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Morphological and Molecular Characterization of Ectomycorrhizal Amanita Species Associated with Pinus wallichiana A. B. Jacks.

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Worldwide genus Amanita is represented by more than 500 ectomycorrhizal (ECM) species. These species form mutualistic associations with a number of host trees among which the conifers predominate. Set out to explore the ECM associations of Amanita in Kashmir Himalayas, Amanita calyptroderma, Amanita pantherina and Amanita vaginata show symbiotic associations with the roots of Pinus wallichiana. Besides morphological characterization of mycorrhizal roots and sporocarps, molecular characterization of rDNA ITS region was performed for identification of species. ITS-rDNA was used for molecular analysis. The target region of rDNA was amplified using universal fungal primers (ITS1 and ITS4). The sequencing of amplified products and their subsequent blast analysis confirmed the identification of species by comparing the sequences of these species with sequences present in GenBank. Phylogenetic analysis also confirmed the identification of species.

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References

  1. Smith SE, Read DJ (2008) Mycorrhizal Symbiosis, 3rd edn. Academic Press, London

    Google Scholar 

  2. Itoo ZA, Reshi ZA (2013) The multifunctional role of ectomycorrhizal associations in forest ecosystem processes. Bot Rev. doi:10.1007/s12229-013-9126-7

    Google Scholar 

  3. Rinaldi AC, Comandini O, Kuper TW (2008) Ectomycorrhizal fungal diversity separating wheat from the chaff. Fungal Divers 33:1–45

    Google Scholar 

  4. Zhang L, Yang J, Yang Z (2004) Molecular phylogeny of eastern Asian species of Amanita (Agaricales, Basidiomycota): taxonomic and biogeographic implications. Fungal Divers 17:219–238

    Google Scholar 

  5. Pande V, Palni UT, Singh SP (2004) Species diversity of ectomycorrhizal fungi associated with temperate forest of Western Himalaya: a preliminary assessment. Curr Sci 86:1619–1623

    Google Scholar 

  6. Watling R, Abraham SP (1992) Ectomycorrhizal fungi of Kashmir forests. Mycorrhiza 2:81–87

    Article  Google Scholar 

  7. Ingleby K, Mason PA, Last FT, Fleming LV (1990) Identification of ectomycorrhizas. Institute for Terrestrial Ecology, Natural Environmental Research Council, London

    Google Scholar 

  8. Agerer R (1994) Index of unidentified ectomycorrhizae III. Names and identifications published in 1992. Mycorrhiza 4:183–184

    Article  Google Scholar 

  9. Sette LD, Passarin MRZ, Delarmelina C, Salati F, Duarte MCT (2006) Molecular characterization and antimicrobial activity of endophytic fungi from coffee plants. World J Microbiol Biotechnol 22:1185–1195

    Article  CAS  Google Scholar 

  10. Peintner U, Moser MM, Thomas KA, Manimohan P (2003) First records of ectomycorrhizal Cortinarius species (Agaricales, Basidiomycetes) from tropical India and their phylogenetic position based on rDNA ITS sequences. Mycol Res 107:485–494

    Article  CAS  PubMed  Google Scholar 

  11. Manassila M, Nguan TS, Boonkerd N, Rodtongb S, Teaumroonga N (2005) Phylogenetic diversity of wild edible Russula from Northeastern Thailand on the basis of internal transcribed spacer sequence. Sci Asia 31:323–328

    Article  CAS  Google Scholar 

  12. Itoo ZA, Reshi ZA, Andrabi KI (2013) Characterization and identification of Russula firmula and Russula postiana from Himalayan moist temperate forests of Kashmir. Afr J Biotechnol 12:3643–3647

    CAS  Google Scholar 

  13. Atri NS, Kaur A, Kaur H (2003) Wild mushrooms—collection and identification. Chambaghat, Solan

    Google Scholar 

  14. Higgins DG, Bleasby AJ, Fuchs R (1992) CLUSTAL V: improved software for multiple sequence alignment. Comput Appl Biosci 8:189–191

    CAS  PubMed  Google Scholar 

  15. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Felsenstein J (1995) PHYLIP—phylogenetic inference package, version 3.5c. Computer programs distributed by the author. Department of Genetics, University of Washington, Seattle

    Google Scholar 

  17. Felsenstein J (1985) Confidence intervals on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  18. Avin FA, Bhassu S, Shin TY, Sabaratnam V (2012) Molecular classification and phylogenetic relationships of selected edible Basidiomycetes species. Mol Biol Rep 39:7355–7364

    Article  CAS  PubMed  Google Scholar 

  19. Siddiquee S, Tan SG, Yusuf UK, Fatihah NH, Hasan MM (2012) Characterization of Malaysian Trichoderma isolates using random amplified microsatellites (RAMS). Mol Biol Rep 39:715–722

    Article  CAS  PubMed  Google Scholar 

  20. Gonzalez P, Labare`re J (2000) Phylogenetic relationships of Pleurotus species according to the sequence and secondary structure of the mitochondrial small-subunit rRNA V4, V6 and V9 domains. Microbiology 146:209–221

    Article  CAS  PubMed  Google Scholar 

  21. Ito Y, Fushimi T, Yanagi SO (1998) Discrimination of species and strains of basidiomycete genus Coprinus by random amplified polymorphic DNA (RAPD) analysis. Mycoscience 39:361–365

    Article  CAS  Google Scholar 

  22. Shnyreva A, Shtaer O (2006) Differentiation of closely related oyster fungi Pleurotus pulmonarius and P. ostreatus by mating and molecular markers. Russ J Genet 42:539–545

    Article  CAS  Google Scholar 

  23. Choi DB, Ding JL, Cha WS (2007) Homology search of genus Pleurotus using an internal transcribed spacer region. Korean J Chem Eng 24:408–412

    Article  CAS  Google Scholar 

  24. Nilsson RH, Kristiansson E, Ryberg M, Hallenberg N, Larsson KH (2008) Intraspecific ITS variability in the kingdom Fungi as expressed in the international sequence databases and its implications for molecular species identification. Evol Bioinform 4:193–201

    Google Scholar 

  25. White T, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  26. Kulmann C, Kim SJ, Lee SS, Harms C (2003) A reliable, “Direct from Field” PCR method for identification of mycorrhizal fungi from associated roots. Mycobiology 31:196–199

    Article  CAS  Google Scholar 

  27. Reddy MS, Singla S, Natarajan K, Senthilarasu G (2005) Pisolithus indicus, a new species of ectomycorrhizal fungus associated with Dipetrocarps in India. Mycologia 97:838–843

    Article  CAS  PubMed  Google Scholar 

  28. Itoo ZA, Reshi ZA (2013) Effect of different nitrogen and carbon sources and concentrations on the mycelial growth of ectomycorrhizal fungi under in vitro conditions. Scand J For Res. 10.1080/02827581.2014.964756

  29. Itoo ZA, Basharat Q, Majeed ST, Andrabi KI, Reshi ZA (2014) Ectomycorrhizal fungal species of Kashmir Himalaya: identification and characterization by ITS analysis. Braz J Bot. 10.1007/s40415-014-0081-2

  30. Hortal S, Pera J, Galipienso L, Parlad´e J (2006) Molecular identification of the edible ectomycorrhizal fungus Lactarius deliciosus in the symbiotic and extraradical mycelium stages. J Biotechnol 126:123–134

    Article  CAS  PubMed  Google Scholar 

  31. Iotti M, Zambonelli A (2006) A quick and precise technique for identifying ectomycorrhizas by PCR. Mycol Res 110:60–65

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors greatly appreciate Senior and Junior research fellowships in favour of Sheikh Tahir Majeed (09/251/0055/2013/EMRI) and Qussin Basharat (09/251/0045/2011/EMRI) from Council of Scientific and Industrial Research (CSIR) respectively. Special thanks to Sheetal Ambardar (School of Biotechnology University of Jammu) for her help in developing the phylogenetic analysis and providing valuable comments. The authors entitled to the authorship do not have any conflict of interest.

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Authors and Affiliations

  1. Biological Invasions Research Lab, Department of Botany, University of Kashmir, Srinagar, 190006, India

    Zahoor Ahmad Itoo & Zafar A. Reshi

  2. Department of Biotechnology, University of Kashmir, Srinagar, 190006, India

    Zahoor Ahmad Itoo, Sheikh Tahir Majeed, Qussin Basharat & Khurshid Iqbal Andrabi

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  1. Zahoor Ahmad Itoo
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  2. Zafar A. Reshi
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  3. Sheikh Tahir Majeed
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  4. Qussin Basharat
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  5. Khurshid Iqbal Andrabi
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Correspondence to Zahoor Ahmad Itoo.

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Itoo, Z.A., Reshi, Z.A., Majeed, S.T. et al. Morphological and Molecular Characterization of Ectomycorrhizal Amanita Species Associated with Pinus wallichiana A. B. Jacks.. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 985–994 (2016). https://doi.org/10.1007/s40011-015-0527-8

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  • DOI: https://doi.org/10.1007/s40011-015-0527-8

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