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Anti-Osteoarthritic Effects of Cartilage-Derived Extracellular Matrix in a Rat Osteoarthritis Model

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

BACKGROUND:

The extracellular matrix (ECM) has many functions, such as segregating tissues, providing support, and regulating intercellular communication. Cartilage-derived ECM (CECM) can be prepared via consecutive processes of chemical decellularization and enzyme treatment. The purpose of this study was to improve and treat osteoarthritis (OA) using porcine knee articular CECM.

METHODS:

We assessed the rheological characteristics and pH of CECM solutions. Furthermore, we determined the effects of CECM on cell proliferation and cytotoxicity in the chondrocytes of New Zealand rabbits. The inhibitory effect of CECM on tumor necrosis factor (TNF)-α-induced cellular apoptosis was assessed using New Zealand rabbit chondrocytes and human synoviocytes. Finally, we examined the in vivo effects of CECM on inflammation control and cartilage degradation in an experimental OA-induced rat model. The rat model of OA was established by injecting monosodium iodoacetate into the intra-articular knee joint. The rats were then injected with CECM solution. Inflammation control and cartilage degradation were assessed by measuring the serum levels of proinflammatory cytokines and C-telopeptide of type II collagen and performing a histomorphological analysis.

RESULTS:

CECM was found to be biocompatible and non-immunogenic, and could improve cell proliferation without inducing a toxic reaction. CECM significantly reduced cellular apoptosis due to TNF-α, significantly improved the survival of cells in inflammatory environments, and exerted anti-inflammatory effects.

CONCLUSION:

Our findings suggest that CECM is an appropriate injectable material that mediates OA-induced inflammation.

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Acknowledgements

Everyone who contributed significantly to the work has been listed. This research was supported by the National Research Foundation Grant (NRF- 2019M3E5D1A02070861.)

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Authors and Affiliations

  1. Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan, Republic of Korea

    Sang-Hun Lee, Sung-Han Jo, Seon-Hwa Kim & Sang-Hyug Park

  2. The Center for Marine Integrated Biomedical Technology (BK21 PLUS), Pukyong National University, Busan, Republic of Korea

    Sang-Hun Lee, Sung-Han Jo, Seon-Hwa Kim & Sang-Hyug Park

  3. Department of Orthopedics Surgery, Kosin University Gospel Hospital, 45 Yongso-Ro, Nam-Gu, Busan, Republic of Korea

    Chang-Su Kim

  4. Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence, Pukyong National University, Busan, Republic of Korea

    Sang-Hyug Park

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Correspondence to Sang-Hyug Park.

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All animal studies were approved by the Institutional Review Board at the Pukyong National University (PKNUIACUC-2020–03).

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Lee, SH., Jo, SH., Kim, SH. et al. Anti-Osteoarthritic Effects of Cartilage-Derived Extracellular Matrix in a Rat Osteoarthritis Model. Tissue Eng Regen Med 20, 83–92 (2023). https://doi.org/10.1007/s13770-022-00508-7

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