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Effects of Thidiazuron (TDZ) on Direct Shoot Organogenesis of Gymnocladus assamicus: A Threatened and Critically Endangered Species from Northeast India

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Abstract

An efficient morphogenic potential was developed for direct shoot organogenesis of Gymnocladus assamicus, as an IUCN Red List of threatened and critically endangered species from Northeast India. This species is used as leech repellent of domestic animal, seedpod as detergent and roasted seed as a substitute for coffee and groundnut. The wild population is rapidly shrinking due to various anthropogenic pressure and poor regeneration. Therefore, the present study has been taken up for morphogenic potential through direct shoot organogenesis which is not reported. Nevertheless, cotyledonary nodal explants showed 100% responses in Murashige and Skoog (MS) medium fortified with 0.75 mg L−1 thidiazuron (TDZ) alone or in combination with 1 mg L−1 IBA, in comparison with other combinations tested. Cotyledonary node was found to be the best source of explant which produced 10.80 ± 0.39 shoots per explant. Further, shoots were transferred to proliferation and elongation medium fortified with 0.25 mg L−1 TDZ in MS medium which produced 12.06 ± 0.31 shoots per explant. MS medium fortified with 1.5 mg L−1 IAA showed highest root induction frequency (76%) with mean root number 2.03 ± 0.19 and root length 3.26 ± 0.27 cm. The micropropagated plantlets were transferred to soil after acclimatization with a 68% success rate.

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Acknowledgements

All authors are thankful to the Head of Office, BSI, Shillong, for providing necessary facilities, and the Director, BSI, Kolkata, for giving financial support and constant encouragement.

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Authors and Affiliations

  1. Andaman and Nicobar Centre for Ocean Science & Technology (ANCOST), National Institute of Ocean Technology (NIOT), Industrial Estate Road, Dollygunj, Port Blair, Andaman and Nicobar Islands, 744103, India

    Sanjoy Gupta

  2. Botanical Survey of India, Eastern Regional Centre, Shillong, 793003, India

    Ashiho Asossi Mao

  3. Department of Biotechnology, Gauhati University, Guwahati, Assam, 781014, India

    Soneswar Sarma

Authors
  1. Sanjoy Gupta
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  2. Ashiho Asossi Mao
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  3. Soneswar Sarma
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SG implemented all the experiments, analyzed the data and written the manuscript. AAM and SS planned the work, edited the manuscript and monitored the entire research work.

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Correspondence to Sanjoy Gupta.

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Gupta, S., Mao, A.A. & Sarma, S. Effects of Thidiazuron (TDZ) on Direct Shoot Organogenesis of Gymnocladus assamicus: A Threatened and Critically Endangered Species from Northeast India. Natl. Acad. Sci. Lett. 43, 85–91 (2020). https://doi.org/10.1007/s40009-019-00801-5

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  • DOI: https://doi.org/10.1007/s40009-019-00801-5

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