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Combined Application of Dentin Noncollagenous Proteins and Odontogenic Biphasic Calcium Phosphate in Rabbit Maxillary Sinus Lifting

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Teeth can be used as a raw material for preparing bone substitutes due to their similar chemical composition to bone. The objective of our study was to evaluate the effect of odontogenic biphasic calcium phosphate (BCP) incorporating dentin noncollagenous proteins (DNCPs) on osteogenesis and stability in maxillary sinus augmentation.

Methods:

The composition, structure and morphology of the odontogenic BCP were tested by X-ray powder diffraction (XRD), Brunauer–Emmett–Teller, and scanning electron microscopy methods. The biocompatibility and osteoinduction of DNCPs and materials were examined in vitro and their bone regeneration capacity was verified in vivo.

Results:

The results showed that the cells adhered and proliferated well on the DNCP-loaded BCP scaffold. The odontogenic BCP and DNCPs promoted osteogenic differentiation of cells, The new bone formation in the BCP groups and DNCP subgroups was significantly higher than the new bone formation in the control, and the new bone quality was better. The bone regeneration effect of odontogenic BCP was similar to the effect of deproteinized bovine bone mineral, but β-TCP did not maintain the height and volume of bone reconstruction.

Conclusion:

In conclusion, the combined application of DNCPs and odontogenic BCP is an effective strategy for tissue engineering osteogenesis in the maxillary sinus region. The biomimetic strategy could provide a new approach for patients requiring maxillary sinus lifting.

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Acknowledgements

The authors are thankful to Center for Scientific Research and Key Lab. of Oral Diseases Research of Anhui Medical University for supporting the research. This work was supported by the Anhui Provincial Key Research and Development Plan (201904a07020062). Anhui Provincial Key Research and Development Plan, 201904a07020062, Yuanyin Wang. 2021Disciplinary Construction Project in School of Dentistry, Anhui Medical University 2021kqxkFY10.

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Author notes

  1. Gang Yang and Xin Liu contributed equally to this work and should be considered co-first authors.

Authors and Affiliations

  1. Key Laboratory of Oral Diseases Research of Anhui Province, Oral and Maxillofacial Surgery, Stomatologic Hospital & College, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China

    Gang Yang, Tianyu Huang, Ruyuan Ding & Yuanyin Wang

  2. Key Laboratory of Oral Diseases Research of Anhui Province, Department of Oral Implant, Oral and Maxillofacial Center, Stomatologic Hospital & College, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China

    Xin Liu

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  1. Gang Yang
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Correspondence to Xin Liu or Yuanyin Wang.

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The animal studies were performed after receiving approval of the Animal Research Committee of Anhui Medical University (LLSC 20210681).

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Yang, G., Liu, X., Huang, T. et al. Combined Application of Dentin Noncollagenous Proteins and Odontogenic Biphasic Calcium Phosphate in Rabbit Maxillary Sinus Lifting. Tissue Eng Regen Med 20, 93–109 (2023). https://doi.org/10.1007/s13770-022-00502-z

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