis

Compliance with Ethics Guidelines

This study was conducted in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of Kumagaya General Hospital (approval number 19). The details of the study (i.e., the objectives and procedure) were explained by the orthopaedic surgeon (co-author S.K.). He also explained that participants could decline their participation at any time without stating their reasons, and that this would not lead to any disadvantage in terms of their clinical treatment. All participants gave written informed consent to participate in this study.


Subjective and Cognitive Assessments

The mean pain-VAS (± SD) readings were 7.32 ± 2.02 before SNRB and 2.39 ± 2.51 after SNRB. The change across SNRB was statistically significant (P < 0.001). Polynomial curve-fitting analysis revealed that the MMSE-J score was predicted by the change in pain-VAS readings (Table 1). Both quadratic (average: 0.07, P = 0.015) and linear (average: 0.50, P = 0.042) coefficients were statistically larger than zero. No other directional relationships were found between the four factors: pain-VAS readings before and after SNRB, change in pain-VAS readings, and MMSE-J score.

Table 2 Correlation between resting-state oscillatory activity with subjective level of pain and cognitive status (corresponding to Fig. 1)

Regional Spontaneous Neural Oscillations

Before SNRB, while the patients experienced severe pain, the delta intensity in the right precentral gyrus, right middle temporal gyrus, and left superior frontal gyrus correlated negatively with the pain-VAS readings (Fig. 2, Table 2). High gamma intensity in the right anterior insula cortex and right middle temporal gyrus negatively correlated with the MMSE-J score. These regions overlapped around the right precentral gyrus. After SNRB, when the severe pain diminished, no significant correlation was found between the intensity of regional spontaneous neural oscillations at any frequency and the pain-VAS readings or MMSE-J scores. No significant changes in the intensity of the regional spontaneous neural oscillations were found at any frequency across SNRB.

Fig. 2
figure 2

Brain regions with oscillation intensities that correlated with subjective levels of pain and cognitive status. Blue colour indicates regions in which delta intensity was negatively correlated with the subjective level of pain (Pain-VAS). Red colour represents the region in which high gamma intensity was negatively correlated with cognitive status (MMSE-J score). The 3D image was created using MRIcroGL


The present study showed that regional spontaneous neural oscillations (i.e., resting-state brain activity) were correlated with subjective levels of pain (pain-VAS) and cognitive status (MMSE-J score) in participants suffering from severe pain (before the SNRB), but not after the severe pain was relieved (after the SNRB). More specifically, this study found that (1) the subjective level of pain correlated with regional spontaneous neural oscillations in the right temporal lobe at a low frequency when the pain was severe (before SNRB), (2) cognitive status was related to regional spontaneous neural oscillations in the right hemisphere at a high frequency when the pain was severe (before SNRB), and (3) these relationships disappeared after the severe pain was relieved (after SNRB).

Pain is an unpleasant, subjective experience from which approximately 40% of the general population suffers in one form or another [40, 41]. Pain and cognitive dysfunction, including dementia, are particularly common among older individuals [3,4,5,6]; a new patient is diagnosed with dementia every 7 seconds [42]. These two conditions (pain and cognitive dysfunction) are demonstrably interrelated [11,12,13,14, 43], and pain has been identified as a risk factor for dementia [17]. However, the neural mechanisms underlying their interaction remain unclear.

The present study found that SNRB successfully reduced the subjective level of pain of the enrolled patients. The shift in the subjective levels of pain allowed us to investigate the correlation between pain and cognitive status. Subjective and cognitive assessments revealed a part of this bridge: SNRB was more effective [predictor] among patients (i.e., patients who experienced larger degrees of pain relief following SNRB) with higher MMSE-J scores after SNRB (i.e., better cognitive function) [response].

The oscillatory intensity analysis visualised regions whose activity correlated with subjective levels of pain and cognitive status under severe pain (before SNRB); these regions were found to overlap. Increased subjective levels of pain corresponded to the attenuated intensity of low-frequency oscillatory (delta) intensity in the right precentral gyrus, right middle temporal gyrus, and left superior frontal gyrus when the pain was very severe (i.e., before the SNRB; blue regions in Fig. 2). We ascribe the associations between oscillatory changes and the low frequency to the production of low-frequency spontaneous neural oscillations by the distributed networks, such as the pain system. We attribute the associations between oscillatory changes and the high frequency to the production of high-frequency spontaneous neural oscillations by local activity [44,45,46,47,48,49,50,51]. Studies have demonstrated that regional spontaneous neural oscillation at low frequency (3–8 Hz) is enhanced by chronic pain [52, 53]. Previous investigations have revealed that the 'pain matrix' consists of the primary and secondary somatosensory, insular, anterior cingulate, and prefrontal cortices and the thalamus [54]. The precentral gyrus is known as a primary motor area and contributes to the pain processing and chorionic pain reorganisation in the motor cortex; it is not, however, considered a component of the 'pain matrix' [55, 56]. Hence, experiencing pain can affect regional spontaneous neural oscillations in the motor cortex. The temporal gyrus also contributes to pain processing [57, 58]. A previous study showed that painful stimulation evoked changes in local field potentials at low frequencies (theta) in the right medial temporal gyrus in humans [57]. Changes in low-frequency oscillations in the precentral and temporal gyri were also found in the right hemisphere. These findings are consistent with the observations that pain perception is dominated by the right hemisphere and that people are more sensitive to painful stimulation on the left side of the body [59,60,61]. Meanwhile, the left superior frontal gyrus also showed attenuated low-frequency oscillation accompanied by increasing levels of subjective pain. This finding could represent different changes in the precentral and middle temporal gyri because it is situated in the left hemisphere. The left superior frontal gyrus is known as a key component of the neural network of working memory [62, 63], although it contributes to pain processing as well [61]. Working memory deteriorates in the early stages of dementia [64], a trend that is consistent with the correlation between rostral low-frequency oscillatory (delta) intensity and cognitive function [65, 66]. Therefore, we speculate that attenuated low-frequency oscillatory (delta) intensity represents a part of the neural mechanism that bridges pain perception and cognitive dysfunction. Pain-related attenuation in the low-frequency oscillation (delta) affected cognitive regions, including the left superior frontal gyrus, across the 'pain matrix'.

The oscillatory intensity analysis also visualised brain regions whose activity was related to the patients' cognitive status under severe pain (i.e., before SNRB; red regions in Fig. 2). The MMSE-J score was negatively correlated with high-frequency oscillatory (gamma) intensity in the right insular cortex and right middle temporal gyrus, both of which contribute to pain perception. The right anterior insula is functionally connected with frontal regions and is considered to be a major component of the 'pain matrix' and the attention system [15, 54]. The supposition that the right anterior insula modulates pain processing is supported by the observation that the experience of pain is affected by attention [67]. Atrophy of the right anterior insula in patients with Alzheimer’s disease is related to their experience of hallucinations, which is a major symptom of dementia [68, 69]. Patients who experience hallucination achieve lower scores on the MMSE [69]. The previous observations agree with the present findings that high-frequency oscillatory intensity in the right anterior insula correlates with scores on the MMSE. The middle temporal gyrus also contributes to pain processing [57, 58], plays important roles in cognitive functions, and is affected by dementia [70,71,72]. A previous study showed that MMSE scores correlated with the volume of the right temporal gyrus [73]. It is reasonable that changes in oscillatory intensity in the right anterior insular cortex and the right middle temporal gyrus correlate with cognitive status, as assessed using the MMSE-J. However, it is unclear why cognitive dysfunction was associated with enhanced high-frequency oscillatory intensity in the rostral brain rather than enhanced low-frequency oscillatory activity in the caudal brain, which is often found in patients with dementia [30, 74,75,76,77]. The difference in frequencies (i.e., delta and high gamma) implies that different types of neural mechanisms underlie the neural activity in the regions [44,45,46,47,48,49,50,51], further suggesting that cognitive dysfunction-related oscillatory changes in the right insular cortex and right middle temporal gyrus are not the same as oscillatory changes in patients with 'genuine dementia'. The changes we observed should be considered pain-related transient changes because (1) brain regions affected by pain and cognitive dysfunction are close to each other and may even overlap (Fig. 2), (2) the oscillatory changes were only observed before SNRB (i.e., when the patients experienced severe pain), and (3) high- and low-frequency oscillatory activity often interact with each other (cross-frequency coupling) [78]. High-frequency oscillatory activity represents regional activity, while lower-frequency oscillatory intensities are mainly produced by the distributed network [44,45,46,47,48,49,50,51]. High-frequency oscillatory activity in the right hemisphere is enhanced by the administration of non-pharmacological treatment to patients with dementia, which improves the patients' scores on the MMSE-J [31]. We speculate that high-frequency oscillatory activity compensated for the pain-induced cognitive dysfunction in these patients. The chief complaint of the participants was sciatic pain, and they did not notice their cognitive dysfunction in their daily lives. In other words, living their ordinary lives stimulated their brain and thus resembled a type of non-pharmacological treatment. Their engagement in daily activities enhanced high-frequency oscillatory intensity and effected a compensatory mechanism. The enhanced high-frequency oscillatory activity—i.e., the compensation—disappeared after SNRB, likely because the pain no longer disturbed the cognitive system. Although the high-frequency oscillatory activity could represent the preclinical state of dementia (i.e., transient cognitive dysfunction), it could lead to dementia due to cross-frequency coupling [78]. Therefore, in order to prevent clinical cognitive impairment, it is important to address any experienced pain.

At the group level, regional oscillatory intensities did not show significant changes across SNRB at any frequency in any participant (red arrow in Fig. 1). This finding reflects the fact that pain is processed by a widely distributed cortical/subcortical network (e.g. 'pain matrix') rather than a single brain region [1, 10]. Individual differences in changes in regional oscillatory intensities would cancel out between participants.

The present study was subject to several limitations. First, pain is typically classified into binary categories of acute and chronic pain. We did not distinguish between them because it is difficult to determine whether sciatic pain is acute or chronic. Second, we did not divide the participants into subgroups based on the side of the body or type of body parts in which the pain was experienced. Although we used the participants' self-report to determine in which body parts they felt pain, most described the location ambiguously. The inaccuracy of the reported locations of pain could be due to 'non-specific, functional, and somatoform bodily complaints', which implies that pain is distributed across several locations, being a principle symptom of the patient’s conditions [79, 80]. Third, enhanced low-frequency oscillatory activity due to cognitive decline was not observed in the present study. We speculate that no participants suffered from severe cognitive decline, and the change was therefore not statistically significant. Fourth, cognitive status was assessed using MMSE-J only after SNRB. It is impractical to assess the same participant twice using the same neuropsychological assessment, since the participants always perform better on the second assessment (i.e., practice effect). In addition, from an ethical perspective, it is not appropriate to request that a patient in pain perform a task. Fifth, subjective and cognitive assessments did not show a direct relationship between the subjective level of pain and cognitive status either before or after SNRB. We ascribe this finding to selection bias. In the present study, we only enrolled patients who were scheduled to receive SNRB due to severe pain. Hence, patients with mild pain were not recruited (average and SD of pain-VAS readings were 7.32 ± 2.02 before the SNRB). Therefore, whether patients with mild or no cognitive impairment feel relatively less pain warrants further study in future research.


The activity of the pain system (i.e. 'pain matrix') was modulated by enhanced low-frequency oscillatory activity, and cognitive dysfunction under severe pain was related to enhanced high-frequency oscillatory activity. The regions in which these two oscillatory activities were observed overlapped in the right hemisphere; however, all oscillatory activity disappeared after the treatment of the pain with SNRB. It is plausible that these neural activities represent the transient bridge between pain and cognitive dysfunction. Therefore, our findings underscore the importance of pain before the transient bridge persists and aggravates cognitive dysfunction.