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Plantlet Regeneration via Multiple Shoot Induction in Indian Cultivars of Lucerne (Medicago sativa L)

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Abstract

An efficient protocol has been developed for in vitro plant regeneration via multiple shoot induction in lucerne (Medicago sativa L). Shoot tips from in vitro grown 5–6 days old seedlings of 3 cultivars, LLC-3, Chetak and RL-88 were used as explants for multiple shoot induction on MS medium supplemented with cytokinins. Maximum of 14 shoots per apical meristem were observed in case of cv Chetak on MS medium supplemented with BAP (12.6 μM) and KN (9.3 μM). Shoot elongation on MS medium supplemented with GA (5.8 μM), while root induction was achieved on MS medium supplemented with IAA (11.4 μM) and activated charcoal (2.0 g l−1). Tissue raised plants showed 75% survival after transfer to soil under field conditions.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

indole-3-acetic acid

KN:

kinetin

MS:

Murashige and Skoog

GA:

gibberellic acid

NAA:

α-naphthaleneacetic acid

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Authors and Affiliations

  1. Division of Crop Improvement, Indian Grassland and Fodder Research Institute, Jhansi, 284 003, India

    Suresh Kumar

Authors
  1. Suresh Kumar
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  2. Amaresh Chandra
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  3. Madan G. Gupta
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Correspondence to Suresh Kumar.

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Kumar, S., Chandra, A. & Gupta, M.G. Plantlet Regeneration via Multiple Shoot Induction in Indian Cultivars of Lucerne (Medicago sativa L). J. Plant Biochem. Biotechnol. 17, 181–184 (2008). https://doi.org/10.1007/BF03263282

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  • DOI: https://doi.org/10.1007/BF03263282

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